Alex: buy a cheap CPL filter (circular polarisation filter) for your lens. With this filter you can reduce the appearance of reflections on glass for your video by rotating them to filter out the polarised light that makes up the reflection. They work miracles and you can find them at any photography store. There is very little quality difference between cheap and expensive variants. You can check whether they work by aiming them at a LCD display and rotating them. LCD displays send out highly polarised light, so a true CPL filter will show a black screen when you rotate the filter correctly. Considering you work with glassware a lot, this can certainly improve your videos!
You even get them on Amazon for a couple bucks, no need to waste time driving to a store. But they should fit for the camera model that's used - if the camera lens even has a threaded ring to put a filter or something else on there...
I love this concept, hope to see more great Lab Next Door videos in the future. By the way, that shirt looks really nice on you. Your videos always make my day a little better, thanks!
Oh I wish there were videos like that not only about bio-technology, but also aboout things like how someone is developing a better polymer for an organic light emitting diode (or an OLED display), or how someone is developing a new and better chemnistry for rechargeable batteries or things like that. This would be much more "up my alley" than all that bio-stuff, LOL ;)
Those are probably more locked away behind secret doors to allow companies and researchers to patent their discoveries and profit from them (or at least the University might). If they showed stuff while still researching, someone could beat them to the punch at the USPTO or elsewhere (like Europe or China).
Daemonstorm Animations You might be right about that. Although, if it's still some kind of basic research, it couldn't be patented, because there is no actual product as a result. Also, what I mentioned were just examples...
i would have loved to see some actual data. I have never seen this kind of assay, but it seems like a really cool and sophisticated technique. Only with what you showed us I can't imagine how to evaluate and draw conclusions from these kind of experiments
1) Why do they call it aspiration? Because that is the specific technique she's using to take the liquid off it. There are many ways to get rid of the liquid. She could pour it off, paper towel it off, shake it off... but each of those methods have their own end results for the thing in question. So, she was told to aspirate it off. 2) proteins aren't coated in hydrophilic coatings. They are composed of various amino acids and fold based on what those amino acids are willing to be around to reduce their internal energy and therefore the overall stress on the protein strand. So, when in water (which proteins tend to be as organic beings are bags of mostly water, heheh) they fold such that the hydrophobic regions cluster near each other to exclude water from being near them while the hydrophilic areas fold to face out towards the water. 3) polymerization isn't about turning something from a liquid into a solid. It is about growing things from monomeric bases to either long strands or networks of strands. Proteins are polymers composed of amino acids that are covalently bonded to each other. Hydrogels are able to absorb water into their internal structure as the various strands connect to each other (usually be simple hydrogen bonding and not by covalent or ionic bonds) creating a network of twisted up fibers that trap the water inside. This is why it is soft and squishy and deforms under pressure as it isn't an actual solid.
You might want to experiment with a polarizing filter for the camera to cut down on the glare and/or increase the color saturation which can make for a prettier picture. But you'd probably mostly use it for cutting out glare.
Oh, I thought the Yoda picture was a poster of inspiration. I believe he is delivering the "Do or do not there is no try" line during that frame, (well not during the frame but you get what I mean.)
"Do or do not there is no try" :) Would it be possible to get the results of Sasha's experiment? Thanks, I am looking forward to more videos from this series. :)
They are studying the forces applied by cells within a gel. ie: if you put a heart muscle cell here (or there), how powerful is it, how far away can it push things, etc.
I Really excited to see more of these types of videos :D
I'm so glad! I'm excited to make more!
Awesome video! The way you interleaved the step-by-step was so perfect. Great job!
Very interesting! I love the glimpse you're giving us into the real world of biology research.
Alex: buy a cheap CPL filter (circular polarisation filter) for your lens. With this filter you can reduce the appearance of reflections on glass for your video by rotating them to filter out the polarised light that makes up the reflection.
They work miracles and you can find them at any photography store. There is very little quality difference between cheap and expensive variants. You can check whether they work by aiming them at a LCD display and rotating them. LCD displays send out highly polarised light, so a true CPL filter will show a black screen when you rotate the filter correctly.
