Antinuclear antibody screening - Making an informed decision
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- Опубліковано 24 лип 2024
- Antinuclear antibody screening - Making an informed decision
Gyorgy Abel, MD, PhD, DABCC, Director of Clinical Chemistry, Molecular Diagnostics, and Immunology, Lahey Hospital & Medical Center
In addition to his responsibilities in the clinical laboratory, Dr. Abel also serves as Instructor in Pathology, part-time, at Harvard Medical School in Boston. He has made significant contributions to HCV research with a seminal study demonstrating the use of LDL receptor by the virus as a port of entry into hepatocytes. He is the author or co-author of over 100 articles, book chapters, and scientific conference presentations, and a regular reviewer for scientific journals.
Melissa R. Snyder, PhD, DABCC, Co-Director, Antibody Immunology Laboratory, Mayo Clinic
Melissa R. Snyder, PhD completed her doctoral degree in Biochemistry through the Mayo Graduate School in 1997. She followed that with a post-doctoral fellowship in clinical/basic immunology, during which time she studied T-cell function in autoimmune disease, specifically rheumatoid arthritis. She then completed a clinical chemistry fellowship at the Mayo Clinic, where she focused on laboratory diagnostics and autoimmune disease. After completing her clinical fellowship, she was board-certified in Clinical Chemistry by the American Board of Clinical Chemistry. Dr. Snyder is currently Co-Director of the Antibody Immunology Laboratory at the Mayo Clinic, with an academic appointment as Associate Professor of Laboratory Medicine and Pathology.
Antinuclear antibody screening - Making an informed decision
Testing for antinuclear antibodies (ANAs) has been a routine part of the evaluation of patients with a suspected connective tissue disease (CTD) for the last 50 years. Dr. Melissa Snyder and Dr. Gyorgy Abel will present the methods for ANA testing available to the clinical laboratory today as well as current ANA testing guidelines and examples of algorithms in use. Dr. Snyder will present findings from her study comparing various ANA methods and Dr. Abel will share his laboratory’s experience in implementing one of the newer methods for ANA testing. The speakers will provide valuable information to help you make an informed decision for your laboratory when choosing methods for ANA testing.
Learning Objectives:
• List the methods available to the clinical laboratory for general ANA testing.
• Describe ANA testing guidelines and algorithms and the current trends in testing.
• Describe how the various ANA methods compare in terms of analytical characteristics and clinical utility.
Sponsored by: Bio-Rad Laboratories
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Im studying to become a Medical Laboratory Scientist and am very grateful for this video!
MLT
@@lydiacordero5245 I'm a fetus and I'm studying to be a lab assistant. Thanks!
Thank you for this informative video.
Dismissive/Arrogant, empathy-deficient doctors and other medical staff need to see this video.
After dealing with several (in my opinion - inept) gp doctors for 2 months I finally told one of the dismissive doctors. I know that I have Lupus. The obvious butterfly rash should have warranted tests for this 2 months ago alone. Now can you give me a blood test for ANA, an ENA panel, and anti-dsdna already because I’m sick of this. I’m sorry, I know it’s not my place to tell doctors what I have but I’ve known this for at least a month and continuously explained my symptoms that aligned with this many times hoping you guys would look into it without prompting. But nobody has.
I was an arrogant dick and lucky not to just be thrown out by a relatively awkward mild mannered young Asian doctor, but to my delight he agreed.
Today came back ANA positive and anti-dsdna positive. Sorry for the story wanted to vent.
If you know something is wrong with you, don’t give up when doctors tell you basically not to worry about all your symptoms and you’ll get better
Thanks for these presentations, perfect 👍
Very informative...
Thanks
Mixed patterns can be also observed in.. what we can do for diagnosis in this case?
It's so usefull thank you very much indeed👍♥️
FInally found a clear explanation for ANA staining IFA patterns. Thank you!
