Agar tek and trick that can speed up growth!
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- Опубліковано 1 лип 2024
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Music by D'Rok The Menace ("ether")
11:33 "lets jump right into this video" i love you willy
thank U
I was looking for this comment. Thank you.
Thanks 😊
Thank you so much.
lmao literally knew to look right at the comments because my man willy always be having the longest intros.
10 g agar
10 g light malt extract
2.5 Tablespoons Activated Carbon
500 ml of Water
Pressure Cook for 30 min at 15 psi
Video starts at 11:38
🍄🍄🍄🍄
Mush love 🙌
THANK YOU!!!!!!
Thank you for that, saved 10 mins from my time
Thank you!
Thats a super soft recipe
10g of agar/ 10 g of Light malt extract, 2.5 TBLSPN of activated carbon and 500ML of very hot water.
Don't forget to pressure cook for 30 minutes at 15 psi.
Activated carbon powder works best, it makes it Black Hole black, like light doesn’t escape it black….
what's the carbon for?
@@onsigon Some people have said it helps with old spores, but I think most people use it to make the agar black and the myclium easier to see for isolating a mono culture during transfers.
@@onsigon to make Greta mad
If Willy didn't teach this stuff like he was teaching a 5 year old, I would still be puzzled about a lot of things
the thing is .. its only really slightly anti bacterial , dows nothing to "increase growth" and is visually great for seeing white things like myc high contrast , but also so dark that it can also hide certain molds etc .. why we need a 26 minute video ill never know :)
Trip team fam. Love it!! Repair antique pinballs and video games for a living with epilepsy none the less (took 10 mins to type) and now learning mycology!! Thank you for keeping my brain excising!! Frontal lobe damage from service. Pushin 50 brings ya that much closer to nature and God or your perception of. Bless you my man for the education and if ya need electronic/electrical advice, Ive got you.
Mushrooms (and some other types of psychedelics) encourage neuroplasticity with new cellular and nerve growth and repair. Are you using these to help? I know they’ve completed animal trials of using DMT to administer to stroke victims with amazing results and they’re moving on to human trials. They’re trying to also isolate which part aids in healing and if they can isolate that without the trip for those that would not do well with that aspect of psychedelics (this could help a wide range of mental health issues as well), especially in elderly.
I’ve had memory loss repaired and memories returning and a number of positive benefits. I have lupus and had CNS damage from it, as well as developing a kind of “failure to thrive” due to being stuck in an extremely abusive marriage for over 20 years (all money was hidden, sabotaged a successful company and then blog to keep me from making money, moving me 1000 miles from friends and family, and no support from outside family or friends due to this, as well as being gas lit and manipulating everyone into believing I was the problem. My kids knew the truth and just laid into my parents about how bad he really was (they adored him). Anyways, my body was just starting to shut down. And shelters require that you work and I couldn’t (I also have heart, lung, and kidney issues from the lupus.) I felt no hope or end in sight or any way out until I discovered I had some money in unclaimed property, so I retrieved that to secure an attorney and then had police removed him from our home after they were called during one of his rage episodes. And had a locksmith immediately change the locks.
I had trouble remembering much of my life, things I’d learned, etc., but since starting a macrodose regimen with these to treat my severe PTSD my memory is returning rapidly, I’m able to learn and absorb new information again, etc. I feel the old me returning. I hope you find the same relief.
@@TheFrugalMombot this is why I love this community. I suffer with crippling anxiety/depression/ocd and ptsd. You guys give me hope. And for that I love you all 😭✌🏼🖤❤️🍄
Bless you sir and thank you for your service. Glad you’re still here with us and I’m able to read your comment. Mush love ❤️ 🍄
Thank you for your service brother! Younger veteran myself (34 now) and I too got my brain jacked up pretty good while in Iraq and a few head injuries since back in the civilian world!
These gifts from the God's...these beautiful little fungi...are truly a God send for veterans and EVERYONE with trauma for that matter! I've never commented publicly using the word God as a matter of fact! This time last year I probably would have shit on the simple mention of such a being! These gifts have brought so much serenity and clarity to my soul and WillyMyco is one of those responsible for my transformation! So thank you to Willy and the entire Trip Team Family!
You should also use a rack in front of the laminar flow box when working with petri dishes to ensure they stay elevated and within the sterile airflow. Trip Team Family!
he knows this dummy
@@triple_gem_shining Whether or not Willy knew is irrelevant, the comment wasn't for him. This is an important aspect of sterile work that he failed to mention in the video. Noting it in the comments will only prevent people like you from wasting their time making dishes that are more likely to be contaminated. Why do you feel the need to be so hostile about it?
