Bioprinting 101: How to make Microfluidic Chips

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КОМЕНТАРІ • 47

  • @rock3tcatU233
    @rock3tcatU233 3 роки тому +8

    Is it advisable to use a vacuum pump in order to quickly remove the air bubbles in the PDMS?

    • @erwinz5926
      @erwinz5926 2 роки тому +1

      looks like they used one. propably it is. i thought the very same watching this.

  • @danielayala646
    @danielayala646 5 років тому +6

    cool! how many more times can the printed design be casted? or doesit disinttegrate aftyter first cast?

    • @SE3Dedu
      @SE3Dedu  5 років тому +4

      It's one print per cast. Once you cast, it solidifies over time. Then you can wash off the channels.

  • @khaledfreij6586
    @khaledfreij6586 5 років тому +5

    Can i have more info about the dimensions of the channels?

  • @maxfreudenstein6892
    @maxfreudenstein6892 3 роки тому +4

    The music gives me hope that som day our peple shall be free again

  • @weitingLin66
    @weitingLin66 5 років тому +4

    nice introduction! make it pretty clear

    • @mayasarilim3611
      @mayasarilim3611 5 років тому

      Thank you for the feedback. Glad this was useful.

  • @RuyRuiz
    @RuyRuiz Рік тому +1

    Love it, omg you really make sounds so easy. New Subscriber.

  • @carlosvelazquez6440
    @carlosvelazquez6440 3 роки тому +2

    Man, I'm just trying to figure out how colors mix to produce different colors, like is it linked to turbulance and if so is it a chemical reaction, or an optical illusion.

    • @theglobalwarming6081
      @theglobalwarming6081 3 роки тому +1

      The color is based on the pH of the fluid. I think they used one universal pH indicator for both samples. So, when they change color, they change due to a chemical reaction.

  • @kryogenica4759
    @kryogenica4759 5 років тому +2

    Are the channels exposed to the open air on the bottom face?

    • @SE3Dedu
      @SE3Dedu  5 років тому +1

      Yes we use tape to cover the channels so you have a seal to flow fluids through. Otherwise you can use a glass slide.

    • @kryogenica4759
      @kryogenica4759 5 років тому

      @@SE3Dedu Thank you so much for the info! Will see if these can be implemented in a classroom.

    • @milascatadventures1011
      @milascatadventures1011 5 років тому +2

      @@kryogenica4759 If you go to portal.se3d.com and sign up for a free account, you can find our print files and instructions there, under Additional Resources, Bioprinting Project Kits.

  • @visionbios6736
    @visionbios6736 4 роки тому +1

    Any recommendations on how to attach the PDMS device onto a glass slide ?
    Any help is much appreciated

    • @mayasarilim3611
      @mayasarilim3611 4 роки тому

      Just make sure both surfaces are clean and dry before attaching the glass slide onto the PDMS cast. Good luck!

    • @Amyhb69
      @Amyhb69 3 роки тому +1

      Plasma

    • @sharadambardar9252
      @sharadambardar9252 2 роки тому

      oxygen plasma treatment

    • @bhatt6873
      @bhatt6873 2 роки тому +1

      You have to go for O2 plasma etching of the glass slide after which you bond it with the PDMS

  • @aslcatalyst416
    @aslcatalyst416 5 років тому +1

    Hi is the CAD for this part available for download?

    • @SE3Dedu
      @SE3Dedu  5 років тому +1

      Pls visit portal.se3d.com and sign up for free to access files, under Additional Resources, you will find it under Bioprinting Project Files

    • @baltakatei
      @baltakatei 2 роки тому +1

      @@SE3Dedu lol, it's dead.

  • @amitasthana45
    @amitasthana45 Рік тому +1

    can you please send us the dimension or STL file to me

  • @zakyrayadhirh
    @zakyrayadhirh 4 роки тому

    can I get a paper from your research?

  • @a.n.9227
    @a.n.9227 4 роки тому +1

    Hi can you send me a quotation of prices of the reagents you used? PDMS, Fpluronic, ... or even redirect me to someone who could do so. thanks a lot

  • @nebuchadnezzar47
    @nebuchadnezzar47 4 роки тому +1

    why dont they just completly 3D print the end product in plastic? with channels built in?

    • @TheMrJuoji
      @TheMrJuoji 4 роки тому

      depending on the kind, but fdm is out because of the porosity , i don't know for resin printing but i guess you'd need something chemically inert , as for slm/sls, it's probably feasible yet would be an expensive piece of equipment

    • @ignacioaguirrenoguez6218
      @ignacioaguirrenoguez6218 Рік тому

      They are doing that nowadays

  • @lintong8703
    @lintong8703 Рік тому

    Pluronic 127 for creating the mold / channels

  • @PrasannaKumar-zx7gr
    @PrasannaKumar-zx7gr 5 років тому +1

    Really interesting

  • @Muradsahar
    @Muradsahar 3 роки тому

    Amazing!

  • @Farhan-co9he
    @Farhan-co9he 2 роки тому

    impressive

  • @vivianaclaveria9123
    @vivianaclaveria9123 4 роки тому +1

    This is not microfluidics. This is millifluidics. Nice video and technique anyway.

    • @SE3Dedu
      @SE3Dedu  4 роки тому +1

      Yes you are absolutely correct. More for demo and educational purposes.

  • @pubgamedz
    @pubgamedz 2 роки тому

    Whey i Can't download! 🛸

  • @erwinz5926
    @erwinz5926 2 роки тому

    so- the result is green yellow!!!11! *scnr.* : )))

  • @PrasannaKumar-zx7gr
    @PrasannaKumar-zx7gr 5 років тому +1

    👍

  • @Karpe_Deem
    @Karpe_Deem 3 роки тому +3

    3:44 minutes for a fraction of a second of demonstration 😢

  • @maracachucho8701
    @maracachucho8701 5 років тому +2

    Not very "micro", though!

  • @miron__
    @miron__ 5 років тому +3

    fix your audio

  • @wirehyperspace
    @wirehyperspace 7 місяців тому

    Lego land

  • @georgen9755
    @georgen9755 Рік тому

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  • @georgen9755
    @georgen9755 Рік тому

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  • @mdgar6516
    @mdgar6516 4 роки тому

    quit the music, it is irritating