Yes, long read sequencing can be used for a more comprehensive view of the knock-in region of interest. Since the read length is longer, it can give a more complete spectrum of mutations induced by CRISPR-Cas9.
CRISPRi utilizes dCas9 with or without fused repressor domains (e.g. KRAB) along with a sgRNA to target the promoter regions for transcriptional repression or knockdown of a gene. Visit our knowledge base today to learn more: info.abmgood.com/crispr-cas9-gene-regulation-dCas9
Isn’t serial dilution a tedious way to isolate dna?
Can we use nanopore long-read sequencing for my CRISPR knock-in experiment validation?
Yes, long read sequencing can be used for a more comprehensive view of the knock-in region of interest. Since the read length is longer, it can give a more complete spectrum of mutations induced by CRISPR-Cas9.
Amazing Video!! Thank you :-)
Glad to hear that you found the video helpful! :) Thanks for watching!
What would you use for CRISPRi?
CRISPRi utilizes dCas9 with or without fused repressor domains (e.g. KRAB) along with a sgRNA to target the promoter regions for transcriptional repression or knockdown of a gene. Visit our knowledge base today to learn more: info.abmgood.com/crispr-cas9-gene-regulation-dCas9
woaw thankssss
Subtitle to spanish?
The days of Noah are here.
Not clear :/
Hello,
Please contact us at technical@abmgood.com if you have anything unclear! We will be more than happy to help you :)