Are you able to paste a section of the bed file here? I have never seen the situation you describe. What reference genome are you using for your experiment?
@@bioinformatics1015 chr10 0 73600 chr10 73600 73800 chr10 73800 74400 chr10 74400 76000 So in the first line,. that's really from chr10:0-736 bp, line 2 is chr10:736-738bp, and so on. Why does it end in '00'? Thanks!
@@quililank Since I am not sure how the bed was created, I would assume the extra '00's are a mistake. If you are certain it is supposed to be chr10:0-736, I would recommend stripping off the extra 0s in the file before use. Let me know if you have any more questions!
![[WEBINAR] Intro to Bioinformatics Pipelines for ChIP-Seq](http://i.ytimg.com/vi/BACMdx5X3yQ/mqdefault.jpg)
Superb! thank you
Please do more videos
is it possible to convert .tab output from HTSeq count to .bed file for visualization of coverage on igv?
I don't understand where the information about the sequence is tho?! Like where is the sequence?
Got a question, the exons you showed in the video as a box. Are those exons represent on ssDNA strand or dsDNA?
ds
hope you continue the channel man; useful content!
Thanks for the clear explanation!
Hi! Why does my start and end position end in'00'? Is this necessary?
Are you able to paste a section of the bed file here? I have never seen the situation you describe. What reference genome are you using for your experiment?
@@bioinformatics1015
chr10 0 73600
chr10 73600 73800
chr10 73800 74400
chr10 74400 76000
So in the first line,. that's really from chr10:0-736 bp, line 2 is chr10:736-738bp, and so on.
Why does it end in '00'? Thanks!
@@bioinformatics1015 I'm using hg38
@@quililank Since I am not sure how the bed was created, I would assume the extra '00's are a mistake. If you are certain it is supposed to be chr10:0-736, I would recommend stripping off the extra 0s in the file before use. Let me know if you have any more questions!