In PCR there are 3 cycles which combined take up some time to generate the daughter strands. In LAMP the synthesis continues constantly.. further: using 6 primers in case of LAMP, provides more contacts for polymerase recruitment!
Heyy, I did LAMP today. The experimental setup was pretty straightforward. BUT, trying to understand the principles behind it, as concisely explained in your video is quite a challenge for me to wrap my head around it. I'll have to do more reading for sure.. 😂 Thanks for the explanation!
I read that most DNA denatured at 75 degrees, does the DNA denatured at 60-65 degrees in LAMP or just some location? Can this become a limitation of LAMP?
The animation is very helpful. It will be even better if was shown at least one cycle after the annealing of external primers, indicated in 3:14. Unfortunately, I live in Brazil and my financial situation is not so good as I would like, but your animations deserve much more than $ 2.00. And thank you for answering my questions!
@@mariociencia12 Why do you donate, when your financial situation is not so good? I am extremely thankful, but I have to make that clear: I would not want anyone to donate if they have to keep an eye on their money!! Thanks anyways :) *And you´re right - I spared myself the animation of the cycle
@@henrikslab I would like to donate more! You deserve! But the donation that I did will do not compromise me. Do not worry. I also did it as an example, hoping that other viewers do the same
Thanks for the work that you put in. Hit me a massage if you like. I am a master in biochemistry and molecular biology maybe we can arrange something together.
This is the clearest explanation I've found. THANK YOU!!
This was giving me a migraine and now i only have a small headache. Thank you for the help!
Same here.. it was the reason why I did the video :D
How is each amplicon produced? How is the strand displacement accomplished, is it a function of the polymerase? Why is the loop formation necessary?
Although it seems simple, it's difficult to understand.. You made it really easy. Thank you for the explanation. :)
How both DNA denaturation and primer binding is occuring at same temperature?As both of them are opposite reaction?
the most clear explain ..
thanks
Excellent! Finally, I understood the loop structure formation. However, how does it makes the DNA duplication faster?
In PCR there are 3 cycles which combined take up some time to generate the daughter strands. In LAMP the synthesis continues constantly.. further: using 6 primers in case of LAMP, provides more contacts for polymerase recruitment!
Thank you for your video. i praise you to talk slowly and good pronunciation!! i'm about to subscribe your channel.
Heyy, I did LAMP today. The experimental setup was pretty straightforward. BUT, trying to understand the principles behind it, as concisely explained in your video is quite a challenge for me to wrap my head around it.
I'll have to do more reading for sure.. 😂
Thanks for the explanation!
Hello, I need your help.... ahaha
Can u explain me plz
Hi there. Is there any way you can turn on captions for this video? Thank you
Loved this explaination!!!
Very good!!!! Thank you!
perfect , thank you
I read that most DNA denatured at 75 degrees, does the DNA denatured at 60-65 degrees in LAMP or just some location? Can this become a limitation of LAMP?
Why do you only have the first product after the steps at 2:50. I thought the very first product is the one at 2:00 ?
Like and subscribed. Very good job 👏👍
Valeu!
The animation is very helpful. It will be even better if was shown at least one cycle after the annealing of external primers, indicated in 3:14. Unfortunately, I live in Brazil and my financial situation is not so good as I would like, but your animations deserve much more than $ 2.00. And thank you for answering my questions!
@@mariociencia12 Why do you donate, when your financial situation is not so good? I am extremely thankful, but I have to make that clear: I would not want anyone to donate if they have to keep an eye on their money!! Thanks anyways :)
*And you´re right - I spared myself the animation of the cycle
@@henrikslab I would like to donate more! You deserve! But the donation that I did will do not compromise me. Do not worry. I also did it as an example, hoping that other viewers do the same
@@mariociencia12 That´s very kind of you! But it helps me also so much when people like, subscribe and share the channel with friends!
great👍👍👍
perfect!
I don't know how the first strand invasion occurs
yey
capooooooooooooooooooooo
Thanks for the work that you put in. Hit me a massage if you like. I am a master in biochemistry and molecular biology maybe we can arrange something together.