Hi Adwoa, thank you for the helpful video. I am trying to test multiple conditions so I need different blanks on the same plate, but I can't seem to find how I can add more than 1 blank. Do you have any idea how that can be done?
Hi. You could simply treat your additional blanks as unknowns, and subtract it from the background of the corresponding condition. I am not aware of how to do it automatically with the plate reader software. Good luck.
Hi Adwoa, what do you do when you a running a luminescent assay at 2 different wavelength ranges ? I chose a dual pmt but i’m unable to get the exact wavelength since it’s a filter based approach on Gen5
Hi Robert: Wavelength is a range, so pick the value that includes the wavelength range you're after. For example, for red colour, the wavelength is between 620-750nm, so you can pick a filter that spans this range. Orange colour is 590-620nm; Yellow is 570 - 590nm; Green is 495 - 570nm etc.
@@robertdevine5287 You can also see my video on AlphaScreen Assays where at 4:46, I show the colour chart and thier corresponding wavelengths: ua-cam.com/video/l_IqhZqX9s4/v-deo.html
The purchaser of the machine at your institute will have a CD with the software. The software allows 5 installations (5 licenses) so track them down and see if there is any remaining for you to install. All the best.
@@adwoabiotech Hello. I followed your video on the gen5 manual but unfortunately I did not find the protocols in my installation folder. Can you help me? thank you
Subscribe for a fun approach to learning lab techniques: ua-cam.com/channels/4tG1ePXry9q818RTmfPPfg.html
Hi Adwoa, thank you for the helpful video. I am trying to test multiple conditions so I need different blanks on the same plate, but I can't seem to find how I can add more than 1 blank. Do you have any idea how that can be done?
Hi. You could simply treat your additional blanks as unknowns, and subtract it from the background of the corresponding condition. I am not aware of how to do it automatically with the plate reader software. Good luck.
How do you adjust the gain?
How to setup time to read a wavelength in every 2 minutes?
Hi Yas. You will need to use the 'Kinetic' option when setting up the list of steps in the procedure. Please see 1:54
Hi Adwoa, what do you do when you a running a luminescent assay at 2 different wavelength ranges ? I chose a dual pmt but i’m unable to get the exact wavelength since it’s a filter based approach on Gen5
I will be glad if you can help
Hi Robert: Wavelength is a range, so pick the value that includes the wavelength range you're after. For example, for red colour, the wavelength is between 620-750nm, so you can pick a filter that spans this range. Orange colour is 590-620nm; Yellow is 570 - 590nm; Green is 495 - 570nm etc.
@@robertdevine5287 You can also see my video on AlphaScreen Assays where at 4:46, I show the colour chart and thier corresponding wavelengths: ua-cam.com/video/l_IqhZqX9s4/v-deo.html
good video, how I can get Gen5 software ?
The purchaser of the machine at your institute will have a CD with the software. The software allows 5 installations (5 licenses) so track them down and see if there is any remaining for you to install. All the best.
@@adwoabiotech Hello. I followed your video on the gen5 manual but unfortunately I did not find the protocols in my installation folder. Can you help me? thank you
What is machine cost
Around $40K as of 2023
Okay
you talk too fast
Noted.
You can also slow down the speed using Settings, then Play Back Speed option.