Thanks foe sharing the input file formats. What does the error " assay# 1 of locusGenotypes. Two alleles seperated by any char in "/" required". How to overcome this error?
I think select all excel sheet except data cell and delete it. please do this both vertically and horizontal way and save file in tab deleminated format. Second one is see the specific cell may contain any special character. Third one is select appropriate data type is it bi-allelic or mono alleles. I think it will work.
@@sciexpos SCIEXPO above error occured when I did not change the genotype and marker to categeorological type. It got rectified after changing the type. Conpleted the analysis.
Very informative and helpful. sir, what to do missing data i have some few genotypes which is not amplified. can you please help me out with format. thanking you
If you have few genotype missing data i think you need to repeat amplification of those genotype. Other case use appropriate band data for those genotype. But before you to doing this think about what type of genotype population and band size amplify among the you get missing data in a marker or primers
I think you should have to watch another my video How to prepare molecular data set files? After watching these videos I think your query will be resolved. Best luck
I am unable to download the .net framework using the link given below. It is showing that download is no longer available. Please suggest me a solution
I've performed all the steps but "extra" (not in my list) samples appear in my tree, why does this happen? could you help me, please? Thanks in advance
@@sciexpos Thank you very much for the excellent manual for this program. I have the same problem, numbers appear in the tree along with genotype names. Instead of 20 genotypes, 40 are displayed on the tree.
@@512sector I solved it importing directly the 'frenquece based Nei's distance' matrix on Mega software. Mega don't recognize the matrix format obteined by genalex, so you should cut and paste the samples column at the end of matrix (on excel file).
@@dalilacrucitti7108 Thank you very much for your reply. How to transfer this matrix to the Mega program? I create a text file in the Mega program, transfer my matrix, but it does not want to work with this matrix.
@@512sector copy all(genalex matrix) on excel sheet. Add a new row in A1 Write the number of population in A1, C1, E1 Write the numbers of samples in B1,D1,F1 Row 2 empty Samples column at the end By genalex, click export data, choosing Mega, in the new window 'Mega export parameter" indicate #loci and #sample, data format TriMatrix, output to text file, click ok. Save As... Open Mega software, click Phylogeny, construct UPGMA tree, choose your txt file saved, but before recall it with ".meg" Open Click ok on error window Click replace: search for "," replace with "." and click OK File, save and collapse icon Return on phylogeny, construct tree, reopen file .meg Click ok on new window "analysis preferences" You have the TREE! :)
you have to install Microsoft .net framework 1.1(1.1.4..) Running manual installation gives you an error. follow cmd prompt for more ua-cam.com/video/-8X2rlPnx6I/v-deo.html use this link download .netframe works both redistributable and sp 1 service pack version(Sp1) save it in C drive and ensure the names of files are the same as in video especially sp1
Thanku for giving us detailed information.....its is very useful to every one......it is very clearly understandable in a simple language
Good very nice information
Very😄 good👌 video, Keep👨💻 It up👍...
Thank you
Expecting🙂 some more analysis💻 videos📹🎥
On the way 😊
Very nice information.. in simple language
Thank you dear 😊
Good work. Please send input file formats.
Thanks foe sharing the input file formats. What does the error " assay# 1 of locusGenotypes. Two alleles seperated by any char in "/" required". How to overcome this error?
I think select all excel sheet except data cell and delete it. please do this both vertically and horizontal way and save file in tab deleminated format. Second one is see the specific cell may contain any special character. Third one is select appropriate data type is it bi-allelic or mono alleles. I think it will work.
@@sciexpos SCIEXPO above error occured when I did not change the genotype and marker to categeorological type. It got rectified after changing the type.
Conpleted the analysis.
Could you help me, please? I have a triploid SSR data, how do I prepare my data? and how can I analyze it in powermarker software.
