Another way to remember your order of tubes is: 1. You= yellow 2. Bring= Light Blue 3. Really= Red-SST 4. Good= Green 5. Lollipops= Lavender 6. Girl= Gray I read the comments and understand I have missed some tubes, this is just a standard order of draw. Depending on the doctors office you will only use a SST and Lavender maybe a red, unless a doctor wants to check the patients INR which would be a light blue. That is based on my experience. Once you know the standard order of draw every other tube and blood culture will fall into place as well. Also this comment was from two years ago, in the state of TN they have changed some of our SSTs to gold. Good luck everyone and happy sticking :)
This video is AWESOME! it goes into all the details you need about Order of draw, what additives are in them, what department they go to, and what tests they are used for. All of this will help you to figure out the best answer to questions about specimen collection tubes when taking your NHA Phlebotomy exam!! I passed my exam today with a 424!!
I take my exam on nov 20 , if you have any advice or some study notes that you can pass me , i would appreciate it. Let me know and i could send you my email. and way to freaking go !!!! Im trying to get over 400 so im able to work in the NICU
Thank you very much! You are a gifted educator. I found your breakdown the best. I am taking a Phlebotomy course with only 6 days left and unfortunately we are out due to covid19. I decided to start rereading and studying everything we have covered and delighted that each time I go over it more sticks. Again thank you
I do not learn from the pop quiz at the end. I've been going over this for several weeks and I still cannot recall it. I took notes on everything you said and wrote. I'll review and maybe I'll remember...thank you for a clear explanation!
Mr Garza I'm from Trinidad and I'm going to start phlebotomy classes in January and I have already write down notes from your video to study in my spear time. Very good information. 👍👍👍👍👍
I'm yet to start my course because of location barrier. This was fun watching and love how detailed and easy explanations you gave. Thanks for the prep, it will really help me when i finally start my course .
thank you for this. my professor really isn't the best teacher, so this helped a lot. honestly there were colors like pink, gold and others and this clarified it much easier. I didn't know sst was also gold. lol
Gold really means “golden rod”, a darker yellow top we use for chemistry at my hospital. Also pink is special for blood bank that has a cross match label for the blood bank. We also use tiger tops (red and grey) for our BMP and CMP, and we have also used lavender for type and screens. so essentially FOLLOW YOUR INSTITUTIONS SOP and CLSI standards 😉 I’m a laboratory scientist and got to learn about all of this in great depth!
This video really helped a lot my instructor kind of started all over the place and this video helped me study and remember the tubes way easier! Thanks so much!
When the video starts it has great volume. But, when the study sessions start the volume is a little low and that's too bad. Other then that great work! I want to make a game for those who are having difficult times learning these tubes... some can learn the tubes but don't know the correct blood draws. So, it would be interesting to make a neat game of it. I would never have a failing study set.
loved this video and how simple but in depth it was at the same time, however im confused about the yellow tube. the order im being taught is first its blood cultures OR yellow tubes (SPS). im open to any explanations !
I have some clarification, Sir. If using the syringe method, does the red tube come first before the light blue? Since in the syringe, it doesn't use a tubing thing. Waiting for the response, thank you so much.
The only reason red would go before light blue is if you were drawing with a butterfly needle. There is air in the butterfly tubing that needs to be filled before using the light blue. With the syringe that is not the case when you transfer the blood. The order of draw remains the same.
SST is referred to as a "Tiger Top" but PST is usually a different color top tube. "Plasma Separator Tube" can come different colors depending on where you work.
In our program we only learned these colors. However, there’s other colors as well . So do we determine what order of draw we use by the additives in the tube ? Like for my skills final I got a gold/tigertop & a white pearl top ? What would be the order of draw ?
Here on our UA-cam channel we have other short video lessons that are from our 4 volume DVD collection. The whole collection is on sale for the month of June for $89 dollars on eBay or from our website. Here is the link for you. Thanks for your comments and I am glad they helped you. www.phlebotomysolutions.org/home-classes-dvds
I live in california and in school they mentioned to not use tiger tops because their not to be used anymore, so instead of a tiger top tube then what ttube can I use to replace the tiger top
genaro delucio I am not sure why they would say such a thing. The gray/black top tube is used by many including Labcorp, the largest independent lab company in the world. The majority of chemistry related test are collected in those tube or the green top tube with heparin.