I had 2 different staining patterns first one at 1to 1:160 titer/pattern Homogeneous and a second pattern also at 1:160 was speckled testing was ANA by IFA, HEp, 2000 substrate, with polyarthritis equal on both sides of body, no joint erosions typically seen with rheumatoid disease and with possible kidney disease stage 2/3 and with albumin now being detected is possible inflammatory disease of the kidneys does this look more like late onset lupus than rheumatoid disease, malar rash isn't commonly seen as much in late onset lupus than in early onset lupus any replies would be appreciated thanks.
great
Speckled pattern. Is that possible lupus can be misdiagnosed ?
I have a rare bridging...mitotic intercellular bridge
My ANA is 1:160
Can anyone explain why my bridging is considered abnormal?
Is it indicative of disease?
My Ana is speckled 1:160 end
My hair is dry
Rosacea like skin
Cold hands and feet
Fatigue sleep issues
Dry everywhere inside and out
Mouth only dry when waking
Told nothing wrong and I’m really getting depressed
Sounds like sjorgens
Read my comment from today.
It could definitely be lupus. I’ve definitely had it for years. Joint pain only just recently started. Frequent infections. Another basic blood test they did showed high red blood cells (it can also show low for lupus but that’s irrelevant).
I finally demanded an ANA test and a ENA panel and a anti-Dsdna test (as the ENA test doesn’t do this alone). Doctor came to his senses and agreed and today I was finally diagnosed with lupus, positive Ana and anti-dsdna.
Now checking kidney function with a urine test as one of my symptoms is on and off frequent urination at night (like 5-10 times waking from sleep) and occasional frothy urine
Sorry, overlooked the fact you’re already Ana positive. Have an anti-dsdna test if you haven’t already. It’s likely they did a ENA panel, but most of these tests are unlikely to show up if you have lupus, like one or two that only 30% of people with lupus have.
You may still be negative for anti-dsdna and have lupus as it’s only in 30-75% of cases.... but still.
The fact you’re ANA positive and have these symptoms is strongly indicative alone you likely have an auto-immune disease.
My obvious butterfly rash was wrongly attributed to roseacea which I’ve never had, and only came on with a huge flare up. Doctors now agree it’s a butterfly rash and not roseacea... even though it’s not the most common looking butterfly rash rash - still closer to that than roseacea
Wish you health & peace my friend!
Male Ana 1:640 homogeneous, slightly elevated antilipid all other blood work perfect. Sm and dsdna neg. But I have malar rash, rashes on body with sun exposure, night sweats and fever for years, tested positive as a child for too much protein in urine, joint pains, neurological symptoms, chest pain, kidney pain, mouth ulcers, etc and still no diagnosis and dr says it’s nothing you are perfectly healthy. Wth!!
Could you rule out the vaccines as a major factor contributing to Autoimmunity ??
Can vaccines over activate immune system to the point of confused reaction against body's organs ??
Thats a subject no one wants to touch. It is designed to directly illicit an immune response, so that possibility should be considered.
dsDNA what illnesses?
SLE
good
Hai, i have ANA 2+mildly positive with titer 1:320 , ANA REFELX ENA positive, were as ds dna , neculesome, histone ,SM , nRNP/sm , RO-52,SS-A,SS-B,SP-100,PML,SCL 70, PMSCL -75 100, RP 11, RP 155,CENP A B ,KU, Mi -2 alpha beta ,Gp 210, PCNA , DFS70 -
all test are negative ,
apla -neg,
LA - NEG ,
ACLA iga,m,g - negative ,ESR- normal
wbc normal
platelets normal
Beta2 igm, iga , igg - negative .
i have this. can i take the covid 19 vaccination? will it mess up my immune system worse then it is now? which vaccine should i take - Pifzer or Sinovac?
None. People with autoimmune diseases cannot take them.
@@RM-qq5rj already taken Sinovac
@@chuaailing99 I hope and pray for the best outcome for you!
None!
Did the narrator actually say "nucular?" I know she unbelievably said Mye - oh Clinic! 😳
She said "nucular"........