@@ryanwilhite Right? He instantly has to throw an insult out at you for making a legitimate comment. I guess there are always those people looking for anything to insult someone else for since everything they do is so perfect :)
@@kereyc5751 Some people just aren't contented unless they have something to complain about.
Thank you for this. I am new and using a SAB. Since there is no sterile air flow and assuming you aren't stacking the bottom plate on top of others, am I correct in thinking a rack is not needed with SAB?
TRIP TEAM FAMILY ALL DAY !!!!! THANK YOU FOR BLESSING US WITH ANOTHER GREAT VIDEO
Oh yessss got a shit ton of active carbon! PS if anyone's using a still air/glove box for the first time, DO NOT use alcohol to clean it if you're going to be flame-sterilizing.
Haha, nah you should definitely torch sterilize under the airbox AS SOON as you clean with alcohol, you silly goose.
Lil flame neva hurt nobody.
So good to see you doin more frequently again bro 🍄🍄 I've been part of the trip team fam for yrs it's such a shame yt killed your old channel there was so many awesome vids on there that did & still would help peeps out ✌💜🍄🌵
On brand new to mycology I started in october I did some work already. Cloning Swabbing And made up my first batch of Agar.. I just took some charcoal capsules that people use for vitamins. I did notice they were a little granulated. So I use the mortar and pestle Is and made them more fine I added two capsules Roughly one Graham to my premix agar powder Came out fantastic. Also added a little bit of beet root. Powder In half of the batch. Just to spice it up..
TRIP TEAM FAMILY!!!!
Had so many questions about this!! Thanks so much bro! Mush love!
Willys' Drinking Game:
- Take a drink every time he says "you guys"
21:47 benefits of adding the activated carbon
Thank you!
Thank You Very MUSH!
🤠
Trip team family! Love the videos man! I am learning a lot right now. I just snagged a laboratory laminar flow hood from an auction for 26 bucks Canadian. Can wait to get it fixed and and flowing!
Yo I know I’m a year late, but does that flow hood still work for you? I’m amazed that you got ahold of one for so incredibly cheap. Guess I should be looking at auctions
Your videos are always so long, but there is a reason for this, the content is always a comprehensive complete and excellent 👌. Thank you 🙏
Just bought some charcoal . Can’t wait to try it this weekend . Love the black agar . Just the aesthetic alone is amazing
Thank you for another great video! What sorta indicators would I be looking for on black agar to show the agar has gone bad. Will the discoloration still be visible?
Trip team family!!! I’m for sure going to do carbon agar for the dud spores I got sitting around. Good looks bruh! 🍄❤️
TripTeam Family!! Been following along with these vids you put out and mixing things here and there with others and having good turnouts ... keeping me afloat during this quarantine without work! Gotta spend arms and legs to make arms and legs
Trip team family!!! Love what you do Willy!!🍄
Thanks for keeping us updated as always Trip Team Family 🙏🙌❤️
Trip Team Family... May the spores be with you! Always...
heads up to anyone about to put metal in their microwave lol
Thanks for the shout out
Non-ferrous metals are fine
@@SpazziJackazzi go ahead and put a ball of aluminium foil in there then bro
@@whatinxxxtarnation4728 i do all the time, ali foil over bowls. Its fine.
Trip Team family! This video answered all my questions about carbon agar. Thanks!
Trip team family!!!!!! Awesome vids keep it up!!!!!!
Trip team family!!!! Love it!!!
Love this channel, go Trip Team Family!
Trip Team Family, Ive been getting into the hobby and man you're one of the best content makers for this right now!
chronicles of bod
Soooooo awesome!!! Keep it up, Willy!!!
Trip Teen family member! Willy, can you go over sectoring and isolation. I think I've got it figured out, but I love the way you explain things. Namaste.
Trip Team Family. Love you're doing Willy
Trip team family!! Mad love from Philly my man!!!
Trip Team Family For Life!! What an epic giveaway!! Trip hard people 😎👌
Been with you since before the original YT exodus to Vimeo Glad to see you back on the platform man!
Thanks so much for explaining this.
Trip team family
Awesome give away you're doing man, and thank you for spreading all the information
It would be interesting to mess around with different ingredients in the agar in order to see if there is a benefit from each and maybe worth also adding to the substrate. Do you ever use liquid culture? I’m considering that for faster growth. Mossy Creek mentioned it in a video.