Thank you so much for information of drawing blood tube.I am new here .I have to mauch experience of laboratory work but from my country.I think this will help me to find right place for work.
I've seen several different color charts in several different places. I know the order of the draw is more based on chemicals in the tubes, but do the chemicals in the tubes change what color tubes they go in from venue to venue?
In blood draws, certain samples may need to be put on ice to preserve their integrity. These samples typically include tests that require cold storage, such as lactate, ammonia, and certain enzymes. Placing the blood samples on ice helps maintain their stability and prevents any degradation or changes in the composition of the sample.
There appear to be some errors in your video. The polymer "gel" is NOT responsible for accelerating clotting. It actually just serves as a barrier device ("separator") between the serum and the cells upon centrifugation; this barrier effect inhibits glycolysis. The additive which assists in clotting is a silica spray - it is not an accelerator, but an activator of clotting (such that it helps the blood form a complete clot). A clot accelerator would be thrombin (orange top tube = RST). Further, the PST tubes do not contain fibrinogen. If this were the case, the PST tube would clot and you wouldn't yield plasma. A PST tube is simply a tube with polymer gel and an anticoagulant (usually heparin). If you are referring to the fact that PLASMA contains fibrinogen and SERUM does not, then I think you may want to be more clear on that. Also, I am not sure why you mention royal blue coming after the ACD tube. Royal blue tubes - at least the ones that I have worked with - either contain EDTA or Silica spray. Where the royal blue fits into order of draw would depend on which tube you have - EDTA or silica.
Thank you for your comment. Here is my response. 1. Compared to SST, RST is considered an accelerator or faster (2.49 min vs. 19.47 min, respectively; P < 0.001). There was a statistically significant difference between the RST and SST II tubes for glucose, calcium, and LD (P < 0.001). Differences for glucose and LD were also clinically significant. Analyte stability studies showed that all analytes were stable for 24 h at 4 °C. www.ncbi.nlm.nih.gov/pmc/articles/PMC4210257/ SST is a clot activator and substance that enhances coagulation and clots within 15 to 30 minutes as compared to other test tubes. It can be considered an accelerator. 2. There is only one kind of PST tube. It has a mint green stopper. This tube is only used on patients who are in the hospital. This tube contains lithium heparin to keep the blood from clotting and has a gel plasma separator. This tube is used to obtain whole blood, plasma. To ensure that the blood does not clot, the tube must be inverted eight times following blood collection. This tube is used for therapeutic drug monitoring and general chemistries. Because the PST™ tube contains lithium, it cannot be used to test a patient’s lithium level. (Phlebotomy Training Group) 3. I was referring to the fact that PLASMA contains fibrinogen and SERUM does not. I made that distinction in the video. 4. Royal blue is followed in the order of draw set by CLSI Standards.
amy castellano you are right even I’m a student in the medical field I still noticed about the order the tubes and when he explains the green tube and grey tube. He should take his time next even though he’s in the medical field mistakes like this is a bit risky and people watching and learning not knowing there’s mistakes in it.
Alondra Medina Fibrinogen is in the blood and when blood is put in the PST tube then the lab can test it with the blood. But in a SST tube they can not test it.
Alondra Medina Fibrinogen is in the blood and when blood is put in the PST tube then the lab can test it with the blood. But in a SST tube they can not test it. Plasma contains fibrinogen with a PST tube but with a SST tube it doesn’t.
@@yashidolmamoktan4674 Typically a lavender top EDTA tube is used to collect for the test. So the order will be after green and after a CBC if ordered with it. But always consult the lab first.
Thank You!!! Wish you can help me pass my Exam though!! its so long and boring!! I love the ending!! wish you can do more of those things!! like what to draw with what?
my teacher said that red tube is for CMP and BMP. I just want to confirm that it is actually the green tube? So confused. LabCorp website says you can use both. Ughhhh. Which do we need to know for the national exam?
Hello, thank you for the question. If you are in a hospital setting then you will be using a green top tube for all chemistry tests which includes a BMP and CMP. For transporting blood to a lab like Labcorp does then they will use a SST tube to collect the blood and process it for transporting. I know this because I was a Labcorp supervisor for 2 years. You may use a red top non-additive tube but the blood will be transferred to another tube with Heparin for testing. For the test you will need to know the standard order of draw that is listed in this video and set by CLSI. I hope this helps you. Thank you.
