Have we reached the resolution limit of microscopes?
Вставка
- Опубліковано 25 лип 2023
- The resolution limit of microscopes is defined by Abbe's equation and has been reached already a long time ago. There are, however, other developments in microscopy.
🎈 SUPPORT - Become a Patron: / microbehunter
👜 AMAZON AFFILIATE SHOP - As an Amazon Associate I earn from qualifying purchases.
US/World: www.amazon.com/shop/microbehu...
Canada: www.amazon.ca/shop/microbehunter
Germany/Europe: www.amazon.de/shop/microbehunter
UK/Europe: www.amazon.co.uk/shop/microbe...
🖂 NEWSLETTER - www.microbehunter.com/newslett...
💻 WEBSITE - www.microbehunter.com
RECOMMENDED MICROSCOPY PRODUCTS (Affiliate links)
Stellar 1-T (Achromat): amzn.to/3lD7QkA
Stellar 1 Pro-T (Plan): amzn.to/3Adikeu
SW380T: amzn.to/3fB76c4
SW350T: amzn.to/3AjxE9G
SW150 (introductory microscope): amzn.to/3jzCbOx
Stereo: amzn.to/2Vw0Jzs
Slides, Cover Glasses: amzn.to/3jvIJxt
Pipette: amzn.to/3xA8QZi
#microbehunter #biology #microscope - Наука та технологія
Not for amateurs but two months ago researchers published a paper in Nature claiming they had reached Ångström resolution using a commercial microscope. Rather than improvements in the optics, most improvements these days are in the algorithms that process the images and artificially enhance them.
Just Exactly right. Digital cameras producing digital picture data which only then can then be digitally "processed" in unlimited ways to produce virtually anything, anything!
I think in some areas there's room for improvement, maybe less with the theoretical limits but with practical limits, meaning reducing aberrations, improving field flatness, increasing the even distribution of sharpness. For very high magnifications there may not be a lot we can do but for 5-60x there's still a lot that could be done.
I can see the same things through 50 and 100 year old objectives that I can with my new Nikon CFI60 objectives. The modern ones have slightly better contrast from modern coatings and have wider, plan fields. I have 50 year old, 1970s Zeiss plan apo objectives that are amazing to use, especially a 63x, na 1.4 oil objective.
Excellent summary. Just to add - advances in open source self-built microscope systems - like the 3D printed PUMA - put advanced microscopy techniques into the hands of ordinary people (not just well-funded research professors) - but then I would say that! Also, another advantage of LED illumination (done properly) is that is can give a good approximation to a point source unlike the halogen elongated filament. This is a subtle point (no pun intended) but can make a difference at the extreme limits of numerical aperture optical imaging so is relevant to the question.
Interesting, how does the cost and measured resolution compare to say a $400 AmScope T490B? I opted for the halogen lamp because none of these microscopes provide CRI specs for the LED so I assumed it'd be dog water
Yes the PUMA is an amazing device. Lots of work went into it. I started building one, printing one piece at a time and to me , it is the best way to learn how a microscope works including the most advanced lighting methods.
@NavinF Dog Water...lol...is that an Industry term?
The lens coatings get better improving contrast.
One of the biggest disappointments in microscopes is the lack of depth-of-field for higher magnification. If we could use piezoelectric focus scanning and real-time image processing, maybe we could get a larger depth of field on camera/computer views?
There are ways to add game changing improvements.
Page 129: theses.gla.ac.uk/82531/3/2021aidukasphd.pdf#129
@@ThisMicrophoneSoundsCheapit's been a long long time it's been a long, long time since I've had to deal with math on that level (only in school). The text and diagrams are immensely informative and a pleasure to go through.
The person that put that together is well-deserving of a PhD.
Thank you *very much* for posting that link.
I saw this on a silicon chip inspection microscope. But it was very expensive...
Thank you from indonesia🔥✋🏻
I worked on making a cheap fluorescent microscope in grad school, it couldn't get the same quality of image as an industry standard piece of equipment, but it was definitely functional. With 3D printing and optics getting cheaper I'm excited to see what hobbyists will get access to in the future. Maybe one day we can have a casual atomic force microscope in our home offices 😅🤩
There is already a DIY AFM you can build with bits and bobs - not tried it myself as AFM is not my thing. However my own PUMA microscopy project brings advanced high quality microscopy to anyone with an Ender-3-grade 3D printer - including fluorescence, epi and phase.
