How to decide pH of the mobile phase | HPLC mobile phase preparation | voice of kayani
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- Опубліковано 22 сер 2024
- How to decide pH of the mobile phase | HPLC mobile phase preparation | voice of kayani
This video based on HPLC mobile phase pH.
in this video explained how to decide pH for HPLC mobile phase?
how to set pH of the mobile phase discussed in this video.
in this video also explained the relationship between pH and pka value of the analyte.
Your quires;
HPLC
mobile phase
pH
mobile phase pH
pka
pka value
pH vs pka
how to decide pH for mobile phase.
HPLC troubleshooting
troubleshooting
pharmaceutical industry
chromatography
interview questions
QC Department interview questions
HPLC troubleshooting interview questions
voice of kayani
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Please make a Video on Related Substances Interview Questions
@@SAURAVKUMAR-zx2il ok dear,
Just keep watching and sharing
Hplc method development per video
Ok
Acidic pH ke liye mostly opa hi Q use kiya jata hai
HCl bhi use hota . Depending on mobile phase composition...
But phosphoric acid is having high acidity, having strong acidic nature,that's why used mostly
Sir API sampling , excipient( non API ) sampling ka rule same hai ? Aur guideline pls
@@vasudevsharma-ey6ni not same
@@voiceofkayani5419 to pls guideline bta dijiye ...../n +1 to API ke liye lagta hai na sir ..........
@@vasudevsharma-ey6ni yes /n +1 only for active substance
@vasudevsharma-ey6ni WHO guidelines
Sir tablet bland sample kitna lena chahiye
As per standard concentration to make same ppm solution with standard... should be in limit of detection
Sir ye impurity factor kya hota hai ?
@@vasudevsharma-ey6ni related to impurities calculation
@@voiceofkayani5419 kaise ? Iski importance , use
@vasudevsharma-ey6ni dear,
Pharmaceutical raw material Mai Jo impurities hoti Hain unka calculate Karna k impurity factor se related hai....iski bhot importance hai. Ye pharmacopeial hai material ki pure hona daikhna hota hai...
Inko related substance bhi kehtai Hain....
Is pe Meri videos available Hain plz watch on my channel
@@voiceofkayani5419 sir impurity use karne se phle kya validation hota hai uska ?