Plasmid Design - Promoters

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  • Опубліковано 22 гру 2024

КОМЕНТАРІ • 15

  • @Danishalig-v9j
    @Danishalig-v9j 15 днів тому

    Hey, thank you very much for your series. Really you save me. I was thinking i chose wrong career but your video gave me another hope. I really wants to say thank you very much for such great work. You saved thousand of beginners.

  • @gregsmith6756
    @gregsmith6756 2 роки тому +3

    This is really neat. I am determined to learn this molecular biology. Thanks for the upload.

  • @user-ce4li4fj6h
    @user-ce4li4fj6h 2 місяці тому

    Thank you very much for your videos! They are very well structured and helpful (from a chemist who learns bio)

  • @laurarojas3283
    @laurarojas3283 Рік тому

    Muchas gracias profesora por explicar de forma tan clara. You're the best

  • @axospyeyes281
    @axospyeyes281 2 роки тому +4

    Hey, loved this series about plasmid design. I didn't quite grasp the concept of eukaryote promoters though... Could you maybe make a seperate more in depth video about those? would be greatly appreciated, coming from an aspiring biologist!

    • @katharinehubbard5043
      @katharinehubbard5043  2 роки тому +2

      Thanks - eukaryotic promoters are on my list to do!

    • @ashwinnair4816
      @ashwinnair4816 2 роки тому

      @@katharinehubbard5043 thankss

    • @zixinleaf6499
      @zixinleaf6499 Рік тому

      so do I, I think the concept of the eukaryote promoters is difficult for me. Such many upstream factors. I want to learn more detailed information, Thanks!

  • @abumohammed9659
    @abumohammed9659 10 місяців тому

    Please keep going you are gorgeous. Many thanks goes to to from form UEA Norwich!!!

  • @abdoulayeassane2852
    @abdoulayeassane2852 Рік тому

    How about promoter for viruses?

    • @katharinehubbard5043
      @katharinehubbard5043  Рік тому

      Viruses are a lot more complicated! Some viral genes are expressed using a viral RNA polymerase, while other viruses exploit their host cells (either bacterial or eukaryotic) and express their genes using a host polymerase. It depends what virus you are interested as how it's promoter works/is structured. There is therefore a lot of variation in bacterial promoter structure and more than I can easily capture in a 15min video!

    • @abdoulayeassane2852
      @abdoulayeassane2852 Рік тому

      @@katharinehubbard5043 Thanks for your kind feedback. Let's say "cassava mosaic virus". It will be highly appreciated If you could share some good references with us, Thanks in advance

    • @katharinehubbard5043
      @katharinehubbard5043  Рік тому

      @@abdoulayeassane2852 I've not worked on Cassava Mosaic Virus so am not an expert here! However, Cassava Mosaic Virus is a geminivirus which has a single stranded DNA genome. Like a lot of DNA viruses, geminiviruses don't encode their own RNA polymerase, but rely on the host (eukaryotic) polymerase for transcription. Because the genes are transcribed with a eukaryotic nuclear RNA polymerase, they have a similar promoter structure to eukaryotic genes - Figure 4 of this paper shows the presence of TATA box motifs in regulatory regions. Hope that helps! www.ncbi.nlm.nih.gov/pmc/articles/PMC6640248/

    • @abdoulayeassane2852
      @abdoulayeassane2852 Рік тому

      @@katharinehubbard5043 Thanks for your kind feedback

  • @overlander2771
    @overlander2771 Рік тому

    I love you!