Very informative and helpful. sir, what to do missing data i have some few genotypes which is not amplified. can you please help me out with format. thanking you
If you have few genotype missing data i think you need to repeat amplification of those genotype. Other case use appropriate band data for those genotype. But before you to doing this think about what type of genotype population and band size amplify among the you get missing data in a marker or primers
@@sciexpos finally done, thank you
Could you help me, please? i have a triploïde SSR data, how can'i prepare my data? and how can'i analyse it in powermaeker software.
I have downloaded this power marker software but it is not opening ...plz keep the link of app below description thank you
Updated in the description, please share and Subscribe
Sir can you please make a video on GenAIEx. Thanking you sir in advance
Check this link for GenAlEx
ua-cam.com/video/2rFtDoHWUuw/v-deo.html
Brother is there any course that teaches all these softwares and how to use them ? How did u learn these.. Your videos are really good
Thanks
Try to read and understand manuals of each software. Where the author gives some examples file. All the best
Could you tell me how can I score in dataset in the case of the heterozygous genotype? For example, in same locus, It has both alleles 150 and 170
I think you should have to watch another my video How to prepare molecular data set files? After watching these videos I think your query will be resolved. Best luck
I got it. Thank you so much
I am unable to download the .net framework using the link given below. It is showing that download is no longer available. Please suggest me a solution
Software is in online you to search and install through cmd
@@sciexposWhat is cmd??
How to calculate polymorphic information content (PIC) value for SSRs marker data
All details have been explained in power Marker video that software calculate PIC for you
I've performed all the steps but "extra" (not in my list) samples appear in my tree, why does this happen? could you help me, please? Thanks in advance
I’m not getting what issue you have facing explain it. Or create new file and copy and paste as numerical format data, avoid spacing in names or ids.
@@sciexpos Thank you very much for the excellent manual for this program. I have the same problem, numbers appear in the tree along with genotype names. Instead of 20 genotypes, 40 are displayed on the tree.
@@512sector I solved it importing directly the 'frenquece based Nei's distance' matrix on Mega software.
Mega don't recognize the matrix format obteined by genalex, so you should cut and paste the samples column at the end of matrix (on excel file).
@@dalilacrucitti7108 Thank you very much for your reply. How to transfer this matrix to the Mega program? I create a text file in the Mega program, transfer my matrix, but it does not want to work with this matrix.
@@512sector copy all(genalex matrix) on excel sheet.
Add a new row in A1
Write the number of population in A1, C1, E1
Write the numbers of samples in B1,D1,F1
Row 2 empty
Samples column at the end
By genalex, click export data, choosing Mega, in the new window 'Mega export parameter" indicate #loci and #sample, data format TriMatrix, output to text file, click ok. Save As...
Open Mega software, click Phylogeny, construct UPGMA tree, choose your txt file saved, but before recall it with ".meg"
Open
Click ok on error window
Click replace: search for "," replace with "." and click OK
File, save and collapse icon
Return on phylogeny, construct tree, reopen file .meg
Click ok on new window "analysis preferences"
You have the TREE!
:)
I cannot download Powermarker software. kindly help me
It's old software, I don't know it's available or not. I think so u should go with Darwin software
@@sciexpos ok, Is it possible to calculate PIC value and resolving power of the SSR primer
How can i open a cluster tre file constructed in powermarker directly into mega software?
Yes the tree file will open in MEGA software do the modification in it.
which version of .net framework to be installed?
In a description link is already had given, So go through with
could you please tell me , how to prepare molecular data set file for power marker software ?
Yes there is video I have made how to prepare molecular data file formats
If i tried to open that file, a msg like "paint failed to open this file
I think it will not open in paint. You better yo try mega
I am facing problem in opening the .net framework. Its showing error. Please help
you have to install Microsoft .net framework 1.1(1.1.4..)
Running manual installation gives you an error. follow cmd prompt
for more ua-cam.com/video/-8X2rlPnx6I/v-deo.html use this link
download .netframe works both redistributable and sp 1 service pack version(Sp1)
save it in C drive and ensure the names of files are the same as in video especially sp1