NCBI Minute: How to Submit Your 16S rRNA Data to NCBI

[@marwatawfik3956]

choosig illumina what should be the assembly program?

[@nohataha6398]

what if when using blast for unknown it gives 75 similarity in identity??
any help

[@DrDeepakRawal]

when i am submitting cox1 gene sequence it is showing error of cds frameshit how to fix it?

[@a3tube471]

same to me

[@bryanjose3229]

I have my eukaryotic RNAr 26s. How can I submit it? Thanks

[@FaBi-ws8mv]

How can I submit my eukaryotic RNAr 26s?

[@abrhamulu9610]

Is it applicable 16S rRNA sequences from metagenomic DNA in Fastq format? how do I submit sequences from metagenomic DNA of more than one samples under a single submission?

[@aumshreesorathia9877]

where can I find webinar material ?

[@nicolebotha9284]

Who disliked this? It was really helpful

[@DrJenny-pv3xk]

Plz tell me the accession number

[@showme2youtubication]

Thank you for the tutorial. My sequence is UNVERIFIED. so what should I do? how to overcome that. Kindly help.

[@benjamingillard9603]

Thank you very much! This helped me perfectly to submit my sequences.

[@thomassmith381]

If you have more questions about submitting sequences to GenBank, please contact gb-admin (at) ncbi.nlm.nih.gov.

[@asmaemisbah2230]

plz can i submit sequences with only 500 pb?

[@samdz6202]

Yes, you can

[@saptootpas]

I have ITS sequences which are located between 16S and 12s RNA. That possible submit by following your guideline? Thanks

[@thomassmith381]

Yes, ITS sequences can be submitted through submit.ncbi.nlm.nih.gov/subs/genbank/ .

[@antisadboilaser]

For the submission of reads done on something other than Sanger such as Illumina i'm a bit confused as to why they require assembly. Do you normally assemble 16s rRNA sequences? Or does assembly mean something different in this case?

[@thomassmith381]

Individual reads should be submitted to the Sequence Read Archive, which is a separate database at NCBI. The assembled versions are accepted into GenBank.

[@geetarani2875]

thank you very much mam/ sir its very informative

[@merakilousrhythmz3997]

Thank you so much! This video helped me a lot. 😊😊

[@poulamidatta8823]

Thanks a lot.. Very helpful for the sequence submission..

[@khanaziz7875]

very useful webinar, thank you

[@terrysharpe4808]

Khan Aziz

[@wanmuliati9095]

Thannkyou very much, this tutorial help me a lot.

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[@DrRoma-nq7sl]

Really helpful

[@biotechnologymahasarakhamu4757]

Thank you for this. Helps a lot.

[@farzanehadel523]

great! the video is really helpful

[@suleimanallison7680]

Thanks very much , higly helpful and well understood.

[@amarabhishek614]

thanx sister....

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[@obinclk2890]

this was helpful. thank you.

[@x-b5516]

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[@brisesonne]

Thank you so much, this video is very helpful...