MALDI-TOF Mass Spectrometry to Identify Gram Negative Bacteria | Protocol Preview

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Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry - a 2 minute Preview of the Experimental Protocol
Timothy J. Gray, Lee Thomas, Tom Olma, David H. Mitchell, Jon R. Iredell, Sharon C. A. Chen
Westmead Hospital, Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research; Westmead Hospital, Centre for Research Excellence in Critical Infection, Westmead Millennium Institute; Westmead Hospital, Sydney Emerging Infectious Diseases Institute, University of Sydney;
The application of matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry (MS) directly to blood culture broth expedites the identification of bacteria. The presented method is a rapid and reliable method for identification of Gram negative bacteria directly from blood culture broth.
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