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Thanks for the interesting video. Are you from the San Diego area? Your Name sounds familiar .

Will it stay purple after long years of sun exposure?

I always knew this lichen was special. Although, ammonia is not something I like to mess with. Pokeberries make purple too.

Is the isopropanol ice cold or room temparatyre

Hello thanks for your video, i am now fermenting my own! will this colour fade in sunlight?

Absolutely mesmerised. Thank you for this video, and your dedication to keeping an ancient craft alive.

Does it need to be heated ?

I added lime of milk. Does this affect the target product?

Thankyou sir ❤

Is there a need to (pre)mordant, or is there enough tannins in the lichen? If there is a need for premordant, what would give the best effect for purple?

Gorgeous! My MIL was cleaning graves on Memorial Day this year and i asked her to save any lichen she found. I was expecting some of the xanthoria lichens because she described it as bright yellow-green, but instead she brought back nearly a quart of this! Looking forward to trying it out.

you should have a rack under the glass jar - like they do with canning.

It is a beautiful color, but you'd have to collect & use a LOT of lichen for just 1 garment.

GREAT ! You show us pictures of the lichen and how to test it.

GREAT video!

How do u prevent scraping agar while removing mycelium from plate The fungi im working on has very flat colony

great video

is it lightfast?

Last summer, I made this according to your directions and it worked great! Trying it again and after three weeks I have what looks like grape juice colored solution. My question is: How do I know when it's ready?

Thank you Julian

This is amazing. I never heard of this process. Thanks for sharing.

💜💜💜💜Yaaay I love the color purple and almost exclusively wear only that. Thank you:) am exciting to go through the process soon

No enzymatic digestion, no incubatiom on water bath. Just addind ctab and cloroform and thats it????. I doubt it

Which kind of malt extract use Diastatic or non diastatic

I'm pouring in a still air box. Can I leave the lids off the same way until the agar cools or will there be a risk of contamination? I don't have a flow hood.

I have looked everywhere for a "soup to nuts" method of using lichen as a die and you have done it! I also live on the east coast and I thought that the lichen that I had collected was the common Green Shield (Flavoparmelia caperata) but your instructions on using a drop of bleach verified what I had initially thought was Punctelia rudecta. Yay! Purples!!! I love your step by step directions from harvesting, removal from bark, and the beginning to the end of the dying process. Thank you so much for an incredibly informative video! A++ !

What an astonishing color! Is it colorfast?

Awesome, thank you for informationz

I have a question. I started fermenting lichen and now realize I have so much more than I need. Will it keep longer than 6 months if I leave it in the jar? If I want to keep it longer should I strain out the lichen and boil the dye before saving it for later. Thanks for the info in the video so far. My wood pile was covered in the stuff and I got a bit overexcited, which is why I now have way more dye than I need at a time.

Hi Julian. I have had failed DNA extractions. Using the lysis buffer composed 100 mM Tris, 250 mM KCl, and 10 mM disodium EDTA (pH 9.5). The samples in tube PCR to 95°C for 10 min and then mixed with 40 ul BSA 3%. I tested in a electrophoresis the DNA and apparently there is no DNA and in PCR I can´t see amplicons. The fungal tested in my case were Beauveria, Metarhizium, Diplodia and Clonostachys. Considering your experience what is the probably problem ??

Thanks for the protocol. Could you mention the code of the BSA solution you used or did you prepare it from powder?

Very beautiful shade. 💜

Beautiful! Is it the Stone-Lichen I used to make a beautiful rich reddish-Brown colour, with chemicals Alun and vinsten (Sw)? For this fine purple: No further chemicals needed for permanence?

Very useful thank you. Confirmed that all of my seeds were indeed unviable!!

Would it be possible to make ink with lichen like this? And if so must it smell of ammonia? How would you turn it into an ink?

I collected lots of lichen on fallen trees on my propety, and it had been soaking in water and ammonia for 2 months now, and no sign of purple at the moment. The liquid is dark brown with some dark red hues, but it yields some uninteresting brownish dirty tone. Do you think it will really turn purple in a couple more months or it should have already started to look like purple is on the way?

Can you please share any reference of this protocol? I want to cite this method. Thanks.

Thank you for this tutorial! It was very fascinating!

I wonder if it would work as a hair tones

Thank you for this video! I‘ve never seen such a interesting color dyeing idea from lichen. Also it’s interesting that you use to gather lichen chopsticks and a tofu package and cook it with a rice cooker😊

How interesting! Would be great to know how this dye was “discovered” (I.e. using the ammonia to extract the pigment).

If you use any acid like citric acid it will be pink or red, is that what you said? I couldn’t hear exactly. Thanks.

i have a request please, can you show us how to extract DNA from plants infected with obligatory fungi like Rust and Smut ??

Can i use this receipe to make liquid culture medium by doing the same thing minus the agar?

You are Godsend. Thank you so much. By the way, do you have a citation for your source of protocol? thank you

This is my first time to understand I failed and failed and failed all times to grow mushroom thanks a lot

You are successful teacher

After all this I think I would rather buy some purple yarn at the store! 😅

thanks for the video. why did you make a single cutlet at the end when the whole plate is still used?

How to prevent the colour by fading?