Apigenin Increases Muscle Mass And Improves Muscle Function In Both Young And Old Mice
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- Опубліковано 3 жов 2024
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Video link, NAD+ is increased in response to apigenin:
• Are there foods that c...
Paper links:
Apigenin enhances skeletal muscle hypertrophy and myoblast differentiation by regulating Prmt7:
pubmed.ncbi.nl...
Apigenin Ameliorates the Obesity‐Induced Skeletal Muscle Atrophy by Attenuating Mitochondrial Dysfunction in the Muscle of Obese Mice:
onlinelibrary....
Antioxidant Apigenin Relieves Age-Related Muscle Atrophy by Inhibiting Oxidative Stress and Hyperactive Mitophagy and Apoptosis in Skeletal Muscle of Mice:
pubmed.ncbi.nl...
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These clinical dosages are huge! Big props for including real scientific literature though. Most supplements on the market are around 50-200mg
Looking forward to the results of your n(1) study! Thank you for putting this together.
Great news for the mice!
Yes, that'd be a heck of a lot of parsley and chamomile tea to have! Would be interesting to do this study on female mice and control for the menstrual cycle.
Nice video, always nice to find others with a similar subject direction for their channel.
Great video as always!
very good, solid information! well done!
Thanks @Sun-lq6od!
thanx for the dosage info. i hope to start taking 20-1 extract soon. i also see 100-1 available. double benefits of muscle building and sleep...just what i need.
Thanks for putting in the table to convert from animal species dosage to human dosages. I've never seen that before. And thanks for calling out the limitation of these studies that exclude females!! SO tired of this. Happens in human research too, way too often. (You should edit your video description to say "young and old male mice"). In the third study, it's interesting that the highest dose seemed to be less beneficial than the lower dose.
Thanks Amy. I try my best to be objective and without bias, which means that I call out any BS, wherever I see it!
Ha, in terms of changing the description, any women seeing that it doesn't include data for females may not watch, and I think the info in the video is important for all to see, not just men.
I'm not surprised about dosage, there are U-shaped curves for many aspects of biochemistry.
Great, informative video. Thank you!
Very informative thanks you
Very interesting. Thanks!
Wow amazing content
Based on this I am using lots of dried parsley on my food.
I prefer fresh, but no worries!
It's a really cool test!
Great video and channel, subscribed. Thank you
Thanks David P, and welcome!
Thank you!
You might consider doing a segment on Ecdysterone (found in mushrooms, quinoa, and spinach). It's getting a lot of attention in the bodybuilding world, but I don't know that anyone is looking at it in the health span space. There may be too few studies though.
Thanks jack buaer, I'll look into ecdysterone, and video ideas are always welcome!
So 288mg, dried parsley 6 grams.
That's one way to do it!
great work thanks
So, 6.4 grams a day of dried parsley would hit the 288 mg mark?
Yep!
@@conqueragingordietrying123 I also think about the fact that parsley thins the blood. This is something to consider with parsley. In high doses, could parsley then be a potential danger to heart health? What would be your advice on this? Thank you!
@@59250em If that were true, we'd expect to see a higher mortality risk in people who eat more green leafy vegetables. When considering the Vitamin K1 data (which is found almost exclusively in green leafy veg, that's not the case:
ua-cam.com/video/znX-fEF9YlY/v-deo.html
@@conqueragingordietrying123 Thank you very much for your answer.
Huberman recommends 50 mg for improved sleep
There is no human RCT data that supports that recommendation, but if it works for you, go for it!
don't forget that CD38 has an important role in the immune system... it link to LPS is obvious. Blocking the unspecific response of the immune system may not be a good idea in some contexts. Hence, did you relate your intake to LPS? Do you track serum LPS? in relation to your soluble fibers, and acetate, acid intake?
I have CD38 videos on this channel, I was probably one of the 1st channels to raise the CD38 issue on YT...
I haven't measured LPS, but if LPS is elevated, I'd expect increased inflammation. In terms of hsCRP, that's not true in my data.
If they let the mice eat freely they both get fat and a varying dose.
I think they should feed them so they both stay matabolically healthy and also get the same dose.
I use dried parsley, however I am not yet sure how to take it. What I usually do I grind it to the fine powder. I can add the powder different types of meals however since apigenin can make you sleepy I am considering how to take it before sleep, but make it easy for the digestion so I can take it closer to the bed time.
Have you considered to combine it with olive oil?
Great Video!
Thanks Manuel Ojeda!
Hi, a bit of topic, but have you ever heard of Phase Angle (PhA) which is a measurement of cell membranes integrity, hydration and nutrition status. Have you ever checked what your score is?
I haven't. Got a link, or a Pubmed reference?
