Clinical Chemistry 1 Immunoassays

An overview of immunoassays in clinical chemistry, based on Larson's Clinical Chemistry, chapter 4 on immunoassays. Basic principles of immunoassays, heterogenous vs homogenous, competitive vs non-competitive, and various specific immunoassays. Calibration, quality control and the hook effect.
Videos links: • Enzyme immunoassay (EI...
• How Electrochemilumine...
• Fluorescence Polarization
PPT Handout: drive.google.com/file/d/16kO8...
0:00 Introduction
0:21 Antibodies, Antigens, and Analytes
2:28 Basics of Immunochemical Reactions
5:39 Antibodies Are Immunoglobulins
6:54 Affinity and Avidity
9:04 Heterogeneous Immunoassays
10:14 Homogeneous Immunoassays
15:18 Noncompetitive Immunoassays
17:04 Immunoassay Labels
19:25 Enzyme-Linked Immunosorbent Assay
24:28 Enzyme-Multiplied Immunoassay Technology
27:37 Fluorescence Polarization
30:47 Microparticle Enzyme Immunoassay
32:42 Chemiluminescent Microparticle
35:37 Cloned Enzyme Donor Immunoassay
37:23 Particle Methods
38:05 Immunodiffusion
40:27 Immunoelectrophoresis: Western blot
42:12 Light-Scattering Methods
43:21 Factors Affecting Immunoassay Performance
45:43 Hook Effect

[@dollparade]

Thank you so much for this video! It really helped explain things more simply on this topic that I was struggling with!

[@DrAsClinicalLabVideos]

Glad it was helpful!

[@justingray7290]

Thanks to you I won’t fail tomorrow
Thankyou so much

[@aseelasool3289]

Thank u❤

[@kateina5]

nice video to watch

[@DrAsClinicalLabVideos]

Thanks

[@KhadieJolie]

Can you please share the answer to the question about the control that was above the mean value?

[@DrAsClinicalLabVideos]

the answer is ...improper washing technique!

[@tausifraza6910]

I am unable to find the videos that she mentioned during in her presentation. Very good presentation!

[@DrAsClinicalLabVideos]

The URL addresses are in the video description; but there could be an access issue due to geographic location.

[@tausifraza6910]

@@DrAsClinicalLabVideos Is it possible to provide the links in the reply ?

[@tausifraza6910]

@@DrAsClinicalLabVideos Hi, What is the difference between clinical chemistry and immunoassay?

[@DrAsClinicalLabVideos]

@@tausifraza6910 Videos links: ua-cam.com/video/70TPrfL_8-M/v-deo.html​
ua-cam.com/video/PHbcvBGfzMM/v-deo.html​
ua-cam.com/video/OdBNVrPvJMY/v-deo.html

[@DrAsClinicalLabVideos]

@@tausifraza6910 Immunoassay techniques are often used in clinical chemistry to measure drugs, hormones and certain proteins. They are usually an integral part of large clinical chemistry analyzers. However, not all immunoassays are clinical chemistry tests, some are used in microbiology and immunology/serology.