Erratum: 13:36, the AGGAGG (Shine-Dalgarno sequence) is on the mRNA, whereas the UCCUCC is on the 16s rRNA. Somehow, I managed to put them the other way around 😢 8:30, IF1 recruits IF2-GTP to the A site - the video incorrectly depicts that IF2-GTP recruits IF1. IF1 binds the 30S first and changes a bit of 30S conformation to allow IF2-GTP positioning.
This is extremely detailed and clear, thank you for creating this and the other videos on your channels. As a chemist, I've noticed two details: - you keep naming complementary sequences in the 3'-5' direction, instead of using standard 5'-3' direction; for example the anticodone of AUG is CAU in the standard order, because the coupling is anti-parallel, but you say UAC. I understand that this is for educational clarity, but at times is not immediately obvious which of the two complementary sequences is named in the standard 5'-3' direction. - at 16:00, the way you note the hydrolysis of GTP attached to IF2 contains a contradiction. You can't hydrolise GTP into GDP and PPi (=inorganic pyrophosphate), because this conjures a phosphorus atom out of nowhere: it's either GMP and PPi or GDP and Pi (inorganic orthophosphate). Which is the case? I hope this is helpful.
Thank you so much for this amazing explanation. It's very clear and to the point, with all the necessary details. It's hard for me to understand the process without visualizing what's happening, and your drawings help a lot. You're such a great teacher of complicated molecular processes!
You might wanna recheck the binding action of initiation factors. IF1 binds to the A site and IF2 with GTP interacts with IF1, the small subunit and initiator trna.
Please see 10:21 There are many specific details that I could have gone into about tRNA positioning... but that seemed like too much information to include in this video.
Erratum:
13:36, the AGGAGG (Shine-Dalgarno sequence) is on the mRNA, whereas the UCCUCC is on the 16s rRNA. Somehow, I managed to put them the other way around 😢
8:30, IF1 recruits IF2-GTP to the A site - the video incorrectly depicts that IF2-GTP recruits IF1. IF1 binds the 30S first and changes a bit of 30S conformation to allow IF2-GTP positioning.
This is extremely detailed and clear, thank you for creating this and the other videos on your channels.
As a chemist, I've noticed two details:
- you keep naming complementary sequences in the 3'-5' direction, instead of using standard 5'-3' direction; for example the anticodone of AUG is CAU in the standard order, because the coupling is anti-parallel, but you say UAC. I understand that this is for educational clarity, but at times is not immediately obvious which of the two complementary sequences is named in the standard 5'-3' direction.
- at 16:00, the way you note the hydrolysis of GTP attached to IF2 contains a contradiction. You can't hydrolise GTP into GDP and PPi (=inorganic pyrophosphate), because this conjures a phosphorus atom out of nowhere: it's either GMP and PPi or GDP and Pi (inorganic orthophosphate). Which is the case?
I hope this is helpful.
Thank you so much for this amazing explanation. It's very clear and to the point, with all the necessary details. It's hard for me to understand the process without visualizing what's happening, and your drawings help a lot. You're such a great teacher of complicated molecular processes!
Your videos help me in my admision test for PhD, thank you so much 💜
Your videos help me alot for preparation of my UG exam & inspires me to learn more about these topics.THANKS ALOT
Keep the good work Sir. Amazing job 👏
Loved it.. please upload full translation.. waiting for more such videos..
You might wanna recheck the binding action of initiation factors. IF1 binds to the A site and IF2 with GTP interacts with IF1, the small subunit and initiator trna.
Wow! Amazing error catch. I have updated the erratum comment on this video. Thank you!
Thank you - amazing work!
Thnk u sir for uploading the video soon...waiting for elongation and termination.....
Uploaded or not?
Please upload a eukaryotic replication video
Great Video...
Good One
Sir from which book you refer to
amazing job! but how the initiator tRNA positiend itself between the factors before the mRNA come into place?
Please see 10:21 There are many specific details that I could have gone into about tRNA positioning... but that seemed like too much information to include in this video.
Legend❤
Sir kya aap profesor h or aap brussels?
❤❤❤😢
Wer lernt noch für Brüser?