PyroFarms GrowPro Expansion Feeding (at night)
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- Опубліковано 25 чер 2021
- In this video we show how to expand your PyroDino culture by using the Expansion Feeding method. The Expansion Feeding method works best for three main reasons.
1) Feeds both the original culture and the new culture with one single feeding.
2) Prevents 'over dilution' during expansion which can stress the PyroDino population.
3) Mixing and combining PyroDino cultures is beneficial for the overall health of these dinoflagellates.
The Expansion Feeding method is simple.
1) Mix and aerate the DinoNutrients by shaking the pouch with the cap on tight.
2) Make sure the DinoNutrients are at the same temperature as the PyroDino cultures you are feeding.
3) Pour approximately equal volume (or less) into the primary culture, mix, and pour approximately the same volume added to the primary culture into the new expansion container.
4) Return both cultures to their daily lighted locations.
By using the Expansion Feeding method you can achieve large populations (several liters/gallons) of PyroDinos over a few weeks or a few months without over-diluting and stressing/killing your PyroDino culture.
PyroFarms sells DinoNutrients and Blue Boost DinoNutrients at PyroFarms.com and Amazon. For large-scale growing, bulk DinoNutrients and concentrated DinoNutrients can be purchased by custom order at PyroFarms.com (inquire through our website). We will also be posting a video on how to make your own saltwater growth media in a future video. So please subscribe.
Awesome🌟
Wow! This is so Amazon!
Yes it is Amazing and on Amazon
Putting it in a water fountain would be beautiful!
We have done this too. ua-cam.com/video/hrxC26s4oAI/v-deo.html
I have a question, how do you start this process? and what products do I need to get so I could start on the right foot?
We have several starter kits at PyroFarms.com. This video shows the GrowPro System which has everything you would need to get started. I recommend the GrowPro System to get started.
Does turning on lights it nightime affect their circadian rhythm and their glow, and what would happen when you add a dino nutrients to the mix as soon as they are out the bage in a glass.?
Usually turning on the lights at night will not affect the circadian cycle unless the lights are on for extended periods (hours) for several consecutive nights.
I just ordered a bag of Dinos with a bag of nutrients. With adequate maintenance feeding, how long will a culture last? Is it indefinite? When I run out of nutrients, will I need to order more to maintain my culture? (Yeah I know you want me to keep buying your stuff and I totally will, but if I use something like Instant Ocean salt instead, what happens? Does commercial reef tank salt have the same nutrients as what's in the bag I ordered?) And just out of sheer curiosity, if I stop feeding, will the culture slowly die back or does it eventually crash all at once?
Thanks! This is one of the coolest products I've ever seen and I am stupid excited!
A lot to unpack here. PyroDinos primarily require light and they can be grown (carried) for years. We have grown the same PyroDino culture for over 8 years now. We have grown PyroDino cultures for over a year with only daily light. Adding DinoNutrients adds space to grow, rebalances the salinity and adds optimal nutrients. You can use Instant Ocean if made correctly and sterilized. If you use Instant Ocean or reef salts I would recommend adding a small ratio of DinoNutrients (1:10 or 1:15) to add the required nutrients and to buffer the harsh artificial seawater. DinoNutrients is filtered purified Pacific Ocean seawater with added nutrients.
@@PyroFarms Thank you very much for the quick and thorough response!
@@PyroFarms One more question for you! My shipment got delayed several times. It was shipped on Monday and is still not here (Friday). How long can they survive with no light?
@@5ubv3rsion About 7 days is pushing the max time in the dark. Although we have had shipments go as long as 10 days in the dark.
@@PyroFarms Looks like they'll be here today so they should be okay. Thanks again!
How much sunlight should it get per day and how often do I feed it if I'm trying to grow into larger containers?
For optimal results use a light timer with a LED light or LED light strips. Bright white LED lights work best and colored LED ‘grow lights’ can also be used.
LUX: 1000 - 3000
Foot Candle (Fc): 90 - 280
PAR: 90 - 120 EU
example: single 800 lumen light (5000K) with reflector: 40cm - 80cm away
example: single 800 lumen light (5000K) w/o reflector: 15cm - 30cm away
Consistent daily lighting is best and will yield best results. A timer for 12 hours on (day cycle) and 12 hours off (night cycle) is a good starting point for a robust culture.