@@PhlebotomySolutions Thank you for the guidance and clarification. So I will memorize the tests and colored tubes listed in this video to prepare for the exam. I really appreciate your help and for getting back to me so quickly! Thank you so much!
I am being taught that the BMP and CMP tests are done in the Gold/Tiger top tube. You mentioned that it is done on the Dark Green Tube. I am also being taught that Electrolytes and HCG are done in Light Green Tubes and Ammonia test are done on Dark Green tubes. Are these recent changes?
DW Ethiopia Hello, your question is based on what phlebotomy job you have and where. You can run a BMP and CMP on either a SST tube or in a Green Lithium Heparin tube for faster test results. The lab machines being used also determine which tube to use too. For Ammonia you will use lithium Heparin Green tube and put immediately in ice. All chemistry test can be done in a Gold/tiger top tube or a Green lithium Heparin tube. Make sure the Green tube is NOT light Green sodium Heparin. Every hospital and lab uses either tube for chemistry tests based on their machine running the tests. Hope this helps.
@@PhlebotomySolutions I don't have a job in Phlebotomy yet. This is information I am learning at a Phlebotomy school. What I am being taught in my class contradicts what you are saying in your video. For ammonia, I am taught that it is done in a Dark Green tube, which has the sodium heparin additive. I am taught that lithium heparin is in the light green/mint tube. This is the opposite of what you are saying.
DW Ethiopia Sorry, I need to mention that tubes can change based on color top tubes. The additives is what matters more than what the top color is on the tube. Dark green would be sodium Heparin and light green is lithium Heparin. Always go by what is labeled in the tube for testing. Every lab and hospital can change based on what machine they use for processing the tubes.
DW Ethiopia Ammonia is collected in light Green lithium additive tube. But certain hospitals can collect the blood in a different tube and processes it with the correct testing device used. Labs can collect ammonia test in a lavender tube and then frozen for send out too. That is what Labcorp does. Always check with your hospital or lab for their processing procedures.
Michael JonesDougherty. To my knowledge the pink tube is still being used for blood bank. It contains the same additive as the lavender, EDTA, and is still being used by hospitals.
The hospitals and labs should only be cleaning the site with soap and water. NO antiseptic should be used. All of them contain some kind of chemical or alcohol that can contaminate the test. This is also recommended by CLSI.
@@miriamalvidrez1409 That would depend on the lab you work for. Every lab has different tube top colors with different additives. Consult your lab for the tubes they use with their additives for the order of draw.
@@miriamalvidrez1409 The color top usually tells you what tests need to done. The additives can be the same but the tops can be different based on where they need to go be tested.
Tiffany Bishop Yes, this information is directly from CLSI standard order of draw used in every lab. Color of tops may change but the additive base order is the same.
can you give me information about Royal blue , some tubes contain NH and some contain EDTA and some have clot activator , what is the diffrints between all of them ?
Khalaf Majed Every tube color is based on the additive and test that will be run. If you look up the additive online you will see the tests that will be performed based on the additives. The color usually tells you the department that the test will be performed.
Khalaf Majed Again, each Royal blue tube can contain different of the same additive. They Contain either sodium heparin or sodium EDTA anticoagulants, or no anticoagulant. And Are used for trace element, toxicology, and nutritional studies. You need to consult lab on tests. Royal Blue acid washed tubes are available in No Additive clot tubes for serum collection as well as EDTA and Sodium Heparin additives for plasma collections. Please take care to inspect the tube label to identify the additive type. Royal Blue topped tubes are special acid washed tubes for the collection of trace elements or heavy metals and cellular immunology studies.
Am not sure when this video was maid. But am in phlebotomy and the order that am learning is a bit different. (Gold, red/Grey Sotted tube) goes before red. And we have a royal/ Navy blue that is the last one after grey. I'm guessing machines in clinics and hospitals change so also the order of tubes can be or must be changed. Love your video great information 👍🏼👍🏼
Karina Venegas The order of draw does not change based on CLSI standards. The order is based on the additives and not really the tube colors. The Gold or culture tubes or bottles are always first. The red/gray or SST tube can go before the red non-additive tube. The Royal blue is after the gray tube. It’s the light blue tube with Sodium Citrate that is used after the gold or cultures.
I was liking the video until I heard you say you use a green sodium heparin tube to run a CMP. That would interfere with the sodium level on the CMP and now I'm questioning everything being said. You need to use the mint green lithium Heparin tube to run a CMP.
The green top tube with sodium heparin additive does not contain sodium ions that could interfere with the sodium level measurement in a CMP. The sodium heparin additive is used to prevent blood clotting by inhibiting the action of thrombin, but it does not affect the measurement of sodium or other electrolytes in the blood. Therefore, it is safe to use the green sodium heparin tube for running a CMP.
@@teresitabrown678 The yellow is part of the sterile or blood culture category and is always drawn first in the order. The video does not show blue or purple before those tubes.
Actually vacutainer is a brand name but not the tube's. These are, as you know, called ETS systems or Evacuated Tubes (Vacuum Blood Tubes). There are many brands manufacturing these.
Louis Felix Hello, most of these videos on my channel are for education to help prepare you for the test. I also have 4 DVDs at my website PhlebotomySolutions.org that are made to help on tests too. Available also at EBay too and Amazon.
I really appreciate the way to teach.I just like to know whether the order of draw and all other steps involved in venepuncture vary in different places or not. I live in UK.
Great question. The order of draw is based on the additives in the tube which is universal. The tube top color may change in your area so check with your facility. The additives in the order of draw will not change.
Devin Jackson If both are SST, then yes. Red tops can be a non additive which has a different place in the order of draw. The additives will tell you which goes first to last in the order. It’s all based on the additives in the tubes.
I remember it as You Like Really Good Looking Guys (yellow, light blue, red, green, lavender, gray)
Thank you very helpful
That was helpful
I like that one😁
I like this analogy. I start class next month. Thanks for sharing 👍
that's a good one Kamai!! way to go!! but I don't think I would need this as a cardiologist I will have my wonderful nurses do that haha
Another way to remember your order of tubes is:
1. You= yellow
2. Bring= Light Blue
3. Really= Red-SST
4. Good= Green
5. Lollipops= Lavender
6. Girl= Gray
I read the comments and understand I have missed some tubes, this is just a standard order of draw. Depending on the doctors office you will only use a SST and Lavender maybe a red, unless a doctor wants to check the patients INR which would be a light blue. That is based on my experience. Once you know the standard order of draw every other tube and blood culture will fall into place as well. Also this comment was from two years ago, in the state of TN they have changed some of our SSTs to gold. Good luck everyone and happy sticking :)
I appreciate this so much!!!!!!
Jamaican gyal yes of course! Good luck 💕
Thanks so much
Gopaul Jairam you’re welcome :)
Adrienne Walton thank you.
I like this type of instruction. No wasted time with worthless words.
Thank you.
This video is AWESOME! it goes into all the details you need about Order of draw, what additives are in them, what department they go to, and what tests they are used for. All of this will help you to figure out the best answer to questions about specimen collection tubes when taking your NHA Phlebotomy exam!! I passed my exam today with a 424!!
Adrinah Thank you for the comment. And congratulations on passing! 🎉
I take my exam on nov 20 , if you have any advice or some study notes that you can pass me , i would appreciate it. Let me know and i could send you my email. and way to freaking go !!!! Im trying to get over 400 so im able to work in the NICU
How many questions are on the test and how much do yo need to pass?
Thank you very much! You are a gifted educator. I found your breakdown the best. I am taking a Phlebotomy course with only 6 days left and unfortunately we are out due to covid19. I decided to start rereading and studying everything we have covered and delighted that each time I go over it more sticks. Again thank you
Shari Cook Thank you. I appreciate your comment. I’m glad the videos are helping.
1:56 that mnemonic is so brilliant! I’ll be taking my NCCT in phlebotomy next Wednesday so wish me luck!
I took the NCPT test last week I also had done an internship for 2weeks in labcorp
I do not learn from the pop quiz at the end. I've been going over this for several weeks and I still cannot recall it. I took notes on everything you said and wrote. I'll review and maybe I'll remember...thank you for a clear explanation!
Thank you So much for this very helpful information I passed my PCT exam!!!!!!
Lakesha Hunt That is great. Congratulations!!🎈🎉
Im just now seeing this post, but I start class next week. Im so nervous.
I’m taking mine tomorrow. if you see this any tips?
