How to interpret the results of proteomics data analysis? Proteomics Data interpretation
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- Опубліковано 22 сер 2024
- #howtointerpret #proteomics #interpretation
In this video, I have explained how we interpret the data proteomics from basic, and provided a practical guide on how the conclusion from figures and visualizations can be written well.
Review article: A practical guide to interpreting and generating bottom-up proteomics data visualizations
DOI: 10.1002/pmic.202100103
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In fact, SILAC, TMT, and other methods use stable (non-radioactive ) isotopes with 13C and 15N en.wikipedia.org/wiki/Stable_isotope_labeling_by_amino_acids_in_cell_culture (5:17 min)
Sorry what i mean is the use of isotopes
Thank you so much for this helpful video. Please is there a book allow me to understand these figures and how to create them??
There is no book but review article you can find online
Sir, is it possible to use volcano plot for proteins obtained in a single set of control and treatment whole cell proteomics data
Its possible
Nice 👍🏻
Glad you like it
Thank you a lot for your videos! :) It would be nice if you can make an interpretation of RNA seq and ChIP data soon. :)
Thanks, Teodora, Please note RNA seq data interpretation videos are already uploaded on my channel.
How to calculate FC, log2FC, Pvalue, Padj, Up/down genes in RNA seq data using Excel
ua-cam.com/video/HH3Mll4W5WE/v-deo.html
How to find genes of interest in RNA-seq / transcriptomic data ? Kegg pathway
ua-cam.com/video/LeNjskbF8RY/v-deo.html
Interpretation of Principal component analysis (PCA) in RNA seq
ua-cam.com/video/aUOV7EBGOy8/v-deo.html
How to select genes for qPCR validation in transcriptome/RNA seq data?
ua-cam.com/video/PRhHBcjKAKU/v-deo.html
How to interpret the results of KEGG pathway analysis?
ua-cam.com/video/meTGfLVBiRs/v-deo.html
How to write and interpret gene ontology (GO) in research articles ?
ua-cam.com/video/kRt2IWfhCqU/v-deo.html
Interpretation of RNA-seq heat maps
ua-cam.com/video/cRT5mpGMDmQ/v-deo.html
Interpretation of RNA seq data | DEGs | GO | KEGG
ua-cam.com/video/xc2zEI_5pmw/v-deo.html
You can watch them in reverse order, last one first and watch one by one while moving up. Hope you will get answers.
Salam. Is there a certain website for proteomics data analysis? These figures are very nice but seems like they are using Phyton programming to generate them. Thank you
Yes there are websites, and these figures are generated through python. Their code are available (on Git Hub) and free to use.
@@asifmolbio Can you share the websites please? I know ShinyGo from you. I have no experience in Phyton
Thank you. Sir any tutorial video about MxQuant software for phospho data?
Thanks saboor, i have noted down your suggestion. Please stay tuned.
Sir, My question is how to analyse protein data? is there any platform like iDEP where we can analyse the proteomics data? Kindly guide me regarding that. Thank you.
Yes there are but its paid, but differential analysis can be done freely
@@asifmolbio Sir, Kindly make a video on how to analyze protein as well.
How to do differential analysis free for protein?
thank you for your wonderful videos,..sir, please do one video for metabolomics data
Soon inshaAllah , stay in touch
Thank you for the information. May I have your email id, please. I have several queries regarding proteomics analysis.
asifalikalas@foxmail.com