Do you think parsley tea can help here?
imo, including some parsley, whether in tea or food may have benefit, relative to no parsley. How much is optimal in people, though, is debatable.
S9, im a 58 year old female. I loft weights, heavy weight, im 5'3" and weight about 115. How much should I take of apigenin?
Anyone lose a bunch of weight taking Epigenin? I took supplement form. I was on this and speridine not sure whitch or if it was both that caused it. But either way stoked i got pretty ripped. Curious if anyone else experience the same weight loss please post if your experience thanks.
Love your channel … had to give up on parsley … 1 tbsp upset my stomach .. no way I could do 5 … broke down and got the supplement.
Thanks @jp7357. Have you tried fresh parsley?
Dried Parsley has a lot of oxalate I think that may cause kidney stones?
Why not use fresh parsley, then? Also, the oxalate story is more complicated than just oxalate intake-the gut microbiome and kidney function are involved, too.
@@conqueragingordietrying123 Thanks for extra info. I use Apigenin capsules now 500 gr a day + moderate dried parsley + some fresh parsley at breakfast to cover all basis with natural approach.
@@wrecklessg6710 Five foods have been documented to increase urinary oxalate:
-Nuts (peanuts and pecans)
-Wheat bran
-Spinach
-Beets (root and greens)
-Chocolate (dark)
The bioavailability of oxalates in parsley is very low, so you're likely safe.
Some say we need 30gm of dried parsely to get the required dose.. not sure of their calculations
Nobody knows the exact dose that will be beneficial in people. With that in mind, I eat 50g of fresh parsley/d with the thought that some apigenin is better than none.
Thanks for the video. How about if I juice Parsley ? Do I get less apigenin? I normally juice parsley, cilantro, mint, ginger, lemon and celery in the morning. Thanks
You'll definitely get apigenin if you juice parsley, but you lose the fiber, which is essential for optimizing gut health.
Thanks. Any idea about mechanism of action? NAD+:NADH increase via CD38 decrease possibly ?
Yes, that would be the most likely explanation. Unfortunately, none of these papers quantified NAD...
@@conqueragingordietrying123 if this mechanism is confirmed, would sulphurophane still a better candidate for the same effects ? thx for your work
@@Ahrimanfr Good question, I have a sulforaphane post on my website, but I may do a deeper dive to see if it's as good for affecting muscle.
This is a little bit offtopic, but I was wondering: Is the excel spreadsheet you use to track your diet available somewhere online? I didn't find anything like that, but it'd be easier to use someone elses than building my own
No worries-I use a simple Excel spreadsheet (with many tabs), and then input all my dietary data a few times per week. I also look for correlations in that data with the blood test results. Anyone can do it, there's no special chart or file, with the exception of the biological age calculator that I use.
@@conqueragingordietrying123 oh, yeah I know how to set up the spreadsheet in theory. I was just wondering if I could download an empty version of the spreadsheet so that I don't have to set it up (probably saves me a an hour at least). I'd even pay for it. But yeah, if not then that's okay too and I'll set up my own!
@@conqueragingordietrying123 Hello Dr! I is amazing to see the work you do on self-experimentation and documentation... on the same note would you share the Excel Spreadsheet with the calculations to start doing some monitoring myself? Thank you for the work and videos you share! Maybe upload into Google Docs for easy access?
@@finukai The Excel sheet started off blank. Then, every day I enter all the macro- and micro-nutrient data, and individual food intake in different columns. When I blood test, I put that in a separate tab, and look for correlations between my dietary intake with the biomarkers. So you can do that for your own data, if you like!
Thanks for the video ! Like!!
I've got some fresh parsley now, roughly what's the average of apigenin in 15g dried parsley?
That data is in the video, starting at 12:14
@@conqueragingordietrying123 Ok thanks !
@@conqueragingordietrying123 What time would you say is the best time to eat the parsley powder?
@@moiLawUk Some is probably better than none, but the when is totally unknown
@@conqueragingordietrying123 Ok thanks my powdered leaf is mostly green with some yellow so probably not the freshest !
So how long did the mice live?
Is it possible to get a blood test for NAD+ and CD38 levels?
NAD+, yes, but I haven't seen CD38 available commercially.
I make my own capsules using dried parsley 00 size capsules how many would you estimate daily for this
That's an interesting approach. Many capsules come in 1g amounts, so 6 would get you close to 288 mg of apigenin/day, which increased muscle mass and function in the 3rd study in the video.
@@conqueragingordietrying123 my schedule is so busy with my job I barely have enough time to make these and take them that’s why I do it this way fast and easy
How much ch apigenin in parsley tea?