Thank you for what you do. Q...AT WHAT temperature is the best to grow agar?
Thanks for all the great information. I'm working on my first grow now and I learned a lot from your videos. I have several jars going for PF Tek and three bags of grains for some mono tubs I'm going to try. Keep doing what you do and mush love to the trip team family.
So how did it go? I’m on my first go now as well! I have MEA agar 🧫’s,LC’s (Karo for one jar and raw cane sugar for the other) and I have some pf tek brf jars going too. Wish me luck!
@@dressedanddepressed2301 howd it go??:D
Friggin love you, Willy
Quick tip: fine coffee grinder is perfect for making sure you get ya charcoal as fine as possible and easy to clean up. Same kinds blenders people use to break down bricks in order to add cut and then use a custom mould with a hydraulic press to 're-rock' it ✌️
Trip Team Family!
That plate would look sick on the wall at my local cannabis club (still illegal here in New Zealand so those guys are putting up the good fight!)
Love the videos brother! Keep them coming
just finished my flow hood. your video was so helpful to thank you. ~~Support The Trip Team Family Movement~~
Im really really ready to start and the best eay is just to do it right. Quit talking about and be about it. Thank you for all your great knowledge
Haha, I love the amount and depth of knowledge you spill, but I gotta get nit picky and complain about the length of the videos lol. Reminds me of myself, once you get me going on a subject I love you can't shut me up! Much love fam! Got my first flush coming in, spent under $100 to start from scratch.
If you are using glass petri or jars can you pressure cook inside the dish or do you not recommend it?
I'm just grateful you took the time to make this video, thanks brotha! I'd like to suggest adjusted the volume of speaking to level with the music, if you can please.
Trip team Family! Great tutorial!
New to the family 👪 really enjoying all your videos. Cheers keep it up..💯💣
Yo Willy. Some cheap lighter I had malfunctioned and almost burnt down my bathroom. all the black soot I was cleaning up the other day gave me the same idea!! Im gathering all the info I can right now. I was curious about the step for pressure sterilizing your Mason jar with the agar. Do you leave it loose with foil, it would seal otherwise right?
So do you use agar peitre dish culture to inoculate grain spawn or spore syringes? As in personally? For cloning the agar peitre dish obviously but inoculation if not cloning spore syringe or swab?
HI IM HAPPY TO SE THIS VIDEO . THANKS SO MUCH FOR THE ALL YOUR HARD WORK. From Spain with love
Trip team Family
Ive learned alot from your knowlege thanks alot ( keep on Keepin on )
that hoffman acid poster is so dope
Hi Willy! Do you have a video on how to put spores to an agar dish to grow out the mycelium ? If not, could you please do one!
Many thanks!
I always thought charcoal might work lol... definitely gonna do it I always have it available for when I distil my whiskies
I really love your educational videos. They are very informative and well produced.
Do you ever grow gourmet mushrooms like lions mane? I'm really looking for the best substrate (and ratios) that doesn't involve soyhulls (not available in my area) but isn't just straight up HWFP. The hard wood pellets work fine by themselves but I'm looking for bigger, and preferably organic grows.
Do you have access to wheat bran? 80% hardwood sawdust - 20% wheat bran works.
@ale.ktheo.g4394 Thanks for your response. I appreciate it. I've been growing LM successfully on masters mix for the past several years. My initial comment was from a few years ago. It took a bit of digging, but eventually, I was able to find the soy hulls.
Hey Willy instead of light malt extract and you use Karo Syrup?
Those framed blotter sheets look dope!
Actually got a stack of custom printed blotter.
Would look sick to get a sheet with your name/logo on if.
Got some 1200 squares with a sick illustrated Xanax bar over every 2 squares. Mainly use them for making flualp blotter a load of the bigger blotters which are 1/2" 10x10 squares with logo and branding on them. Perfect sized for holding 2-3mg of product per 'hit'
Can I please get in tune with you? My L connect passed away recently. And I'm running out of gels from my last sheet. 😭
heyheyhey, can black Agar be used to inoculate grains or L.C straight ? or must it first be transferred back to the normal agar mixture than to grain and L.c, thank you, love and light
Tripteam family is the best!!
11 minutes and 36 seconds in.... "alright guys let's get into this video"
Going auto mega in a 4x8 thanks Willy
Trip Team Family 🤜🤛 Mushada here breakin fools through in the Midwest ova heya!!
