Can I ask a question : As we know the Anthrax toxins consist from 3 main proteins, One of them is Edema Factor ( EF) which is adenylate cyclase class2 , the genes for this class are known as cyaA, one of which is anthrax toxin. This cyaA is Bifunctional hemolysin/ adenylate cyclase, after cleaved in two proteins a calmodulin-sensitive adenylate cyclase (cyaA-ACD) and hemolysin , and this hemolysin is responsible for hemolysis of RBCs , So finally How B.anthrax does not produce hemolysis on blood agar in laboratory diagnosis?
Regarding the procedure for the antibiotic susceptibility test, would it still work if a spread plate technique was done opposed to using a swap to cover the HE plate?
Hello! Is it possible to adapt this method to see the effect of visible light (blue, red, ..) on bacterial growth/inhibition? If not, which other method can be utilised? Thankyou!
I would put the bacteria on different plates and expose each plate to one wavelength of light. You could then count the number of colonies growing after exposure to different light. Or you could grow bacteria in tubes of broth exposed to different wavelengths of light and then measure growth in the tubes using a spectrophotometer.
They are similar, but not quite the same. Longer explanation here: labmonk.com/microbiological-assay-of-antibiotics-using-cup-plate-method#:~:text=In%20the%20cup%20plate%20method,measured%20around%20the%20antibiotic%20disc.
Hi, is there a std method of testing effectiveness of a biostatic agent applied to a surface? I’ve synthesized several colloidal copper hydrosols (suspended copper nanoparticles) to see if spraying on PPE will increase its antimicrobial properties. I was thinking of spraying discs and placing them in a lawn culture, but the nanoparticles are not intended to diffuse through the agar but rather stay attached to the material (discs). Is there a simple “zone of inhibition” type test, or can I just spray the hydrosol directly on part of the agar and look for growth? Heeeeeeelp!
You could use paper discs dipped in your hydrosols to “touch” inoculated agar, then remove, leaving a layer of hydrosol behind. Do it at various concentrations and see if bacteria are inhibited. Can you just culture bacteria in 96-well plates with varying concentrations for hydrosols?
Biology Professor thanks for you reply. Here’s a quick synopsis of what I’ve done: ua-cam.com/video/3TWMc3K_PAg/v-deo.html (sorry 4 the bad jokes in advance). I’d like to verify whether copper treated fabrics inhibit microbial growth and for how long. Unfortunately I only understand the chemistry part. Any direction would be greatly appreciated.
Usually 37C, but sometimes even 30C. Here is a link to multiple examples from the American Society for Microbiology and sometimes different people use different temperatures - www.asm.org/index.php/kirby-bauer
See #7 on page 16 of the American Society for Microbiology’s protocol: asm.org/getattachment/2594ce26-bd44-47f6-8287-0657aa9185ad/Kirby-Bauer-Disk-DiffusionSusceptibility-Test-Protocol-pdf.pdf I know it’s counterintuitive, but recording 0 mM for no zone of inhibition is pretty standard. It’s the only thing I’ve ever seen.
Thank you, Professor. This is the third of your videos that my instructor has shared with us in micro lab this semester. I love your videos.
Thanks for telling me! :)
Wow! You have such an excellent teaching method. Goes straight into the head without creating any confusions.
Please keep uploading videos 💚
The explanation was easy to follow and very clear! Wish my professor explained it like that!
You make microbiology so much easier to understand. Thank you for this video!
Thank you for the compliment! 😊
Concise explanation with impressive personality.
Excellent young professor. Keep up the good work.
Very INEFFECTIVE sensitivity should be very effective sensitivity
Yes, guys we got it, it is written 'ineffective', but she said again and again EFFECTIVE. Move on with your lives.
I was confused too when she said it effectet
@@marcus00077 ok.
It took only 9 minutes to understand this after thoroughly reading the lab manual for hours♥️♥️
Glad I can help!
most beautiful and helpful teacher ❤
Thank you
You are wonderful, thank you for making all your videos.
Very helpful , thank you so much
Thanks for sharing
Thanks for the explanation luv
Can I ask a question :
As we know the Anthrax toxins consist from 3 main proteins, One of them is Edema Factor ( EF) which is adenylate cyclase class2 , the genes for this class are known as cyaA, one of which is anthrax toxin.
This cyaA is Bifunctional hemolysin/ adenylate cyclase, after cleaved in two proteins a calmodulin-sensitive adenylate cyclase (cyaA-ACD) and hemolysin , and this hemolysin is responsible for hemolysis of RBCs ,
So finally How B.anthrax does not produce hemolysis on blood agar in laboratory diagnosis?