@@NaturalBeauty-qe7mk How dis your class go? Did you pass? I am starting next week.
@@sotoalexandra57 hey.. Yes I did pass the class. Im officially an CCMA, CPT. 😁
Mr Garza I'm from Trinidad and I'm going to start phlebotomy classes in January and I have already write down notes from your video to study in my spear time. Very good information. 👍👍👍👍👍
Carla Modeste Thank you. Keep watching the videos.
I will be starting my phlebotomy course in 2 weeks and this will help a lot! Thank you!
Omg! That was so helpful! And that memory jogger is a life saver!
brandeewyne J Thank you, I’m glad it helped.
I'm yet to start my course because of location barrier.
This was fun watching and love how detailed and easy explanations you gave. Thanks for the prep, it will really help me when i finally start my course .
Thank you for watching.
taking my cpt tomorrow. this has helped alot thank you so much !
Bloody Pup You are very welcome.
thank you for this. my professor really isn't the best teacher, so this helped a lot. honestly there were colors like pink, gold and others and this clarified it much easier. I didn't know sst was also gold. lol
I am glad to help. We also have all our videos and more on 4 DVDs available on Amazon and EBay.
Got to check it out. What dvds would I be looming for?
michael rubinstein Phlebotomy Solutions DVD in Amazon or EBay. Also check our website PhlebotomySolutions.org
Lol
Gold really means “golden rod”, a darker yellow top we use for chemistry at my hospital. Also pink is special for blood bank that has a cross match label for the blood bank. We also use tiger tops (red and grey) for our BMP and CMP, and we have also used lavender for type and screens. so essentially FOLLOW YOUR INSTITUTIONS SOP and CLSI standards 😉 I’m a laboratory scientist and got to learn about all of this in great depth!
The best of the best, thank you, please keep teaching. You're made for this
Jonathan Rodriguez Thank you for that comment.
This video really helped a lot my instructor kind of started all over the place and this video helped me study and remember the tubes way easier! Thanks so much!
I’m glad the video helped you. Thank you for watching.
I like your method of instruction straight forward and no nonsense verbage.
Thank you.
When the video starts it has great volume. But, when the study sessions start the volume is a little low and that's too bad. Other then that great work! I want to make a game for those who are having difficult times learning these tubes... some can learn the tubes but don't know the correct blood draws. So, it would be interesting to make a neat game of it. I would never have a failing study set.
Thanks for the comment. The audio was checked and is working fine on the video. You might want to check you computer levels.
I seriously thought something went wrong with my phone
your video volume is too low, I can hardly hear you, please consider when making videos turning up the volume or talking louder
This is extremely helpful. This was the only thing that confused me in class
Thank you and I am glad it helped.
Perfect video. It's very easy to remember. Thank you so much.
@@skylarsmith6566 Thank you for watching.
nice info , just a reminder . in gray top tube , the Na- fluoride additive is an antiglycolytic not antiglycocytic.. thanks
COSMICDUST Thanks, we misspelled the word.
that jogger is so confusing . my teacher taught us (Y)oung (B)oys (R) (S)till (G)etting (L)ow (G)rades Yellow Light Blue Red Green Lavender Gray
niece511 The jogger was created by CLSI. It works for some. If your jogger works for you then that is great.
Thanks for the video. It helped me to remember the tubes with mnemonic. I passed NHA exam at first time. ❤❤❤❤❤
@@gangagurung8807 Thank you for watching. 🙏
@@gangagurung8807 Congratulations! Thank you for watching!
you welcome.
You
Live
Right
Growing
Loving
God
I love this one!
this actually was so much better to remember than the poem they tried to use in my class lol !
Thank You !!
Thank you for watching.
loved this video and how simple but in depth it was at the same time, however im confused about the yellow tube. the order im being taught is first its blood cultures OR yellow tubes (SPS). im open to any explanations !
Hello. At the beginning of the video it states yellow-sterile and or blood cultures. Both are equally used before each other.
@@PhlebotomySolutions thank you so much ! you've helped me so much!!
@@roobee9175 You are welcome. Thank you for watching.
Starting phlebotomy course on the 16th, very excited!
I saved this video, the explanation is awesome 👌
Thank you for watching.
thanks for sharing and helping i am a CPT but there is always more space to learn
Thank you for your VDO series. They have been very helpful.