PyroFarms.com to learn more. pyrofarms.com/pages/grow-pro-instructions
@@PyroFarms thank you
So you can constantly add as long as you have clean containers…
Yes. As long as the container is clean and clear, like a mason jar or empty water bottle, you can populate with PyroDinos.
Do you guys at pyrofarms add CO2 to your cultures to grow or in bulk? I would like to expand and give friends and family for holiday gifts eventually so I’m going to grow in 5 gallon carboys and want to do it the most sterile and efficient way possible. Will they get enough CO2 from the filtered airline I will provide? Or do you somehow give them another carbon source? Thanks!
Good question. Excess airflow and supplemental CO2 is not required. If you grow in a carboy (about half filled) PyroDinos will have a good surface to air ratio which is all they need. We do not recommend adding bubbles. But do cover the carboy, not with a lid but, with an air-permeable membrane.
@@PyroFarms and I know expansion should happen when the culture is dense but was is the way you guys measure your culture density? Do you count a small amount of cells in a small volume and calculate it that way? Could you walk me through calculating cell density? A secchii disk doesn’t seem like a good idea because it could contaminate culture. Thanks
@@theaquaticsnailshop5668 We sometimes do cell counts under a stereoscope with hemocytometer but usually just monitor the growth (aggregation) around the meniscus (waterline). As the thin line of PyroDinos starts to thicken around the waterline we know it is time for feeding/expansion. If you do cell counts- look for cell numbers north of 120 cells/ml. This is the feeding zone..
@@PyroFarms Okay great, so once I see an accumulation of cells at the waterline of my two growpro flasks I can add more dynonutrients and expand? Also, once it’s time to expand would it be possible to add the same volume of dynonutrients that is already in the flask. For example, I have 100ml of culture in my Growpro flask, once it’s time to expand(water line dense with cells) can I add another 100ml of nutrients to flask for a total of 200ml and just repeat that process in the future?
Do you ever get contamination with bacteria, mold, or fungus?
This is possible if not taken care of properly.
@@PyroFarms can you give a hint as to the nature of the fungicide used in the blue boost food solution for the dinos? I'm trying to make a very large mix for an educational display. Is it copper based? I know you obviously want to maintain your product formula secrecy to protect your business from eg. cheap Chinese knockoffs and so I will delete this question later if you provide an answer.
@@Muonium1 Hi. Sorry but our Nutrient formulation is proprietary to PyroFarms. If you use aseptic technique** and keep your PyroDino culture dense and growing (do not over dilute with dinonutrients/sewater) you should not have a contamination problem. www.thermofisher.com/us/en/home/references/gibco-cell-culture-basics/aseptic-technique.html
**Note - Just the basics of aseptic technique are required, clean workspace, clean hands and sterile reagents (like DinoNutrients and Blue Boost).
What do you grow these things for...out of curiosity? Just to shake every now & then? Could you put them on one of those light stands that you put stones on and move? I don't understand their purpose. Or why. Not being a troll. I just saw the cool glow and watched the video. I happen to have 1 cockatiel, 2 South American Cichilds (lg Red Devil & lg Peacock) and 3 Labradors...one of each color. Sorry, if my pets shouldn't have been mentioned. 💞😊
Its a fair question. Think of a houseplant that lights up a night. These algae or plant plankton grow using daily light like a plant but this 'plant' lights up at night when you move it. So its like a plant-pet that you can take care of during the day (like a houseplant) and it provides natural light at night. And you can put this algae into your favorite glass vase or bottle. There is a whole other side about the air we breathe, the entire ocean food chain and climate change I can dive into but will refrain from right now. If you would like to learn more please visit our website pyrofarms.com/
@@PyroFarms Thanks.
If your thinking no way it can't be more simple than that your wrong it's that simple I just came back from that rabbit hole seriously more nutes and dim light it will do everything else itself.
Just keep containers clean.
So true. Some people try to overthink it.
I wish I had the shirt
name a price (~: