Self made chemocompetent cells---5min on ice with DNA-Heat shock-5min ice-45min shaking at 37deg--plating as desired! (works best for me since 3 years!)
Hi Slimane DzIsnt, Thank you for your question. 15ul should be sufficient to get few colonies, but usually 100ul is the normal. For additional technical support, please contact us at thermofisher.com/askaquestion. Thank you!
Self made chemocompetent cells---5min on ice with DNA-Heat shock-5min ice-45min shaking at 37deg--plating as desired!
(works best for me since 3 years!)
i want to work for invitrogen
Nicely explained 👍
Thank you for watching! Glad you liked the video.
Which occupation is this?
Biotechnology 😊
Isnt 15 microliter too little to plate on selective LB?
Hi Slimane DzIsnt, Thank you for your question.
15ul should be sufficient to get few colonies, but usually 100ul is the normal.
For additional technical support, please contact us at thermofisher.com/askaquestion.
Thank you!
@@thermofisher Thank you for the answer
can you get a charge from a dead computer batory
Nice
Nice but . Wat u dng.
Long story short bioshock