Best video straight to the point
GREAT JOB, THIS WAS A GREAT LEARNING VIDEO
rlalko l
Thank you for your comment.
Thank you. Still relevant video 3 years later.
Thank you.
Love this totally helped my studying thank you!
Ashlee Carrero Thank you. I’m glad they helped.
This is great! I've been struggling with this for a bit. Cleared a lot of things up for me
Sara Mizzi I'm glad it helped you. Thank you for watching.
thank you for this video i have a test in 4 days and did know how i was going to remember this . Very helpful
Krystynna Harris You are welcome. I’m glad it helped.
Is this the latest order of draw? I have seen others that are a little different and want to make sure I am getting the correct information.
Jordan Amspacher Yes, this is directly from CLSI. Up to date
Thank you for the information Mr Garza.
when should you draw the dark blue toxicology tube? it was not actually mentioned in terms of order of draw.
You draw the dark blue after the gray tube at the end. I believe I mentioned it in the video.
i like the pattern you have shared to me...and i will practice it with my work.it helps me and supports my additional knowledge..thank you..
flordeliza pelino You are welcome and I’m glad it helped.
Lovely video. Your students should feel lucky
Bruna N Thank you for your comment.
I saw one for and it goes
the sky-light blue
the sun-red/gold/red tiger
the grass-green
the flowers- lavender and pink
the pavement-grey
I have some clarification, Sir. If using the syringe method, does the red tube come first before the light blue? Since in the syringe, it doesn't use a tubing thing. Waiting for the response, thank you so much.
The only reason red would go before light blue is if you were drawing with a butterfly needle. There is air in the butterfly tubing that needs to be filled before using the light blue.
With the syringe that is not the case when you transfer the blood. The order of draw remains the same.
Thank you for doing this video my name is Dolores Michel and med student. I enjoy the video 😄
Thank you for watching!
1. Yellow
2. Light blue
3. Red
4. Green
5. Lavender
6. Gray
Don't forget SST and PST too.
Jeff KoCaine easy
SST and PST are called "Tiger top".- and they are interchangeable with red in terms of order of draw.
SST is referred to as a "Tiger Top" but PST is usually a different color top tube. "Plasma Separator Tube" can come different colors depending on where you work.
In our program we only learned these colors. However, there’s other colors as well . So do we determine what order of draw we use by the additives in the tube ? Like for my skills final I got a gold/tigertop & a white pearl top ? What would be the order of draw ?
Great question. The order of draw is based on the additives in the tube. Check the additive in the Pearl top and go from there.
thank you !!! this video helped me to understand more about why and how is the function ets colors thanks
norma ordunez I am glad the video helped. Please check out the others too. Contact me if you have questions.
Phlebotomy Solutions hi thanks for answer my comment , where I can see your others videos , can you send me the web or the media please !!! gracias
Here on our UA-cam channel we have other short video lessons that are from our 4 volume DVD collection. The whole collection is on sale for the month of June for $89 dollars on eBay or from our website. Here is the link for you. Thanks for your comments and I am glad they helped you.
www.phlebotomysolutions.org/home-classes-dvds
Fantastic you explain in detailed thanks.
Thank you and thank you for watching.
Amazing!! Thank you so much; it helped me a lot just to clear things out. Well done.
Nishi N I’m glad it helped. Thank you for posting.
the best explanation
Thank you for watching
I’ve once seen a special tube with brown background and grey speckles, what is that used to test actually?
@@zhining911 what is the additive listed on the tube?
Thanks for sharing. Why didn't you add the pink top tube in the memory jogger?
Amanda Darling The CLSI standard order of draw is what is required. The pink is EDTA for blood bank and is not part of the “rainbow” draw.
I live in california and in school they mentioned to not use tiger tops because their not to be used anymore, so instead of a tiger top tube then what ttube can I use to replace the tiger top
genaro delucio I am not sure why they would say such a thing. The gray/black top tube is used by many including Labcorp, the largest independent lab company in the world. The majority of chemistry related test are collected in those tube or the green top tube with heparin.
Thanks for the video. I would like to know if I can share this video with my employees? I wanted to make sure I'm not violating any copyright law.
Thank you so much for information of drawing blood tube.I am new here .I have to mauch experience of laboratory work but from my country.I think this will help me to find right place for work.
Priti Soni I’m glad you enjoyed the video.
