Hi, thanks for the videos. Really enjoy them. We mostly isolate PBMCs for toxicity screening, cytokine production (Th1/Th2) and intracellular activity against mycobacteria. If I'm given a choice between PBMCs and other cell cultures, I would mostly opt for the PBMCs. Much faster and sometimes easier to translate into in vivo studies. 😀
If I'm starting with blood that has had the plasma removed do I add PBS in a ratio of 1:1 to the original amount of blood (PRIOR) to the plasma removed or 1:1 to the volume of blood remaining (AFTER) the plasma is removed?
After plasma removal, what remains is the cellular component of blood, which includes red blood cells (RBCs), white blood cells (WBCs), platelets, and PBMCs (which are mostly lymphocytes and monocytes). The dilution of these cells with PBS can still help in maintaining a proper density difference for the gradient separation. So, go ahead and dilute based the current volume.
The PBMC isolation method was first introduced in 1968 by Boyum: pubmed.ncbi.nlm.nih.gov/4179068/
Hi, thanks for the videos. Really enjoy them.
We mostly isolate PBMCs for toxicity screening, cytokine production (Th1/Th2) and intracellular activity against mycobacteria. If I'm given a choice between PBMCs and other cell cultures, I would mostly opt for the PBMCs. Much faster and sometimes easier to translate into in vivo studies. 😀
It's a pleasure making these videos. PBMCs for the win then! What other cells lineages might researchers use?
If I'm starting with blood that has had the plasma removed do I add PBS in a ratio of 1:1 to the original amount of blood (PRIOR) to the plasma removed or 1:1 to the volume of blood remaining (AFTER) the plasma is removed?
After plasma removal, what remains is the cellular component of blood, which includes red blood cells (RBCs), white blood cells (WBCs), platelets, and PBMCs (which are mostly lymphocytes and monocytes). The dilution of these cells with PBS can still help in maintaining a proper density difference for the gradient separation. So, go ahead and dilute based the current volume.
PBMC will be used to assess viral infections, thanks.
Amazing.
Isolation of CTCs are also another popular use for SepMate/Leucosep tubes.
Good to know, but what does CTC stand for in this context? Thanks.
@@adwoabiotech circulating tumor cells. A popular prognostic biomarker. Look into cellsearch assay.
@@adwoabiotech Circulating Tumor Cell.
Hi! What temperature do you usually spin the sample at? Room? 4ºC? Thank you so much!
Room temp is recommended.
@@adwoabiotech Thank you!