Great stuff. Hand-held cutting with a new razor blade under a 20x (2x objective + 10x ocular ) stereomicroscope can also give exceptionally good results with some training. This was always the best solution for me, I preferred over a hand-held microtome.
New idea using clear glue. I have an extensive collection of pollen (my special study). I use gelatin sealed with varnish rings around the covers lips. Would Elmer’s work instead.
Thank you so much, another interesting and educational live stream, I don’t have a 3d printer but would like to use razor blades with my microtome, do you know of anywhere I can buy something like the plastic blade holder you made?
I watched someone cutting stem segments freehand using a razor, and it seemed much less of a hassle than the microtome. What is you opinion on this, and do you have a preference?
outstanding detailed instructional video. Thank you! great tip with the Elmer's glue mounting medium
Have you ever tried food colouring in place of Methylene blue?
Do you think that it works?
Great stuff. Hand-held cutting with a new razor blade under a 20x (2x objective + 10x ocular ) stereomicroscope can also give exceptionally good results with some training. This was always the best solution for me, I preferred over a hand-held microtome.
this are no cell-membranes by plasmolysis. This are simply the cell-walls which you cutted
I thougt the same
I like it so much
Amazing .....
New idea using clear glue. I have an extensive collection of pollen (my special study). I use gelatin sealed with varnish rings around the covers lips. Would Elmer’s work instead.
Thank you so much, another interesting and educational live stream, I don’t have a 3d printer but would like to use razor blades with my microtome, do you know of anywhere I can buy something like the plastic blade holder you made?
Morning
Hi Oliver! Have you ever considered disassembling maybe a spare or broken objective and putting it under the microscope?
Yes, but I did not put it under the microscope. I took one apart. Check this out: ua-cam.com/video/OiRxYpvfMIw/v-deo.html
I watched someone cutting stem segments freehand using a razor, and it seemed much less of a hassle than the microtome. What is you opinion on this, and do you have a preference?
totaly overstained, no clearing