Trip Team love it
Is there any type we should not use agar with activated carbon?
I saw an article yesterday on making a dung agar for coprophyllic fungi. Can you or should you add the activated carbon to this? Have you tried this?
Hi Willy, I love your content, always informative and detailed for noobs like me. I just wanted to ask "How long should Agar plates be left for drying at Room Temperature before they can be inoculated ?" TIA. Mush Love.
Until they reach room temperature. If your agar doesn't solidifies, you made it the wrong way. It's ment to get solid when cold.
Does this recipe scale up or does the ratio change in large quantities . Any ttf that could help . Mush love all
I seen a guy use regular charcoal on mushrooms not this type but it seem to work well he had a control to compare to also.
With glass petri dishes, can I put those in the pressure cooker with agar in them already?
Damn, I’m glad I found you. Subscribed
Thank you for all you do!!🌈♥️🌈🍄🍄
What are some other good agar recipes you personally use?
Damn I am gonna be a neighbor soon. High Five!
How long can I keep my agar dish with mycelium in it before I need to use it or dispose it will it stay fresh
Yoo great video i just dropped some old spores on regular mae agar i wish i found this tek before spores are taking some time
Condiment containers work well as agar dishes too. Relatively sterile (I've never seen more than a 12% contamination rate with them)
Trip Team Family. Mush love
Is activated charcoal the same as activated carbon?
Great video, thanks for sharing your knowledge :)
Cool. Thank you
cool video very detailed and thorough thank you sir
what is the benefits of adding activated carbon ??
Thanx for info ;) Keep on Trippin
Could you do a full video on saving genetics? Like from Syringe to infinite spores? It would be nice to have one conclusive video on how to only buy one syringe per strain.
I second this.
@@Jayaditya_shrivastava 3rd
I'll 4th it, but will also comment that when you start from spore, you have billions of possible genetic variations, and this is where you start developing a new *variation* It does NOT preserve a particular isolated culture. Cultures are kept by storing the desired living mycelium long term, in refrigerated storage, generally in slants. When starting from spore, there is a process to isolate mycelium that is fast growing, free of ANY contamination, and RESISTANT to contamination. This process requires one to start from a spore print (that is quite prone to contamination) then transferring from one agar plate to another to eliminate contamination as well as to select for they type of growth that indicates THAT type of mycelium shows desirable traits, such as strong rhizomorphic or tomentose growth, depending on the species. Then you do more sectioning and transfers to further "clean up your work."
Once you have a few dozen prospects that look promising, you have to fruit them all to see if any of them perform worth a Tinkers damn - and that's just the first round. The video COULD take a few years just to put together the first part, if you want a real step by step walk-through from spore to vigorous, strong strain that will also develop fruiting bodies that have all the characteristics you are after.
This is why many just start by doing multi-spore in grain --> selecting a fruit (or several) that show desirable traits from the first few fruiting blocks, then just taking live tissue cultures from those specific selections ---> STRAIGHT to agar to clone the fruit body itself.
Going all the way back to spore and starting from there is a lengthy process, so that's not likely to be a single video. There's more to it than meets the eye at first, but once you start to understand, it's less of a learning curve. It's very different than selecting varieties and cross-breading or grafting them in the plant kingdom, but also sort of similar in many ways.
For LC agave nectar instead of honey?
Will jar explode if we don’t punch a hole on the lid & cover with tape, when sterilizing in pressure cooker. ✌️
Not if it’s not fully closed
tnx willy your awsome im always come to you for confirmation plz plz do one on extractions
I heard you can put black agar it in your grow room and test for air quality like legionnaires disease.
Trip team family
Greetings from sunny SW Florida. Is activated charcoal capsules a ok substitute please? Thanks
Did u ever explain the reason for using activated carbon?
Willy why didn't you mention ketchup cups??? They're only the cheapest and reliable way to go for beginners
Ketchup cups?
Trip Team Family!!! ☮️😊
@ALX 8721 ketchup cups; top snaps on easy secure and no parafilm struggling to get it on! Yupppp
@ALX 8721 Gotta try them, holmes. They're a whole *lot* cheaper, and a whole lot DEEPER! If you pour ketchup cups deeper for long term storage, they actually do also serve as "Not quite a slant - but definitely don't dry out as fast as plates!" They're smaller so take up WAY less room. They don't crack if handled rough during shipping. They're available in ALL local restaurant supply places, or in smaller quantities at just about any major grocery.
