I must say this is the best video for pulse field gel electrophoresis on UA-cam. Not a single indian educator explained this technique as beautifully as you did. Thanks
Ohhhhhhhhhh now you got me reminiscing. i used a PFGE set up when i worked in cancer research years ago. The hope was to find a set up that could be scaled up to workout if patient A, B or C needed the *full dose* of cancer treatment or hopefully slightly less. i have black hair if i waned to dye it red i’d have to use one hell of a lot of red dye (probably have to bleach it first then dye it red). Someone with blonde hair would not need the extra step & would need less dye. So if thats true about hair colour, we are all different amounts then it surely must be true about DNA. i was trying to explain all this to a friend so glad to come across this video. Thou not sure you quite described how fiddly the bloody thing was, especially in summer. So i have lovingly made my little tiny squares with human dna. i then zap the poor little squares of gel with x-rays, causing the double strand breaks. Then the little squares of jelly get their own back as using a biology fume hood with your hands up inside & then in that position you had to get each jelly square balanced on the tip of a scapel, sometimes trim it CAREFULLY and place it into the well. Sounds easy until you think (especially in summer) you are trying to fit two bits of jelly together, neatly & accurately You have to be cardful put it in with no mushing it in or breaking the plug. The more you mush it up you are creating more double strand breaks. There were times when....... lol. i just 😶😶😶. Txs for the work you are doing here i might nerd out & have a look around. Is couple of videos showing the lab equipment, my baby, but you certainly explained it best. Thanks for a trip down memory lane.
Great video! Really easy to follow. However, I have a question concerning the switching time parameter: When I want to perform this technique, the system asks me to input two values (for example: initial time 10 sec; final time 30 sec). What exactly mean those two values??
Thank you for your video. But I am confused why do not we just extract and elongate DNA samples outside and put them into the wells? So we do not need to use several currents to elongate them.
Other questions are about the parameter setting: 1. I think a larger angle should be better for resolution since the route can be longer. I do not understand why you said the smaller angle is better. 2. You mentioned that the time and voltage should be the same for turning the right and left direction. But as the DNA elongates, its moving speed will also change. So although you use the same voltage and time, it may still cannot be straight. How to deal with that?
how does PFGE change when doing it in a capillary? because i think that in capillary, there is only one direction possible for the electrical field right?
Or or or, look here. I have an idea. Listen to this, YOU can skip this video and avoid being irritated leaving everyone else to learn in peace. What a concept?!
I must say this is the best video for pulse field gel electrophoresis on UA-cam. Not a single indian educator explained this technique as beautifully as you did. Thanks
Thank you very much for this video! You're an amazing teacher.
Ohhhhhhhhhh now you got me reminiscing. i used a PFGE set up when i worked in cancer research years ago. The hope was to find a set up that could be scaled up to workout if patient A, B or C needed the *full dose* of cancer treatment or hopefully slightly less.
i have black hair if i waned to dye it red i’d have to use one hell of a lot of red dye (probably have to bleach it first then dye it red). Someone with blonde hair would not need the extra step & would need less dye. So if thats true about hair colour, we are all different amounts then it surely must be true about DNA.
i was trying to explain all this to a friend so glad to come across this video.
Thou not sure you quite described how fiddly the bloody thing was, especially in summer. So i have lovingly made my little tiny squares with human dna. i then zap the poor little squares of gel with x-rays, causing the double strand breaks. Then the little squares of jelly get their own back as using a biology fume hood with your hands up inside & then in that position you had to get each jelly square balanced on the tip of a scapel, sometimes trim it CAREFULLY and place it into the well.
Sounds easy until you think (especially in summer) you are trying to fit two bits of jelly together, neatly & accurately You have to be cardful put it in with no mushing it in or breaking the plug. The more you mush it up you are creating more double strand breaks. There were times when....... lol. i just 😶😶😶. Txs for the work you are doing here i might nerd out & have a look around.
Is couple of videos showing the lab equipment, my baby, but you certainly explained it best.
Thanks for a trip down memory lane.
The best explanation.. ver easy to understand
Dear lady i cannot thank you enough. ❤❤❤❤❤
Excellent presentation and well-explained! Thank you!
simplified and throughly explained...
great work..
Thank You
you are welcome :)
The best video of PFGE
very use full video thank you for your sharing
thanks for your good video
Nice video ....easy to understand thank you for sharing this video to us
Excellent explanation , lucid too. thank you
Great video! Really easy to follow. However, I have a question concerning the switching time parameter: When I want to perform this technique, the system asks me to input two values (for example: initial time 10 sec; final time 30 sec). What exactly mean those two values??
VERY CLEAR EXPLANATION, THANK YOU!
Thank you ... stay tuned :)
Nice video. Thank you
Thank you so it helps me to understand much better 😍
Keep making vedio
Thank you very much! Excellent explanation
Brilliant brilliant explanation thank youuu
Thank you for your video. But I am confused why do not we just extract and elongate DNA samples outside and put them into the wells? So we do not need to use several currents to elongate them.
Other questions are about the parameter setting:
1. I think a larger angle should be better for resolution since the route can be longer. I do not understand why you said the smaller angle is better.
2. You mentioned that the time and voltage should be the same for turning the right and left direction. But as the DNA elongates, its moving speed will also change. So although you use the same voltage and time, it may still cannot be straight. How to deal with that?
great video and great explanation
nossa, amei o vídeo, só lendo o artigo não deu pra sacar. ótima explicação!! Obrigada
awesome video keep uploading with explanation
That's very helpful for me,thank u very much~
Great mam 👍
Thank you...
God bless you
Perfectly cleared
lovely explanation!! thank u so much for this!
good work, so helpful, THANKS!
excellently explained !
Outstanding your teach
Wow amazing mam , pls make video on NMR
Thank you very much very helpful vedio thanku
You really helped me
Thank you so much 👍
Yhank you.....It was well explained
you are welcome :)
I like ur accent 🥰
Well explained! tnx
how does PFGE change when doing it in a capillary? because i think that in capillary, there is only one direction possible for the electrical field right?
thanks
Thank you so much...
very informative
thank you.
Thank you for the awesome explanation...can you make video on SELDI procedure too?
Nice video 👍
Mam please make the video on ftclp
That was useful, thank you!
I know Im kinda randomly asking but do anyone know of a good site to watch newly released series online ?
@Anson Rey I watch on Flixzone. You can find it by googling :)
@Anson Rey Try Flixzone. Just google for it =)
@Anson Rey Try Flixzone. You can find it by googling =)
@Kamryn Finn yea, I've been using FlixZone for since march myself :D
Great video, very useful review
Thank you for your comment ... stay tuned :)
Thanks💗
Thank u
love your accent:)
Can you make a video on column chromatography, circular dichroism, fluorescence spectroscopy and x-ray crystallography please?
I am working on the chromatography videos right now, then I will try to work on the other topics ... thank you for your comments :)
🙏🙏🙏
hi ...amazing video really helped. how can i get in touch with you. ?
I think when I listen to your voice how you look like
Fast protein liquid chromatography
Please stop tongue smacking and your 'errr'. Quite irritating to hear.
Or or or, look here.
I have an idea.
Listen to this, YOU can skip this video and avoid being irritated leaving everyone else to learn in peace.
What a concept?!
@@MegaMie77 agreed
thanks