Analysis of Hydroxymethylfurfural (HMF) in Honey

A step-by-step guide to measuring HMF in honey using a reflectometer, RQflex. HMF content is a factor in determining the freshness of a honey sample. Full information on this analytical method is available here: ms.spr.ly/6058b... Other application notes: ms.spr.ly/6059b...

[@MrALJawder]

Do you mean the whole mix should be 10 ml or 2.5 gram honey +10 ml distilled water

[@yevhendenysenko]

Hello
1) Is it possible to do research in apple or orange juice, or in jams? You only have a technique for elderberry juice. How to do research in this case?
2) And if the juice is light in color? Is it necessary to perform a lightening procedure using polyvinylpolypyrrolidone?
Thank you

[@MerckLifeScience]

The Reflectoquant Hydroxymethylfurfural (HMF) test, item 117952, is suitable for use in juices, fruit juice concentrates, and jams. For optimal results in your particular application, we suggest following the test instructions provided with the test kit.
We also suggest verifying the HMF content by employing a reference method to validate the test results. In the event of discrepancies between the results and those of your reference method, we recommend consulting the application note and decolorizing the sample using polyvinylpolypyrrolidone.

[@sokolaliji4012]

It is the same Method for Honey Powder too? Can you explain please?

[@MerckLifeScience]

@sokolaliji4012 We've developed a specific app note for determining the HMF content in honey using the Reflectoquant Hydroxymethylfurfural (HMF) test: ms.spr.ly/6054Ynhvw (please make sure the correct country is selected in the top right corner.)
Please note that while we haven't verified the method for use with other honey products, we'd recommend the same approach for honey powder. We highly recommend measuring the HMF content using a reference method to validate item 117952 Reflectoquant Hydroxymethylfurfural (HMF) test for the specific application of honey powder.

[@DoerteLaue]

How sensitive is the test? What is the minimum concentration of 5-HMF needed in the solution?

[@MerckLifeScience]

The Reflectoquant Hydroxymethylfurfural (HMF) Test has a measuring range of 1.0-60.0 mg/L of hydroxymethylfurfural (HMF).

[@karyguadalupeleonlanderos4979]

@@MerckLifeScience y la lectura del blanco no es necesaria, por que en su protocolo dice que se mida con un tira blanca, pero en el video no lo hicieron, para luego hacer un calculo de conversión de mg/ml a mg/kg?

[@MerckLifeScience]

Determining the blank value of honey depends on its color. For repetitive measurements of the same type of honey it's sufficient to determine the blank value once. In general, it's advisable to take the blank value into consideration even when light-colored types of honey are being investigated for the first time.
With regard to the conversion from mg/ml to mg/kg, please note that the calculation is required because the honey is dissolved in water. However, usually you give a statement of HMF content in mg/kg, so the protocol provides the required calculation scheme from mg/ml to mg/kg.
Hope this helps! Please let us know if you have further questions.

[@hendrajaya.8983]

Hi sir how want to buy this product

[@MerckLifeScience]

Hi Hendra, thank you for your comment! To purchase our Reflectometer RQflex 20, please visit: ms.spr.ly/6050jVlWo. (Be sure to check that the appropriate country in the upper right corner is selected.) Thank you and please let us know if you have any questions!

[@annechaurand8632]

Go to this page and fill the form, a sales person will contact you www.sigmaaldrich.com/DE/en/campaigns/spectroquant-prove-information

[@عمرومجدى-ي4ث]

شكرا

[@MerissaSettles-w7e]

Hilll Brooks