Determination of Iodine Value _A Complete Procedure (AOAC 920.159)
Вставка
- Опубліковано 10 лип 2024
- The iodine value is the mass of iodine in grams that is consumed by 100 grams of a chemical substance. Iodine numbers are often used to determine the amount of unsaturation in fats, oils
This video represents a complete procedure for determination of Iodine Value of oil samples.
________________________________________________________________________________________
Follow us on Social Networks to Stay Connected:
UA-cam: / @microchemsexperiments
Facebook Page: MicroChems-E...
Be with us, be a Scientist.
________________________________________________________________________________________
Related Tag:
#iodine
#iodinevalue
#Oil
#Oiltest
It's amazing that this channel makes it possible to practice straight away, making it very easy for us to work in the laboratory. Thank you🙏
Thank you
I can never find someone explains better than you did . Thank you
Hassiba Hassani, Awww, How sweet. Thank you so much for your comment. Be with us. ^_^
I really appreciate your effort for showing as such an amazing virtual lab. All the description was exactly in touch with each and every moments of the whole experimental framework.
# as a reminder, please present as how wijs reagent is prepared in lab. Thanks.
Okay. Thank you so much.
@@MicroChemsExperiments Como saber se o carvão ativado está acima de 800% de iodo
Great presentation! Please continue the good work. Knowledge is for sharing.
Thank you so much. Stay with us.
I have learn a lot through this your channel sir
Thanks for bringing value to this noble channel.
I appreciate your effort.
Glad to hear that. Thank you
This is the best channel I have ever seen, thanks for your effort ❤
Thank you so much. Where are you from Ma'am, may I know?
@@MicroChemsExperiments Egypt
@@esraamossad3970 You can contact us in Facebook also. Link is given in the description section.
@@MicroChemsExperiments I have already followed you on Facebook, thanks MicrChem ✌
@@esraamossad3970 Thank you Ma'am. Stay with us.
Thanks for your wonderful and passionate video. It's really useful for my work. I wonder some point that could you explain for me, please.
1. Why we certain use Carbon Tetrachloride in this experiment. Can you explain in detail the reason why you use Carbon Tetrechloride? Can I replace it by other substane?
2. I wonder if you put Potassium Iodide crystal around the stopped? Why don't we use the solution of Potassium Iodide?
3. In the phase that you pour distlled water into the flask, you titrate the flask with Sodium Thiosulfate until the yellow is lighter before you add the starch solution in the flask. So I wonder the reason why you must to titration before adding the starch solution. Is there any theory in this phase? I really want to know.
I truly appriciate your support and I am looking forward to hearing from you. Many thanks
You are awesome! Thank you for sharing!
Thank you for supporting us.
it's good job.thank you very much
Before do any lab experiment i search it your channell.it is very usefull to me. Though i get wuections😉😊😊😊😊
Kasthuri Kk,
It's our pleasure ^_^
We are planning to make videos for almost every laboratory tests. Just be with us.
Thanks for sharing with us your valuables information
You are most welcome
What an excellent explanation ❤❤ thank you so much
Thank you
I like your explanation and demonstration
Thanks
Thank you too. Stay with us.
All tests which i need in solvent plant laboratory testing, all tests are there on your channel thank you so much
Hi, thanks for making this video! It is really helpful. I have been doing my experiment about how the number of times heating the corn oil till boiling will affect the oil’s iodine value and I realized that as I did titration, there was a layer of oil-like solution at the bottom of the flask. Do you know anything about this and why does it happen? Because it has became really obvious and disturbing since the oil darkened as the heating number increased.
Hello, may i ask what were the molarity of the Wijs solution and carbon tetrachloride used here? I would like to see how i can change the calculations if i am using different concentrations
Wijs (iodine monochloride) solution: 0.1 mol/L
Carbon Tetrachloride: 99%
Thank you so much for your important video❤❤❤
It's my pleasure
Appreciate your effort on preparing such useful videos.
Thank you very much. Stay with us.
1. Quality testing of raw as well as finished products of confectionary.
2. Preparation of: 1. candies, 2. Caramel, 3. toffees.
3. Collection of various types of confectionary packages.
4. Determination of sugar in confectionary product by saccharometer.
5. Determination of refractive index of sugar - solutions of different consistencies
Pls make vedios on this tests
Okay.
Thank you so much
@@AbhishekSharma-mo3nb you are most welcome
You are just superb!!
Can you plz make a video on sugar determination from carbohydrates...i mean fehling solns..at which point we can consider the end of the titration.
Astha Jain, Thank you. We have already started making video for Sugar determination. Stay with us.
Informative video tutorial.
