Choosing a mounting medium for microscopy

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  • Опубліковано 25 лис 2024

КОМЕНТАРІ • 12

  • @garygraham6020
    @garygraham6020 9 місяців тому +4

    I use a formula for pollen mounts, it is a combination of glycerin, gelatin and Listerine mouthwash (prevents mold in the jelly). I melt on the slide with a warming table, after adding the pollen and cover slip, the gelatin becomes semi-sold.

  • @Mitch.Wasserman
    @Mitch.Wasserman 11 місяців тому +2

    Oliver, you have inspired me in many ways re: microscopy and this time you energized me to create a DIY slide ringing device. I used 2 CD disks and mounted on a large bolt and inserted into a hand drill to make it spin. Works great. I tried uploading a photo to the forum but was unable to post the photos.

  • @1rayemery
    @1rayemery 11 місяців тому +3

    Many, many thanks for all of your efforts this past year - entertainment with information! Have a good rest over Christmas! Best wishes to you & yours!

  • @mothman_slayer4217
    @mothman_slayer4217 4 дні тому

    What permanent mounting medium would be best for parasitology like ova, nematodes, ect?
    Along with WBCs?

  • @anonymusanonymus3343
    @anonymusanonymus3343 11 місяців тому +1

    @Microbehunter, the video is hidden, it doesn't show up in the channel, I don't know if you have hidden it on purpose.

    • @Microbehunter
      @Microbehunter  11 місяців тому +2

      Hello, the video will be online in a few hours. UA-cam is still processing it.

  • @bittersweet82
    @bittersweet82 11 місяців тому +1

    Hi there,
    May I ask where you got your slide spinner from, the electric ones are quite pricey.
    Also, when using nail varnish as a mounting agent, can you mount a coverslip over a dry specimen using the circle technique? Or do you still need to add something on top of specimen.

    • @Microbehunter
      @Microbehunter  11 місяців тому +1

      Slide ringing table (scroll down): www.brunelmicroscopessecure.co.uk/acatalog/universal.html
      You can make a "dry mount" without mounting medium over the specimen. But you still need to make a circle first (1) on the blank slide, then you add the specimen, then the cover glass, and then you make another circle (2) to glue the cover glass on circle (1). If you do not make circle (1) first, then the nail polish will go under the cover glass when you put on circle (2). If circle (1) is too dry then the cover glass will not hold when you put it on. in this case add another circle over (1) to make the cover glass stick.

    • @bittersweet82
      @bittersweet82 11 місяців тому

      @@Microbehunter Thank you!

  • @Francois_Dupont
    @Francois_Dupont 11 місяців тому +1

    i have a question for knowledgeable people, if somebody could please help me.
    i love been in the woods and i have a couple of Sawyer water filters. i recently saw a rare video of somebody testing a couple different water filter model and according to his results many were clearly defective. i am shocked and i am searching around for an easy way to test those.
    somebody has an easy~ way to test potable water for bacteria? ive seen tests for sale online, but according to the reviews they dont seem reliable and a waste of money. should i just buy a microscope and "manually" check the water sample by eye? maybe try the petri dish method and make cultures?

    • @Microbehunter
      @Microbehunter  11 місяців тому +1

      A microscope is not going to help. Bacteria may be present but at a too low concentration to see them. You would have to culture them to do a colony count. Normally these tests should have been done by the company who sell the filters. Also the presence of bacteria alone is not the issue. It depends on the type of bacteria present (ie fecal bacteria) and different kinds can not be distinguished with a microscope anyway.

    • @Francois_Dupont
      @Francois_Dupont 11 місяців тому

      @@Microbehunter i see. Thank you very much for your expertise. i was right to consult we a knowledgeable person before. you saved my alot of time and money with your advice.
      i wish you Happy Christmas!