I've seen several different color charts in several different places. I know the order of the draw is more based on chemicals in the tubes, but do the chemicals in the tubes change what color tubes they go in from venue to venue?
The color top changes based on department. The additive can be the same in two tubes but go to different departments like lavender and pink tops.
Which ones are on iice? Lactate and calcium citrate? I'm not sure.
In blood draws, certain samples may need to be put on ice to preserve their integrity. These samples typically include tests that require cold storage, such as lactate, ammonia, and certain enzymes. Placing the blood samples on ice helps maintain their stability and prevents any degradation or changes in the composition of the sample.
This video was very helpful
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There appear to be some errors in your video. The polymer "gel" is NOT responsible for accelerating clotting. It actually just serves as a barrier device ("separator") between the serum and the cells upon centrifugation; this barrier effect inhibits glycolysis. The additive which assists in clotting is a silica spray - it is not an accelerator, but an activator of clotting (such that it helps the blood form a complete clot). A clot accelerator would be thrombin (orange top tube = RST). Further, the PST tubes do not contain fibrinogen. If this were the case, the PST tube would clot and you wouldn't yield plasma. A PST tube is simply a tube with polymer gel and an anticoagulant (usually heparin). If you are referring to the fact that PLASMA contains fibrinogen and SERUM does not, then I think you may want to be more clear on that. Also, I am not sure why you mention royal blue coming after the ACD tube. Royal blue tubes - at least the ones that I have worked with - either contain EDTA or Silica spray. Where the royal blue fits into order of draw would depend on which tube you have - EDTA or silica.
Thank you for your comment. Here is my response.
1. Compared to SST, RST is considered an accelerator or faster (2.49 min vs. 19.47 min, respectively; P < 0.001). There was a statistically significant difference between the RST and SST II tubes for glucose, calcium, and LD (P < 0.001). Differences for glucose and LD were also clinically significant. Analyte stability studies showed that all analytes were stable for 24 h at 4 °C. www.ncbi.nlm.nih.gov/pmc/articles/PMC4210257/
SST is a clot activator and substance that enhances coagulation and clots within 15 to 30 minutes as compared to other test tubes. It can be considered an accelerator.
2. There is only one kind of PST tube. It has a mint green stopper. This tube is only used on patients who are in the hospital. This tube contains lithium heparin to keep the blood from clotting and has a gel plasma separator. This tube is used to obtain whole blood, plasma. To ensure that the blood does not clot, the tube must be inverted eight times following blood collection. This tube is used for therapeutic drug monitoring and general chemistries. Because the PST™ tube contains lithium, it cannot be used to test a patient’s lithium level. (Phlebotomy Training Group)
3. I was referring to the fact that PLASMA contains fibrinogen and SERUM does not. I made that distinction in the video.
4. Royal blue is followed in the order of draw set by CLSI Standards.
amy castellano you are right even I’m a student in the medical field I still noticed about the order the tubes and when he explains the green tube and grey tube. He should take his time next even though he’s in the medical field mistakes like this is a bit risky and people watching and learning not knowing there’s mistakes in it.
PLASMA contains fibrinogen and SERUM does not. is this in the blood or in the tube as an additive? I'm confused please help! @@PhlebotomySolutions
Alondra Medina Fibrinogen is in the blood and when blood is put in the PST tube then the lab can test it with the blood. But in a SST tube they can not test it.
Alondra Medina Fibrinogen is in the blood and when blood is put in the PST tube then the lab can test it with the blood. But in a SST tube they can not test it.
Plasma contains fibrinogen with a PST tube but with a SST tube it doesn’t.
My phleb class uses your videos lol
Wow! Thank you for letting me know. I’m glad my videos are being used to help others.
Thank you very much for a well explained and clear presentation.
Thank you for the comment.
Lifesaver. Thank you.
Silvan Aban Glad it helped.
When blood should be drawn in black vial used for ESR ?
When it is ordered by the doctor.
@@PhlebotomySolutions i mean in order of collection time after which tube should be collected?
@@yashidolmamoktan4674 Typically a lavender top EDTA tube is used to collect for the test. So the order will be after green and after a CBC if ordered with it. But always consult the lab first.
@@PhlebotomySolutions thank you:)
Thank You!!! Wish you can help me pass my Exam though!! its so long and boring!! I love the ending!! wish you can do more of those things!! like what to draw with what?
