Thanks a lot for the very detailed explanation! In my case, I found a stop codon in the middle of the sequence of my gene 😥 it's a very conserved gene so I'm looking for ways to verify and hopefully correct the assembly and annotation.
Hi, think I've had a few like that. I also checked gene sequences against those in other strains or species to see if there was a difference in my sequence alone. Also had to check sequencing coverage for the region to check for assembly error caused by low coverage etc...had some gene clusters riddled with errors caused by this. Hope you get it all sorted out. BR GenomePJs
Thanks a lot for the very detailed explanation! In my case, I found a stop codon in the middle of the sequence of my gene 😥 it's a very conserved gene so I'm looking for ways to verify and hopefully correct the assembly and annotation.
Hi, think I've had a few like that. I also checked gene sequences against those in other strains or species to see if there was a difference in my sequence alone. Also had to check sequencing coverage for the region to check for assembly error caused by low coverage etc...had some gene clusters riddled with errors caused by this. Hope you get it all sorted out. BR GenomePJs