If someone's doing *ONLY* sectoring on agar (to develop new genetic expressions and/or crosses), then yeah, ketchup cups are going to be of somewhat limited use because of their size, but they still have their use even there if you really think about it. It's a great way to save cultures to work with at a later time without having to spend hundreds on 15 or 30 mL tubes - and tube racks. Just pour 30 mL ketchup cups with about 20 mL of agar in each, and rock arm. 20 mL of agar with some depth IS a sort of "Intermediate slant on the cheap" as far as how long it will support living mycelium. It'll like support a culture for 9-12 months pretty easily.
As for simply quality testing of liquid culture OR spawn (if your are a commercial grower), ketchup cups are AWESOME! Every L.C. run should have 2 or 3 samples transferred to agar BEFORE starting to use it to inoculate anything. That's not to say, "Don't use the L.C. until it's been grown out on agar." Best practices seem to be, however, to take the very first "pull" of L.C. mycelium to agar, THEN start making syringes/nocing up grain (or sawdust or more liquid or whatever). By regularly growing samples at the beginning of a L.C. jar, you will KNOW if your bulk spawn (or whatever you noced up with that particular jar) is contaminated, because your cups will show it too - WITHOUT having to "plate up a sample when tams are suspected," then WAIT and SEE.
I hope you try them, even though you said you "PROBABLY" wouldn't. :) You can get like 50 of 'em with lids for 3 or 4 bux at the grocery store. Bulk 100x15, 90x15 or even 60x15mm plates can not be had at nearly the same price as VERY cheap ketchup cups, which can trim the fat out of the budget, not only for those that are just trying their hand at mycology just to see if it holds their interest, but for those that are producing huge amounts of L.C. or doing huge numbers of grain to grain transfers for any size commercial op, big OR small.
I've appreciated your comments, and hope mine to you on this subject sways you to splurge on yourself, and pour like, (100) agar cups (which are cheap AF if you buy 'em in bulk especially) nice and deep with 20 mL each. It'd be a cool way to try, for example, a known and proven LQ sample on:
- a few carbon/LME "plates" (cups?)
-VS-
- a few carbon/potato cups
-VS-
a few each of cups using agars WITHOUT carbon
This is just ONE such use where, you're not really needing a large surface area (for sectoring a large area of growth)
Slides ketchup cups closer to ALX.
Join the dark side, @ALX 8721. Become one of those "Ketchup Cup People." Join us. You'll love it! heh It's almost Halloween you know. I'm a spooky hob-goblin. We are infiltrating your brain. Let us IN! :)
@ALX 8721 I'd hazard to guess that 5,000 cups are about the same or even less moneythan to 500 plates, and can be bought from a restaurant supply place right in our home town. I really do hope you give it a whirl for at least for "semi-long term" storage, partly because tubes and racks for slants are WAY more than 10x the cost of snap-lid cups, so I think you'll be pleased - but mostly - because I am eating your brain, and this is how we Zombies start. Just a little nibble. Just try it for, "Cultures you DO want to work with in the next 2-3 months, but just can't fit them into your work-flow with other stuff 'right now.' " {Mwwwwwhaha-HAAAAHAHAHA}
RA RA! KETCHUP CUP LOVE!
RA RA! KETCHUP MUSH LOVE!
The cup-lovers are going to eat your brain!
[/soapbox]
Benefit of activated carban: activate growth with mycelium faster, black agar with germinated growth shows better with transfer. And shows infescted agar dishes better.
Saved you 26 min
But willys world has taught 30% more about mushrooms than I know so stayed tuned with this guy
Thankyou bro...I'm sorry I can't watch his videos them shits be too long to say something I can't do it
Nice one.... Can we use food coloring as well, to make colors?
Yes
hi willy 🤙🏽 can you tell whats the reason to sterilize the agar in another container instead of pouring it on petris and then sterilize ? wouldnt it be less prone to contam ? Sorry if my question is too noob 😅
most petris and parafilms aren't heat rated and will melt under pressure and that also sounds gratuitous and messy.
What do you mean by lookin for the sectioning? Thanks✌️🍄
Trip team family. Smoked out with Tommy and dying to run across him again, he is such a blast.
What
Trip team family!!! Gimme dat yesca!!!
Do you pour the agar when it’s very hot or you wait? If so what is the ideal temperature to pour it into the dish?
Around 140 F