The step on filtering 2.5ML of the serum, can someone explain that step to me? It was before coupling the affinity sepharose step. After the antibody and sepharose were coupled, did it become a serum? If so, was 2.5ML of the serum filtered and then coupled with sepharose? In @4:31 it didn't look like there was no sepharose in the dialysis. Were the video and instructions misaligned?
CNBr-activated agarose 4 Fast Flow is a well established, pre-activated chromatography medium for coupling of large amino-containing ligands. CNBr-activated agarose 4 Fast Flow is a widely used, successful and well-documented approach, with an easy, rapid and efficient coupling procedure .-Creative BioMart
The step on filtering 2.5ML of the serum, can someone explain that step to me? It was before coupling the affinity sepharose step.
After the antibody and sepharose were coupled, did it become a serum? If so, was 2.5ML of the serum filtered and then coupled with sepharose? In @4:31 it didn't look like there was no sepharose in the dialysis. Were the video and instructions misaligned?
CNBr-activated agarose 4 Fast Flow is a well established, pre-activated chromatography medium for coupling of large amino-containing ligands. CNBr-activated agarose 4 Fast Flow is a widely used, successful and well-documented approach, with an easy, rapid and efficient coupling procedure
.-Creative BioMart
2:10 時是用0.1M Tris還是1M Tris? 說明是0.1M但tube是1M?
You need to dialyse anitigen probably becouse the salt it contains could affect binding to the sepharose
why dialysis of antigen?
I think the dialysis should be done to the antibody. In the first step it is mentioned as antigen instead of antibody.
Are that spin column reusable?