Microbiology: Rapid Plasma Reagin (RPR) Test

This video describes the purpose, procedure, and result interpretation of the qualitative & semi-quantitative Non-Treponemal Screening (RPR) test for Reagin type antibodies. A comparison of this and the VDRL test is also included.

[@healthknowledgeexplorer]

Thanks ma'am, you are really doing an incredible job for us

[@MedicalLabLadyGill]

Thank you so much for your support!

[@-arabgamers3697]

Hello, I want to know why we used plasma in this test and did not use the serum ?

[@manoauu7293]

I'm from Iraq and I dream of being a good pharmasict my exam is after few days
The video was so helpful to me I'm so thankful ♥️

[@MedicalLabLadyGill]

Hope you did well!

[@jay-peeramos4573]

How to report the titer?

[@adriandc9761]

Please help. So I tested positive with rpr weak reaction titer 1;1.. Then did another test TPPA that one was non reactive. What should I make of these results? Thank you. Btw I am a male.

[@deeliaowens3166]

I have been looking at multiple images of card test like what you have in this video. How can I develop my ability to identify a positive on a patient barely showing a faint discoloration from reagen with a titer of 1:1? Images I have seen to have ratios of >1:8 would equal reactions quantified as 1:512. Several members of our lab are having extreme difficulty distinguishing positives such as this. I’m beginning to wonder if it’s realistic based on images I have seen available are very distinctive reactions which is not what we are seeing.

[@MedicalLabLadyGill]

Good question! There is more of a separation within the liquid itself when the test is positive. The aggregates end up spreading along the periphery of the test window to make a large ring.
Negative reactions tend to stay as a "fluid blob" or drop that mostly stays together as it rolls/flows along in the test window. You may see the particles of the reagent but that is not a true positive.

[@Khaledf]

Thank you. Why is it called non specific?

[@user-wr8oh1uu7h]

Apart from different type of process of this exam,just show us the interpretation of result on paper,thanks a lot.

[@prionzgaming5996]

Can post more videos like this on every test in the laboratory because im an intern and i dont have experiences during the pandemic and our school doesnt allow us to go to the hospital because of the virus

[@MedicalLabLadyGill]

Thank you for your support! I will do the best I can. Please stay well!

[@giar1274]

I’m here to refresh my memory of this test because it’s been a while and I want to be able to do this properly in a job 😭

[@tarisaimulenga9904]

💀 me too😭😭😭

[@MedicalLabLadyGill]

Thank you for your support! I hope you both found it helpful! Best wishes for your jobs ♡

[@zethdumalaog5358]

Can this this test detect STD?

[@pushpapuree990]

Ma'am plz tell me that can we use RPR for Borellia, acinetobactor also plz 🙏🙏🙏

[@DanielFernandez-fu7rv]

According to my microbiology books, RPR is usually positive for Borrelia burgdorferi and other bacteria, not only T. pallidum. About Acinectobacter, I don´t know sorry.

[@albertchesterton43]

Post more videos

[@amorprohibidooi5417]

RPR 1:1 results what's the meaning of

[@MedicalLabLadyGill]

That looks like a straight solution to me...just specimen. If there is a dilution, the person might have meant 1:2 (1 part specimen in 2 parts solution with an equal part being diluent).

[@VisceralCarbon]

Can this test also detect HIV?

[@MedicalLabLadyGill]

No

[@michaele.4702]

Great video though the spinning table made me dizzy.

[@MedicalLabLadyGill]

Sorry about that part! Glad you hung in there!

[@Narrative_hoook]

Strip test positive, rpr non reactive