My first thought when I saw the image of the last plant was that it was Dracaena Sanderiana aka "Lucky Bamboo". So I think your matK result came close, but may not have determined the exact species.
Use a CTAB DNA extraction, then use a modern(!) ITS2 primer and you will get to species level much more reliably. Don't forget DMSO in your PCR if using ITS2. Check the date on the rbcL+matK paper, it's outdated.
Good day, am Kceee. Like your honesty in your report. Am working on something similar. I used rbcL and ITS to barcode 7 plants. I want to compare the results to establish which is a better barcode. Could you give me a guide on some analysis I could do.
Thanks for the feedback 😊 Have you got results from both rbcL and ITS for all 7 plants, and are the sequencing results different? We have a guide on our website that covers the protocols Jenny uses in this video, and works for both rbcL and ITS barcoding bento.bio/protocol/dna-barcoding/
@@BentoLab Yes I have. Please could you advise me on how to analyze the interspecific and intraspecific divergence and also check for barcoding gap. From different literatures I have gone through, these methods were used to determine which barcode marker is best for a particular plant family. Thanks ahead.😊
@@kelechiejindu5487 That goes beyond our scope of bioinformatics knowledge on our team at the moment. Sorry we can't help more - good luck with the project!
Hi, you can find us at bento.bio, and you can check out our DNA barcoding pages on our website here: bento.bio/protocol/dna-barcoding/ . If you have any questions, send us a message via the Contact Us page with details of what you'd like to learn and I'll see if I can point you towards some useful resources.
My first thought when I saw the image of the last plant was that it was Dracaena Sanderiana aka "Lucky Bamboo". So I think your matK result came close, but may not have determined the exact species.
Thank you for explaining this procedure so concisely even a newbie like me can understand. But, how to choose which primer to use? Thank you.
Use a CTAB DNA extraction, then use a modern(!) ITS2 primer and you will get to species level much more reliably. Don't forget DMSO in your PCR if using ITS2.
Check the date on the rbcL+matK paper, it's outdated.
Thanks Adrian, that's awesome feedback!! What species do you barcode?
Bethan
Good day, am Kceee. Like your honesty in your report. Am working on something similar. I used rbcL and ITS to barcode 7 plants. I want to compare the results to establish which is a better barcode. Could you give me a guide on some analysis I could do.
Thanks for the feedback 😊 Have you got results from both rbcL and ITS for all 7 plants, and are the sequencing results different?
We have a guide on our website that covers the protocols Jenny uses in this video, and works for both rbcL and ITS barcoding bento.bio/protocol/dna-barcoding/
@@BentoLab Yes I have. Please could you advise me on how to analyze the interspecific and intraspecific divergence and also check for barcoding gap. From different literatures I have gone through, these methods were used to determine which barcode marker is best for a particular plant family.
Thanks ahead.😊
@@kelechiejindu5487 That goes beyond our scope of bioinformatics knowledge on our team at the moment. Sorry we can't help more - good luck with the project!
@@kelechiejindu5487 What do you mean by barcoding gap? rbcL has no barcoding gap. You are mixing up COI barcoding with plant barcoding.
When the results are so close together, than its rather useless to find the plant. Unless you have a really small sample of the plant.
hii mam
i am intrusted in learning DNA sequencing
plz can i get your contact detail if its okey with you?
Hi, you can find us at bento.bio, and you can check out our DNA barcoding pages on our website here: bento.bio/protocol/dna-barcoding/ . If you have any questions, send us a message via the Contact Us page with details of what you'd like to learn and I'll see if I can point you towards some useful resources.
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