Preparation of Competent Cells Using Calcium Chloride - Amrita University

[@spkm4tube]

Hi, you may add little bit of DMSO of glycerol before freezing, for details please see Sambrook protocol 23 step 12

[@sharon246787]

very good...but y is calcium chloride used y not any other divalent cation?

[@1pinkiya]

excellent description

[@TBrewer64]

What are the concentrations of the solutions?

[@sw-hd3ez]

impressive presentation! wow...

[@english806]

thanks