Considering you work with glassware a lot, this can certainly improve your videos!
You even get them on Amazon for a couple bucks, no need to waste time driving to a store. But they should fit for the camera model that's used - if the camera lens even has a threaded ring to put a filter or something else on there...
As always, a great video Alex ;)
This was great! Am keen to see those heart muscle cells tugging away while jelly wrestling. More episodes with Sasha please.
I love this concept, hope to see more great Lab Next Door videos in the future. By the way, that shirt looks really nice on you. Your videos always make my day a little better, thanks!
I like this series. As an aspiring geneticist myself its interesting to see what goes on in labs.
Oh I wish there were videos like that not only about bio-technology, but also aboout things like how someone is developing a better polymer for an organic light emitting diode (or an OLED display), or how someone is developing a new and better chemnistry for rechargeable batteries or things like that. This would be much more "up my alley" than all that bio-stuff, LOL ;)
Those are probably more locked away behind secret doors to allow companies and researchers to patent their discoveries and profit from them (or at least the University might). If they showed stuff while still researching, someone could beat them to the punch at the USPTO or elsewhere (like Europe or China).
Daemonstorm Animations You might be right about that. Although, if it's still some kind of basic research, it couldn't be patented, because there is no actual product as a result. Also, what I mentioned were just examples...
I really enjoyed this video. Keep 'em coming. =)
Will do! Hopefully these positive responses will help me convince more grad students to let me film and share their research too!
i would have loved to see some actual data. I have never seen this kind of assay, but it seems like a really cool and sophisticated technique. Only with what you showed us I can't imagine how to evaluate and draw conclusions from these kind of experiments
1) Why do they call it aspiration? Because that is the specific technique she's using to take the liquid off it. There are many ways to get rid of the liquid. She could pour it off, paper towel it off, shake it off... but each of those methods have their own end results for the thing in question. So, she was told to aspirate it off.
2) proteins aren't coated in hydrophilic coatings. They are composed of various amino acids and fold based on what those amino acids are willing to be around to reduce their internal energy and therefore the overall stress on the protein strand. So, when in water (which proteins tend to be as organic beings are bags of mostly water, heheh) they fold such that the hydrophobic regions cluster near each other to exclude water from being near them while the hydrophilic areas fold to face out towards the water.
3) polymerization isn't about turning something from a liquid into a solid. It is about growing things from monomeric bases to either long strands or networks of strands. Proteins are polymers composed of amino acids that are covalently bonded to each other. Hydrogels are able to absorb water into their internal structure as the various strands connect to each other (usually be simple hydrogen bonding and not by covalent or ionic bonds) creating a network of twisted up fibers that trap the water inside. This is why it is soft and squishy and deforms under pressure as it isn't an actual solid.
This is really cool! I love your videos
That was awesome :) Thanks
You might want to experiment with a polarizing filter for the camera to cut down on the glare and/or increase the color saturation which can make for a prettier picture. But you'd probably mostly use it for cutting out glare.
Nice Star Wars reference there "The Force that's produced by the cell", LOL.
Sorry, I can't help being weird today ;)
Oh man. I need to ask the guy who named all the microscopes if that's why that one became Yoda. The other one in the room is Chewbacca...
Alex Dainis Awesome! We nerds are the same everywhere... ;)
Oh, I thought the Yoda picture was a poster of inspiration. I believe he is delivering the "Do or do not there is no try" line during that frame, (well not during the frame but you get what I mean.)
How are the rubber stamps made? (eg: Photolithography? Laser etching?)
"Do or do not there is no try"
:)
Would it be possible to get the results of Sasha's experiment?
Thanks, I am looking forward to more videos from this series. :)
Yes, when they publish their papers.
Daemonstorm Animations What's the address?
:)
So I may have missed it, but what is the question that Sasha's research is trying to answer?
They are studying the forces applied by cells within a gel. ie: if you put a heart muscle cell here (or there), how powerful is it, how far away can it push things, etc.
Located midichlorians, you have?
Funny to hear someone with the exact same accent as you :)
Why I feel like you're contaminating the whole thing by putting it on the table? Are those tables clean?
that was the unused left-over gel.