May I have a question ,if the cDNA is from the eukaryotic organism ,Do we need to use the pcr primer to remove the RBS and the start condon first . As the bacterial ca not use the
Hello, brother. I speak Arabic, can I get a pdf On the email ? Hello, brother. A nice explanation I speak Arabic can I get a pdf for the purpose ofTranslation
It's artificially provided in the vector because it helps recruit the ribosome to the mRNA to initiate protein synthesis by aligning the ribosome with the start codon. Goal of an expression vector is to express the protein in bacteria/ eukaryotic cell.
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Most of them are shuttle vectors such that can be used in prokaryotes And eukaryotes at the same time... hence they have two origin...further watch my videos on shuttle vector
You have to clone the promoter sequence along with your target gene and then put it in the vector. In general expression vector already has promoter sequence
Very interesting question, this should be a video on it's own. In short, Linkers and adaptors can be used which are oligonucleotides that are useful in DNA ligation during blunt end cloning. In cloning experiments the cleaved DNA often has blunt or sticky ends. Sticky ends are easy to ligate but DNA strands with blunt ends are hard to ligate. The linker and adaptor molecules then come into play. The linker and adapter molecules have both blunt and sticky ends and internal restriction sites as well, which help in DNA ligation.
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That’s a gateway cloning vector .... actually gateway cloning is much better than the restriction ligation based approach.... efficiency is much higher
This video was very informative and incredibly helpful. Thank you for breaking down each component and the significance/importance of its presence.
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I understood more in this 6 minutes than my 16 week biochem course
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Thank you 🙏
Aysha Ayshaa please share among your friends
@@animatedbiologywitharpan sure , I will ✌
dude i searched Wich restriction enzymes are required to clone the mammalian expression vector pMV2 BMPR?
a there is no game question
May I have a question ,if the cDNA is from the eukaryotic organism ,Do we need to use the pcr primer to remove the RBS and the start condon first . As the bacterial ca not use the
Hello, brother. I speak Arabic, can I get a pdf On the email ? Hello, brother. A nice explanation I speak Arabic can I get a pdf for the purpose ofTranslation
shin dalgarno sequence found in prokaryotic mRNA..
It's artificially provided in the vector because it helps recruit the ribosome to the mRNA to initiate protein synthesis by aligning the ribosome with the start codon. Goal of an expression vector is to express the protein in bacteria/ eukaryotic cell.
Thanks you so much. Your videos are a life saver to understand complex topics. Really appreciate your efforts
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@@animatedbiologywitharpan sure I will
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Hatts off to you man, you just summarized the whole chapter for me. Liked you video💗
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Expression vectors
Protein purification
ua-cam.com/video/rbcDxuc8TOw/v-deo.htmlsi=Weigqs3xCsfb0McK protein purification
Can you please explain why Expression Vectors like BL21DE3 are poor choice for direct Cloning.
Very well explained
Thanks
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Nicely summarized, thank you 👍
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Where is gateway cloning video?
very informative. Keep making videos :)
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Plz tell me is the eukaryotic gene of interest only expressed through expression vector or prokaryotic gene also could be expressed?
Both eukaryotic and prokaryotic genes can be expressed using the expression vectors but the type of expression vector is different.
U beauty sir simple and precise
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Very nice video for understanding the expression vector 👍
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Hi, can you explain about vector that has two origin of replication? why it is so?
Most of them are shuttle vectors such that can be used in prokaryotes And eukaryotes at the same time... hence they have two origin...further watch my videos on shuttle vector
yeahhh i just found your video on shuttle vector, thank youuu
Fizha Adnan good
Thanks for info. You need a better mic
Amazing video.
If want to add promoter what to do ?
You have to clone the promoter sequence along with your target gene and then put it in the vector. In general expression vector already has promoter sequence
@@animatedbiologywitharpan yes so.. I need to remove that promoter ? And if yes so there is no restriction site, in both side of promoter so how?
Nice work bro!
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Thank you! helps me a lot!
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How to check for gene expression in cancer cells by finding candidate genes?
Rna seq or if you have candidates then you can do qPCR
can you send this ppt
how did you do it can you share with me , thank you
I create everything in PowerPoint
thank uu, i have a question: our insert gene how inserted if they do not have the same blunt nucleotides?
Very interesting question, this should be a video on it's own.
In short, Linkers and adaptors can be used which are oligonucleotides that are useful in DNA ligation during blunt end cloning. In cloning experiments the cleaved DNA often has blunt or sticky ends. Sticky ends are easy to ligate but DNA strands with blunt ends are hard to ligate. The linker and adaptor molecules then come into play.
The linker and adapter molecules have both blunt and sticky ends and internal restriction sites as well, which help in DNA ligation.
In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.
so naturally our insert of interest itself has sticky ends and how do we know which sticky end Bam, Hind or which has?
amaaaazing video,, thank you so much
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Highly informative video.Keep doing more....!
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This specific video is specifically nice also it's specific
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Very helpful! thank you
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Can we use pdest17 for Cloning and expression
That’s a gateway cloning vector .... actually gateway cloning is much better than the restriction ligation based approach.... efficiency is much higher
@@animatedbiologywitharpan thanks. Can we directly insert our gene of interest or do we need to use pentr D TOPO
Thanks man!
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Can expression vector be linear?
The most popular ones are generally circular. There could be some linear phage based vectors but i dont know much about them
Thank you
Better than most! Keep it up!
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Thank u sooooooo much
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Of course.....keep it up..plz
Loved it
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Really helpful
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Dude I can't judge you I am small in means* of information but u have improve ur talking skill👍
I mean I can't hear sometimes clearly