Thanks
Thanks. Was helpful for exams in my masters Thanks a lot
Thanks
Hi MicroChem´s, can i make this determination of iodine value in activated carbon??
thank you
You are welcome
Ihave iodine mono chloride in the labe but if iuse it it doesnot give me the good reading for the blank low readeing what can ido any way to increse the wigis solution strength
Nice your analysis
Saroj Sahu, thank you for your comment. Stay with us.
this was so well explained💝
Thank you
Its It's very good to see experiment very clearly I have thought our chemists by showing the video... many thanks...
Please update USM UNSAPONIFICATION MATER ALSO
NUTHAKKI RAJESH, okay we will work on unsaponification mater as early as possible. Be with us.
@@MicroChemsExperiments thanks
NUTHAKKI RAJESH,
This video will help you. Please take a look.
ua-cam.com/video/DpgmHx-dl1A/v-deo.html
Hi love the video！Just wanted to ask why put the flasks in dark for 30 min？will the time of this have an effect on the titration?
This is done to allow reactions of some light sensitive chemicals.
hi , I enjoyed the video but shouldn't the sample amount been pipette out instead of poured in because the majority of the sample was on the side of the flask not at the bottom
Thanks for your knowledge sharing... please make a video on "urease activity of soya bean meal"...
Ok...we will do it soon. Stay with us.
Very informative video
Glad you think so!
Thank you for this vid. I just want to know, how do you handle these chemicals as waste after the analysis?
We have third party company to handle this
If the blue color keeps coming back within the 30s, what could be the problem?
Right after turning milky-white, the blue color returns. This happens repeatedly even after titrating more of the thiosulfate
Thanks
Welcome
My question is not about AOAC but about AOCS methods, that before weighting the sample, sample must adjust temperature to 68 -71 celsius deg. I wonder what is the purpose of sample temperature adjusting?
Chemistry is all about chemical reactions. Chemical reactions takes place differently at different temperatures. So the adjustment of temperature is very crucial for any chemical analysis of sample.
Can please ask why are we still multiplying with normality if we have converted to mass already?
Also when I carry out the procedure, there is a purple coloured layer at the bottom of the solution in the blank and a pink coloured in the sample, do you know anything about this?
Nothing noticed like that. Prepare chemicals and reagents freshly and try it again
Thank u so much ...☺️
You are most welcome.
thank you for making a test video tutorial like this. the video is very clear and easy to understand. It's just that there are some points that explain it too quickly, so I feel that there is something missing, but it turns out that the explanation is just too fast.
@Nadia Qonita,
You are most wecome and thanks to you for your observations. We will try to make our videos more clear.
Which oil u have used in this pls tell
Can you guide me how to calculate ! Especially the blank thinks
how does heat affect this experiment? how would I heat the solution after keeping it in the dark if I want to check temperature affects on iodine value?
You can set an experiment by applying heat and let us know the result. But I thing heat will affect the test result because iodine will be removed from the flask during heating and a false result will come out from your experiment.
This was great work. One question, wouldn't the KI crystals placed on the top interference with the reaction?
Aksania Morris, thank you for your comment. No, Addition of KI wouldn't interfere the reaction. In AOAC it ia said to add KI.
@@MicroChemsExperiments ok thank you
@@aksaniamorris3042 Welcome. Thanks for being with us.
Actually the KI helps to liberate the unreacted I from Wij's solution
@@MicroChemsExperiments
You can add 10% KI solution 15 ml into flask,
How to prepare wij's solution??
50ml iodine monocloride desolved in how many acitic acid in ml??
Please🙏 reply
nice
Shrikrushna Chaudhari, Thank you very much. Be with us.
Hi. Why did you not add 10-15% KI b4 stopping with water?
Thank you!
You can add 10% KI solution 15 ml into flask,
please how can Ido iodine value in oil by automatic titration machine ihave thermo brand t940 orion this test is redox and back titration but cant to do the steps can any one guide me
Who the hell disliked. Idiots...perfect demonstration. Pls low noice back ground sound adjust. We lv your channel.thanks. plc to hplc aflatoxin analysis
@Kasthuri Kk,
Ha ha ha...1 dislike among 89 like is negligible. They have rights to express their opinion. So plz don't argue with them who disliked. What you can do, you can love the channel more & more.
We will make the background sound lower from the next video.
Which aflatoxin (among B1, B2, M1,M2,G1or G2) do you want video for?
B1,B2,G1,G2 now im currently initiatimg in my lab sri lanka.if you can pls do other also.it will be really usefull.u guys are amizing.
@@Titi-je3ff Okay. We will make it as early as possible. Thank you for your comment.
