iMeta | Robust CRISPR/Mb2Cas12a genome editing tools in cotton plants

Robust CRISPR/Mb2Cas12a genome editing tools in cotton plants
Fengjiao Hui, Xu Tang, Bo Li, Muna Alariqi, Zhongping Xu, Qingying Meng, Yongxue Hu, Guanying Wang, Yong Zhang, Xianlong Zhang, Shuangxia Jin
First published: 04 June 2024
doi.org/10.100...
Graphical Abstract
The efficiency and accuracy of the CRISPR/Mb2Cas12a system were demonstrated in cotton, achieving an efficiency of over 90% at target sites. Notably, Mb2Cas12a exhibited significant tolerance under different temperatures ranging from 22°C to 32°C. Additionally, the Mb2Cas12a system revealed effective editing at more relaxed VTTV PAM sites in the cotton genome, which expanded the genome editing range by approximately 2.6-fold than the wide-type LbCas12a. Finally, a multiplex genome editing system was also developed based on Mb2Cas12a, enabling simultaneous editing of eight target sites using a single crRNA cassette.