Can nutrient agar be used in this test?
I’ve used LB and nutrient agar with my students. We call it a “modified disc diffusion assay” since it is not the standard protocol.
Thank you so much, you are real life saver!
Very clear explanation! Thank you! :-)
Thank you so much 🌹🌹🌹
Where it says very ineffective should have been very effective. spelling error I guess
Oops! Yes, you are right. It should be "very effective".
Regarding the procedure for the antibiotic susceptibility test, would it still work if a spread plate technique was done opposed to using a swap to cover the HE plate?
of course!
This really helped me
thank you so much! best explanation
nice. thank you!!
You are welcome! :)
Tq u so much madam.....
Please could you also cover the linear regression for MIC estimation, based on the halo results?
I will add it to my list! Thanks! For now, this might help: academic.oup.com/jac/article/61/6/1295/739753
Thank you so much! Now I understand!! ;)
Can u do more this is such plain language my book said impregnated paper disk. Why do they need to complicate learning. Dumb it down then build it up.
Hello! Is it possible to adapt this method to see the effect of visible light (blue, red, ..) on bacterial growth/inhibition? If not, which other method can be utilised? Thankyou!
I would put the bacteria on different plates and expose each plate to one wavelength of light. You could then count the number of colonies growing after exposure to different light. Or you could grow bacteria in tubes of broth exposed to different wavelengths of light and then measure growth in the tubes using a spectrophotometer.
Is this method also called cup plate or is that something different?
They are similar, but not quite the same. Longer explanation here: labmonk.com/microbiological-assay-of-antibiotics-using-cup-plate-method#:~:text=In%20the%20cup%20plate%20method,measured%20around%20the%20antibiotic%20disc.
Hi, is there a std method of testing effectiveness of a biostatic agent applied to a surface? I’ve synthesized several colloidal copper hydrosols (suspended copper nanoparticles) to see if spraying on PPE will increase its antimicrobial properties. I was thinking of spraying discs and placing them in a lawn culture, but the nanoparticles are not intended to diffuse through the agar but rather stay attached to the material (discs). Is there a simple “zone of inhibition” type test, or can I just spray the hydrosol directly on part of the agar and look for growth? Heeeeeeelp!
You could use paper discs dipped in your hydrosols to “touch” inoculated agar, then remove, leaving a layer of hydrosol behind. Do it at various concentrations and see if bacteria are inhibited. Can you just culture bacteria in 96-well plates with varying concentrations for hydrosols?
Biology Professor thanks for you reply. Here’s a quick synopsis of what I’ve done: ua-cam.com/video/3TWMc3K_PAg/v-deo.html (sorry 4 the bad jokes in advance). I’d like to verify whether copper treated fabrics inhibit microbial growth and for how long. Unfortunately I only understand the chemistry part. Any direction would be greatly appreciated.
very interesting
Can u please upload the video about microbial assay of streptomycin by cup plate method
I have added it to my list. Thanks for the suggestion
It was very helfpul ...love you maam
I think she was supposed to put very effective not very ineffective on the large shadow/zone
Yes, it was a mistake.
effective video
Tq mam
very effective is being written as ineffective.
Whoops! You are right. I say it correctly, but it is written incorrectly.
Thanks young pretty teacher!
Is it 37 c or 35 c
Usually 37C, but sometimes even 30C. Here is a link to multiple examples from the American Society for Microbiology and sometimes different people use different temperatures - www.asm.org/index.php/kirby-bauer
@@BiologyProfessor Thank you Professor.
Oh my gaad..you're eyes are so pretty and menacing at the same time...Is this love at first sight❤😍..Thanks for the i formation tho.😊
creep
@@colummccrudden101 naaah...im just trollin.
🤗
I am coming across the reporting of 0 mm diameter for the first time. I know of reporting
6 mm, which is the diameter of the disc.
See #7 on page 16 of the American Society for Microbiology’s protocol: asm.org/getattachment/2594ce26-bd44-47f6-8287-0657aa9185ad/Kirby-Bauer-Disk-DiffusionSusceptibility-Test-Protocol-pdf.pdf
I know it’s counterintuitive, but recording 0 mM for no zone of inhibition is pretty standard. It’s the only thing I’ve ever seen.
This can't be right...
aren't smart girls supposed to be ugly?
Lybo Manolov the more you use stereo types to navigate life ... the deeper you fall down the rabbits hole...
you see the world with a key hole