AOAC Official Method 920.159
*lodine Absorption Number* of Oils and Fats, this is the method that I referred to.
Yes
I watch iodine value analysis video many time.which is very good & clear.but i want to know without putting iv flash in dark test can be done?
Putting iv flash in dark is necessary
Thank you for sharing. may ask question why add KI at neck of flask when during preparation of sample and blank?
It is said to add in AOAC.
@@MicroChemsExperiments 👍👍
Bcoz iodine vapours doesn't go outside of iodine flask
You can add 10% KI solution 15 ml into flask,
Where can I buy or how can I get the AOAC manual for the different methods? I'll still keep watching you! 🙏🐾
You can purchase it from Amazon.
Full meaning AOAC
Nice good explanation,i have one question,Why we kept sample in a dark place for exact 30 minutes.
To allow the chemical reaction satisfactorily that could be halted in sunlight
Hi! where is the sample based on the expecetd iodine value table from? I cant find it on the internet
The expected value of different samples is given nowhere at a place. You have to find one by one. Say for example, you want to test iodine value of soybean oil. Then search on google by "Iodine value of soybean oil".
***Google will show you normal iodine value of soybean oil.
***Another source is Published Journals about "Studies on Soybean Oil".
***You can also find the normal iodine value range of your country's "Standard Of Soybean oil"
Search the value following above 3 ways and get the idea.
NB: If not works above steps, then test your sample thrice considering medium, high & low value. Then you will get an idea about the expected Iodine value of your sample.
Thank you.
Constanza Uribe Cejudo
Heyyy , i can help you in your exams and assignments , i am a medical expert .
I have been given unknown kind of fat or wax maybe but the point is this sample is in solid nature, how will I predict it's Iodine value so that I'm able to weigh the supposed weight of the sample?
Go for the first trial taking any amount of sample which will give you an idea about the iodine value (approximate).
After getting the idea, go for the second and final trial taking sample weight according to the iodine value you got from the first trial
Ma'am I have a simple question what is purpose of this test or what does tell us this test?
Hi could you please let me know any alternative for CCL 4 ( apart from chloroform ). Thanks
No replacement to be known for CCl4 other than Chloroform
@@MicroChemsExperiments thanks alot for the reply
Hi, thank you for the video! But I have a question. What is the purpose of adding potassium iodide crystal? And can you quantify the amount of potassium iodide crystal added around the surface of the stopper? I'm a student planning to do this experiment in schoolwork.
Potassium Iodide helps to release Iodine. You can add 500mg into the flask
@@MicroChemsExperiments Thank you!
Very useful information, however one issue tho, you do not heat a Volumetric Flask!
Noted
Hi, what's the concentration of wij's solution did you use?
1.623%
what does the potassium iodide do, is it needed for the experiment
Yes. It is needed
It was great work. I have a question?
Will there be any problem if I use saturated KI solutions?
You can use saturated KI solution
@@MicroChemsExperiments ♥️♥️♥️
@@MicroChemsExperiments
Can you give me your number, please?
@@user-jk6yb4wz3s knock in our Facebook page. Link is given in the description section
Hi, I was wondering why is it so important that it should be closed immediately after pouring the wij's solution. By the way, this is a great video it has helped me so much. If you have any other papers, videos, or references about determining iodine value in oils by wij's method I would be thrilled If you could reference it. Again thank you.
Ekin Gursoy, Thanks for your comment. Actually, when Wij's solution is added, it reacts with Iodine present in the sample to form slightly volatile compound. If we left open then loss of iodine may occur. That's why we keep the flask closed immediately after adding carbon tetrachloride and Wij's solution. Other two references for this test are: IS-548 and FASSAI MAnual.
@@MicroChemsExperiments thank you very much
@@ekingursoy1779 You are most welcome.
@@MicroChemsExperiments Great video, very interesting to watch. However, why was the iodine crystals added around the stopper after closing?
@@khemist2631 It is added to set a barrier for passage of gas from the flask to the outside during the 30min of the reaction time.
Why don't we add starch indicator in the beginning of titration? What only when the colour of the solution becomes lighter?
It is a two spet titration. More accuracy is achieved by adding starch when the colour is faded out.
hello! what is the solvent used fir wij's solution?
can water be used?
use acetic acid as solvent
Can you give me the "Determination of Oil Analysis" manual if I need it?
We do not provide any document
What is the unit.? Of IV in this calculation.?
what is the difference between this method and ASTM D4607-94 method? Can I use this method for activated carbon?
No. Our method is for foods and oils.
We will prepare method for activated carbon later. Stay with us.