Maybe I'm not understanding some of this, but i feel like the math is off on some things. If the tea is at most 91mg/500ml and lowest concentration is 23.5/500ml, then the amount you need for the 23.5 should be almost 4 x the 91 amount and a total of 3.2L, i don't know where the 6000L comes from?
Also if you need 50mg/kg, then a 71kg person needs approximately ~3550mg, not 288? What am i reading wrong?
The problem with the apigenin in Chamomile tea is that it ranges (in different studies) from 1 - 91 mg/500 mL. To get to 288 mg/d with the highest dose (91 mg/0.5L), you'd need ~3x, which is the 1.6L estimation.
The 6000 L is a mistake, good catch! In the video, I mentioned the median value (1 to 23.5), 12 mg/500 mL, which translates into 12L, not 6000L. Even with 1mg of apigenin/500 mL, that's 144L, not 6000L.
For 50 mg/kg, that was in mice, and the body surface conversion is 12.3, so divide 50 by 12.3. Then, it's 4.06 mg/kg body weight, and multiplied by a 71kg person yields 288 mg.
Is there apigenin in frozen parsley?
Yes
How do you eat a lot of parsley without getting sick of it? Put it in soup?
I eat ~20g of parsley/d, that's my approximate threshold based on taste. Some can eat volumes of it dried, though.
@@conqueragingordietrying123 dried it is pretty tasteless and easy to eat (in water). Get that evening camomile tea in as well.
@@1980sDude As I mentioned at the end of the video, chamomile tea has large variability for apigenin content, from as little as 1mg/500 mL tea to 91 mg/500 mL. Parsley is the better choice for apigenin, either dried or fresh.
@@conqueragingordietrying123 Thanks. Luckily I've been relying on Parsley, but I'll have to up the dose after watching this. Great info.
I put dried parsley in my smoothie every day. The fruit overwhelms the taste of the parsley along with the other greens I add in.
Is low carb or high carb better? (From healthy sources of course). What is ideal carb intake
Based on the apigenin data, it looks like it doesn't matter if it's low carb or high carb.
Hi I've been taking 15g of dried parsley for a week now not noticed difference in muscles or libido.whst time is best time to take it and how? I take it with food or mixed with kefir ...
Thanks
It works in mice, but it's totally unknown if it will work in people. That said, based on the studies in the video, some may be better than none-in support of that idea, I include an average of ~50g of fresh parsley every day.
@@conqueragingordietrying123 Ok so 2nd week of parsley ,I have been feeling slightly agitated and have noticed weight strength increases at the gym. now I restarted the gym properly 6 weeks ago after a arm injury for 2months so whether this is natural or if this is the parsley or the NMN- who knows lol? I am 40 yrs old btw
@@moiLawUk Did you continue with the parsley? Or do any experiments with dosage? I'd be curious what your results were
Is parsley extract efficatious, and how much do you need? I have tried to google this, but I can't find it.
The data for parsley is in the video...
@@conqueragingordietrying123 Ok, is that the same as Parsley extract? Would that be better?
@@LaserGuidedLoogie I prefer to get apigenin from food, that's lowest risk, but if people choose to supplement with apigenin, that's a decision up to them.
@@conqueragingordietrying123 Thanks for your replies. The reason why I'm asking is that I find eating a bunch of parsley every day to be a chore, but It's easy to add a small scoop or 2 of parsley extract to my coffee. The parsley extract I use comes in a bag and is dry, and light green in color. I was under the impression that this was "food," am I wrong in that?
@@LaserGuidedLoogie It is food, but nutrient density is maximal in the whole (not dried food). I prefer to get it fresh, but others can decide what's best for them...
So how many tablespoons of dried parsley is recommended, approximately?
20 grams is what he takes apparently
@@LTPottenger How many tablespoons is that? Or partial cup?
@@lisellesloan3191 Taking the upper values of dried parsley at ~ 137mg/g (www.ncbi.nlm.nih.gov/pmc/articles/PMC4942635/), you need around 4g dried parsley a day (I'm 80kg), which is about two heaped tablespoons. It goes pretty well in a fruit or veggie smoothie.
I buy organic parsley spice at Walmart a about $4 per 7 gram bottle. Use a little for just cooking. If need 4 grams per day for the apiginin benefit will get expensive.
@@michael-qp9xd buy the big container of dried parsley. Much cheaper
I wish we did not need such a big dose
It's possible that some is better than none, that's my perspective. We'll all drive ourself crazy trying to find the exact human-equivalent dose.
You say "uh" at least once per sentence.
Yes, sorry about that, but the good news is that I've dramatically reduced it since earlier videos.
Why do people go through all the work of doing research like this without accounting for variables such as dosage?