The video has explained the content in a very detailed manner! I wanna know, how can the duration of a particular phase is correlated with the number of cells?
I can't understand why the cell count drops so drastically. The last example that you presented, at G1 the count was 1100, then at S it was around 100, at G2 it was around 110, so cell count had decreased by 10 times. Why? Do explain. Thanks!
It is just an example, depending what type of cell and from which organism you are working on, you could see different peaks. In this example, these cells seem to spend more time in the G1 phase, so that's why we see such a peak at G1; however, there still are some cells at the S and G2 phase, which is the rest of the curve, but clearly not as much as in the G1 (this is because these cells seem to spend less time in these stages). I think that for the most part, cells follow this timeline, but some cells like those of yeast spend much more time in the G2 phase; if we were to work on them we would see a bigger peak in G2.
I keep observing that they begin with N or with 1C. Are you suggesting that gametes duplicate their genome? how is that possible? YOu mean from 2N to 4N
I have a q , why do all cells atthe begaining have the same amount of DNA? and then in S have diffrent? Isnt all the cells are independent from the first to the kast up to the cell condetion and age?! Please answer me
it might be because S phase is relatively long and that in this long phase, the cells are replicating their DNA at different speeds. G1 phase is also long, but they are not replicating, so the DNA content is consistent.
So far, the best lecture I found about Flow cytometry for cell cycle analysis. Thanks.
Glad you found it useful!
I have the same comment. Short, consize and right the point. Thanks
You explained that topic so well. Thank you so much!!
This is great! I have been searching for this information and I finally found it. Thank you!
You are welcome. I'm glad you found it useful.
Thanks in a million sir.
I have a question. If all DNA has the same negative charge, how would it then be able to sort for example aneuploidy cells from diploid cells?
Nice lecture..so I understand more about it, thank very much
Thank you very much! Really helped a lot...
This is incredible! Thanks a million :)
The video has explained the content in a very detailed manner! I wanna know, how can the duration of a particular phase is correlated with the number of cells?
I can't understand why the cell count drops so drastically. The last example that you presented, at G1 the count was 1100, then at S it was around 100, at G2 it was around 110, so cell count had decreased by 10 times. Why? Do explain. Thanks!
It is just an example, depending what type of cell and from which organism you are working on, you could see different peaks. In this example, these cells seem to spend more time in the G1 phase, so that's why we see such a peak at G1; however, there still are some cells at the S and G2 phase, which is the rest of the curve, but clearly not as much as in the G1 (this is because these cells seem to spend less time in these stages). I think that for the most part, cells follow this timeline, but some cells like those of yeast spend much more time in the G2 phase; if we were to work on them we would see a bigger peak in G2.
Thank you so much. It helped me alot
Many thanks. Please, my cell cycle statistics is more than 100%. Why, please?
Thank you so much!
thank u so much it is very useful, simple and deep (Y)
Simple but very effective lecture... Thanks. :)
great ! Thank you
In a graph it is mentioned that no. of cells vary from G1>S>G2, represented in Y-axis. Please explain this aspect...
it is a tally of cells in each phase within a population of asynchronous cells. e.g. most cells in a population will be in G0/G1 part of the cycle.
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clear ，thanks！
thank you sir
I keep observing that they begin with N or with 1C. Are you suggesting that gametes duplicate their genome? how is that possible? YOu mean from 2N to 4N
I have a q , why do all cells atthe begaining have the same amount of DNA? and then in S have diffrent? Isnt all the cells are independent from the first to the kast up to the cell condetion and age?! Please answer me
Im just a new in this faild and i amost have lack of infos
it might be because S phase is relatively long and that in this long phase, the cells are replicating their DNA at different speeds. G1 phase is also long, but they are not replicating, so the DNA content is consistent.
Manners where are you thank u