@@MicroChemsExperiments oh thank you very much, looking forward to it.
@@user-df5is8jy6n Welcome.
Can you please help me with a soft copy of the method
Thanks
i took 0.216 gm sample of groundnut oil its value came 93. isit in expected iv range or this answer is wrong
Hi Mam Is it possible to analyse cholesterol content in edible oil by volumetric titrations..??? Without chromatography or spectroscopy plzz reply me mam...
We will upload video later.
Super
Thank you
plz also give the reason behind adding any chemicals in the given experiment .
please make a video on henus method.
Noted. Ok we will make it. Stay with us.
Where Didi you get this 12.69
Please post a Video for Determination of TVBN in Dried Fish Meal sample AOAC
Noted
There's a mistake in your analysis the equivalent weight of sodium thiosulfate is 248.16g so if we want to make 0.1 N Sodium thiosulfate we need 24.6g not 2.48 g
Please how to prepare Ipa lactic acid solution for sv test of wheat flour.
Did you mean sedimentation test of wheat flour?
Thanks for the video,
But if result 38 for olive oil and compared with reference rang and show less than from rang what writing comment?
The quality of that oil is not good and maybe mixed with other cheaper oil.
For the blank , we should to find iodine value also ?
Not iodine value actually. Blank reading represents the chemical error for iodine value test
Sir please make a video for urea activity check for foods all 3 method
Ok
i have prepare Wij stock solution using 50g ample in 500ml (makeup)acetic acid and for working solution i have take 80ml of stock solution and makeup with 500ml of acetic acid is this correct
We will make video for the presentation of wijs solution soon
Hii! first of all, your videos are very helpful. But may i ask something, why is blank titration necessary in the this determination(iodine value det.)? I hope you answer me ,thank you so so much
Blank titration is needed to subtract reagent reading.
@@MicroChemsExperiments Thank youu Ma'am/Sir.
@@biancaysabelvicente215 You are most welcome.
Heyyy , i can help you in your exams and assignments . I am a medical expert.
Hello, what oil is used in the sample oil?
Coconut Oil
Iodine molecular weight is 126,90477 g/mol . Why was this (12,69 g/mol ) written in the formula?
Plz share video on determine residual chlorine in water
Maham Rafi, Thank you for your comment. We will start working for the chlorine residue test of water very soon. Be with us.
Any reference book for this experiments
Rakshinda Sayed, Yes. This test is strictly followed by the method 'AOAC 920.159'.
What is filter paper number used to prepare 1 percent starch solution?
Number 1
@@MicroChemsExperiments can we use whatman no. 4 filter paper?
After preparing sample in IV flask And during kept in dark for 30 minutes why we use KI Salt on the top of the cap of the flask ??
Goutam Gupta, Thanka for your comment.
According to AOAC method, you need to add KI. KI crystal is added on the top of the cap. The reason is: it prevents the transportation of volatile substance from the flask to the outside environment. Rather KI crystal absorb the volatile material from the cap and enter into the flask during washing with water.
@@MicroChemsExperiments thank for clearing doubt .
@@goutamgupta8816 you are most welcome. Stay with us.
Pls one video iodine value in activated corbon
Noted.
1. During titration, iodine flask should've been covered with the stopper throughout in order to avoid the escape of iodine gas.
2. calculation should've shown the units also
3. It should've been 126.9 g/mol, since that is the molecular weight of iodine.
Regarding 3.: Yes, but you have to divide by 10 to get the correct unit for the IV, which is g(I2)/100g(fat). It would have been obvious, if the correct units were used in the video.
Noted
Mam jo dark m rkhte ho vo only 30minutes which type of dark you use plzz tell
I used cabinet where no light can enter.
Blank me starting me kya liya tha befor adding ccl4
I need a blechability test for cruid oil ,plese share
Noted.
What is the 12.96 in the formula.Can you tell me.
Calculation factor
Why uou mention 12.69 Can You explain plz
Can you please tell me the chemical equation for this reaction.
We will make video later for every test explaining chemical equation
Why initial value sample of 25.6 ml and blank 2.7 ml take in burette
We started titration of sample first. In this case, initial reading was 25.6 and the titration was finished when the final burette reading was 39.2.
We refill the burette again and start blank titration when the initial burette reading was 2.7 and finished when the final burette reading was 39.1.
NB: we did not refill the burette completely thats why the initial burette reading was started at 25.6 and 2.7 for sample and blank. There is no specific reason for it. You can start both titrations (sample & blank) from 0, 1 or any other initial point. There is no limitation in it. It's up to you that from which point you want to start.
Plz add the vedio that minaral oil test in edibal oil
Noted