Hi ... there is a UCSC Genome Browser for Prokaryotes as well ... unfortunately it does not have the sequence for Dengue virus ... so may not be possible - however, since prokaryotes are small genomes with low repeat content and single copy genes - if you design a primer pair of18 bases or more it should be specific unless of course you design it to a repeat sequence
Sir I have to do insilico pcr for Dengue virus .So, how can i get it as there is no option for dengue virus genome?
Hi ... there is a UCSC Genome Browser for Prokaryotes as well ... unfortunately it does not have the sequence for Dengue virus ... so may not be possible - however, since prokaryotes are small genomes with low repeat content and single copy genes - if you design a primer pair of18 bases or more it should be specific unless of course you design it to a repeat sequence