@@PUMAMicroscope Yes, I checked out your page - very exciting! I hadn't heard of the DIY AFM, will definitely check it out.
Hi,
about the physical limit of resolution, it depend on the wavelength of the light so you can increase the resolution using UV light.
Also, recently I came across an article: "Quantum entanglement of photons doubles microscope resolution".
So it seems optical microscopy can still be improved. However I don't know how expensive it is supposed to be.
About infinity corrected optics, I think image is really improved in light reflected microscopy because you will not have ghost image caused by the half mirror.
And for phase contrast microscopy, I'm pretty excited to see projects like the open source microscope PUMA, because he is working on that, among other cool things.
A nanosphere microscope can gather light more efficiently approaching but obviously not close to a SEM.
Good morning: I'm sorry to ask this question here, as it has nothing to do with the video, but I had to ask it in a recent video to get an answer. I recently purchased a simultaneous focus trinocular zoom stereo microscope for use in electronics, but I have never used any microscopes of this type or any other before. When using it I came across a situation that I don't understand why it happens. Here's the thing, whenever I try to view an object through the eyepieces I can only see clearly if my eyes are a little bit away from the eyepieces and not touching them completely. When I place my eyes completely against the eyepieces, I simply fail to see the object and everything is dark. Could you clarify if this could be a microscope defect or why this happens. Is there a technical explanation for this? I find it more comfortable to work if the eyes could completely touch the eyepieces but is that not possible? Congratulations, your channel is excellent.
Greetings, thank you.
I just picked up my first hobbyists scope, an olympus CH30! I'm enjoying it so far but it is very barebones and I'm struggling to find used parts online. I know you have the bigger brother, the CH40 but it looks like you have some older BH2 accessories attached. Parts for these scopes appear to be alot more abundant and I'm just wondering if they are compatible with the slightly newer scopes
If you use a uv camera or a camera that images a shorter wavelength, would the reselution be increased?
Yes
Question: I’m wondering if I were to “double up” on the lenses, for example use two stacked rather than one lens on every lens on the microscope.. can this give me a stronger, more detailed outcome?
Hi. I expect some noticable adventages using KI image technology. For example there are papers describing how to increase the depth of field using deep-learning networks. I could also think of soöutions switching quickly between different imaging modes for example between bright and dark field and let the computer combine the two images to get a new more detailed one.
A third idea would be to change the focus distance mechanically as a high frequenzy and record multiple images with different depth of field and combine them to one image. Smartphones already di that for example switching between the different cameras.
It would also allow for the creation of 3D images, possibly improving the experience even further.
Also cromatic aboration could be removed using deep learning.
These are just the things i came up with on my own there are probably many more ideas using differnt wave lengths ans sensors who can see these wavelengtjs and using computers to translate these images to human viewable images in real time.
I expect a lot of change in this area and expensive new microscopes as a result.
Can you review the amscope b120b???
Thank you for your content! I have a question and hopefully you can give your opinion. I am a high school Biology teacher. I want to buy a budget friendly microscope with a digital display, but I am not sure what brand is good. Any info you be greatly appreciated. Thank you!
deconvolution microscopy...
I’m looking for a nice microscope 200-300$ heavy compound microscope, is there any recommendations?
Depends on your definition of nice. You can get a cheaply made Chinese one, with poor mechanical construction, no dealer support or spare parts, that will give you pleasant views for hobby use.
Or, you could search for a used professional one that originally costs thousands of dollars 40 to 50 years ago that will give you the same or better views and have vastly superior mechanical and material quality. If you are in the USA, America Optical has the best value for the money for used microscopes.
i buyed me 2 use Wollastones and for 100 bugs i had DIC and forgot PH
light-microscopers are blind..........look at the resolution of a REM...electron beams are also electromagnetic waves
There is no limit of resolution, there is only a limit of details you want to see. People who search for high magnification often will find things which they dont want to see.......
Details = resolution
@@joachimb5721 uo only see what you want to see and what they teached ou even with the best resolution some are blind
First :)
you cannot compare scientst with aestheticists, and please dont shout them hobby-mikroscopers
there is no hobby microscopy, if you think that you are a hobby-microscoper you should be more diligent