Amazing videos on prokaryotic transcription, I've enjoyed them so much :) I appreciate the approach with making such lessons based on scientific articles, not outdated textbooks. Could you please leave references to such articles if possible?
Hello. I am glad the content is helpful. I can try to make a compilation of papers I have read overtime (which I think will take more time than I have at the moment) but I worry that the material simply may be overwhelming. This video alone was summarized through at least 10 or so papers - at some point I will get to making a list of my sources :) Thanks for the suggestion - I will try to implement this in my upcoming videos if possible :)
Very nice and neat. I suggest you add complementary video speaking about the two possible tracking mechanism suggested for Rho-helicase: Tether tracking and Rut-free tracking.
For the rRNA and tRNA do they use the intrinsic termination mechanism to undergo termination? As rRNA has a NUT site that blocks the Rho protein, let's say in intrinsic mechanism, the NUT site will be blocked outside of the transcription bubble by the Trigger loop, and the RNA: DNA hybrid is broken by the lid and rudder at the exit of RNA and DNA. Hope to get some feedback 🫶🏻
Did you explained the RNA polymerase proofreading mechanisms somewhere... If so please tell me. BTW the videos are amazing, I learned the whole mRNA trasncription from here.. thanks to you I was able to prepare for my MGenetics Quiz.
The hairpin forms because there is internal complementarity in the RNA/DNA - i.e. the definition of a hairpin. You don't need external factors to form a hairpin - maybe to stabilize it, but not to form it.
Why doesn't nus factor prevent secondry structure formation at termination site during rho independent process since nus do it during elongation process...plz explain
NusG (typically) is the anti-terminator/elongation factor. But NusA is a termination factor. So not all Nus factors prevent secondary structures. Also, recent studies show that NusG can also bind to certain sites and stabilize secondary structures and thus, promoting secondary structure formation.
Hello! The subtitle generation (in English) is done by UA-cam itself (I think long videos are difficult to auto-caption). Unfortunately, I do not have a written script for these videos. I apologize for the inconvenience.
I do not know, why the channel does not have at least several hundred thousands subscribers
I am studying for my final exams and this is finally one of the greatest video I have ever seen as a educational material. Thank you!
Superb explanation. Best video in in depth explanation in a simple understandable language
Awesome sir
The pronounciation is also so clear
Termination is now clear
Thank you sir
I am glad the video was useful 👍
Great video! looking forward to your translation videos!
Amazing videos on prokaryotic transcription, I've enjoyed them so much :)
I appreciate the approach with making such lessons based on scientific articles, not outdated textbooks. Could you please leave references to such articles if possible?
Hello. I am glad the content is helpful. I can try to make a compilation of papers I have read overtime (which I think will take more time than I have at the moment) but I worry that the material simply may be overwhelming. This video alone was summarized through at least 10 or so papers - at some point I will get to making a list of my sources :) Thanks for the suggestion - I will try to implement this in my upcoming videos if possible :)
One of the best explaination i have seen yet on the topic... thank you v much sit 😊
Best explaining ever for this topic.
Very nice and neat. I suggest you add complementary video speaking about the two possible tracking mechanism suggested for Rho-helicase: Tether tracking and Rut-free tracking.
Will make a video :) Thanks for the suggestion!
You deserve more👌🔝
I appreciate your support :)
For the rRNA and tRNA do they use the intrinsic termination mechanism to undergo termination? As rRNA has a NUT site that blocks the Rho protein, let's say in intrinsic mechanism, the NUT site will be blocked outside of the transcription bubble by the Trigger loop, and the RNA: DNA hybrid is broken by the lid and rudder at the exit of RNA and DNA. Hope to get some feedback 🫶🏻
Did you explained the RNA polymerase proofreading mechanisms somewhere... If so please tell me.
BTW the videos are amazing, I learned the whole mRNA trasncription from here.. thanks to you I was able to prepare for my MGenetics Quiz.
Hello! I am glad the videos are useful. I have not yet made videos on proofreading - hopefully soon :)
Lecturers are so good can u plz suggest me a book 📖 where I can find all this.
What I didnt get just yet is how the haripin itself is formed. Are there any factors bending it or is it something else? Anyhow great video.
The hairpin forms because there is internal complementarity in the RNA/DNA - i.e. the definition of a hairpin. You don't need external factors to form a hairpin - maybe to stabilize it, but not to form it.
@@theCrux Ah nice, wasnt sure if the "attraction" to each other is enough on its own, thx
May b r rna and t rna termination occurs by rho independent mechanism
Yes, you are correct :)
Why doesn't nus factor prevent secondry structure formation at termination site during rho independent process since nus do it during elongation process...plz explain
NusG (typically) is the anti-terminator/elongation factor. But NusA is a termination factor. So not all Nus factors prevent secondary structures. Also, recent studies show that NusG can also bind to certain sites and stabilize secondary structures and thus, promoting secondary structure formation.
sir, thank you but can you open subtitles for all languages
Hello! The subtitle generation (in English) is done by UA-cam itself (I think long videos are difficult to auto-caption). Unfortunately, I do not have a written script for these videos. I apologize for the inconvenience.