We are working on more videos.
Phlebotomy Solutions Thank you so much ☺️. I’m learning so much from your videos and I bought your book from Amazon 10/10!
@@Errsvydiibc Great! I’m happy my videos are helping. Don’t forget to leave a review about the book at Amazon. That really helps.
What about royal blue with a stripe?
You would need to check the additive first.
Love this vedic Great job your a wonderful teacher thank you so much for your help.
It's more like " you like really strong powerful good looking guy* 😊
my teacher said that red tube is for CMP and BMP. I just want to confirm that it is actually the green tube? So confused. LabCorp website says you can use both. Ughhhh. Which do we need to know for the national exam?
Hello, thank you for the question. If you are in a hospital setting then you will be using a green top tube for all chemistry tests which includes a BMP and CMP.
For transporting blood to a lab like Labcorp does then they will use a SST tube to collect the blood and process it for transporting. I know this because I was a Labcorp supervisor for 2 years.
You may use a red top non-additive tube but the blood will be transferred to another tube with Heparin for testing.
For the test you will need to know the standard order of draw that is listed in this video and set by CLSI.
I hope this helps you. Thank you.
@@PhlebotomySolutions Thank you for the guidance and clarification. So I will memorize the tests and colored tubes listed in this video to prepare for the exam. I really appreciate your help and for getting back to me so quickly! Thank you so much!
another also to remenber the order of draw is
you- yellow
bring- blue
romans- red
love- lavander or purple
and
gentle- gray
I am being taught that the BMP and CMP tests are done in the Gold/Tiger top tube. You mentioned that it is done on the Dark Green Tube. I am also being taught that Electrolytes and HCG are done in Light Green Tubes and Ammonia test are done on Dark Green tubes. Are these recent changes?
DW Ethiopia Hello, your question is based on what phlebotomy job you have and where. You can run a BMP and CMP on either a SST tube or in a Green Lithium Heparin tube for faster test results. The lab machines being used also determine which tube to use too.
For Ammonia you will use lithium Heparin Green tube and put immediately in ice.
All chemistry test can be done in a Gold/tiger top tube or a Green lithium Heparin tube. Make sure the Green tube is NOT light Green sodium Heparin.
Every hospital and lab uses either tube for chemistry tests based on their machine running the tests.
Hope this helps.
@@PhlebotomySolutions I don't have a job in Phlebotomy yet. This is information I am learning at a Phlebotomy school. What I am being taught in my class contradicts what you are saying in your video. For ammonia, I am taught that it is done in a Dark Green tube, which has the sodium heparin additive. I am taught that lithium heparin is in the light green/mint tube. This is the opposite of what you are saying.
DW Ethiopia Sorry, I need to mention that tubes can change based on color top tubes. The additives is what matters more than what the top color is on the tube. Dark green would be sodium Heparin and light green is lithium Heparin. Always go by what is labeled in the tube for testing.
Every lab and hospital can change based on what machine they use for processing the tubes.
@@PhlebotomySolutions Ok thank you very much! Appreciate the video content. Happy Holidays!
DW Ethiopia Ammonia is collected in light Green lithium additive tube. But certain hospitals can collect the blood in a different tube and processes it with the correct testing device used.
Labs can collect ammonia test in a lavender tube and then frozen for send out too. That is what Labcorp does.
Always check with your hospital or lab for their processing procedures.
In school they say there takeing out the pink tube . There no longer useing it.?
Michael JonesDougherty. To my knowledge the pink tube is still being used for blood bank. It contains the same additive as the lavender, EDTA, and is still being used by hospitals.
Great presentation, but your background colors should match your stopper tubes colors. I remember red because of thst, just saying.
Which antiseptic is recommended for blood-alcohol testing? I keep getting conflicting info and I trust you.
The hospitals and labs should only be cleaning the site with soap and water. NO antiseptic should be used. All of them contain some kind of chemical or alcohol that can contaminate the test.
This is also recommended by CLSI.
Thank you!
I really appreciate your help!
Where does Pink, Tan, Royal Blue, White, Black and Orange Top Tubes fit in this?
Those are usually special order draws and are last based on the additives.
@@PhlebotomySolutions if all the 12 tubes were filled with their respective additives, where would they go in the list?
@@miriamalvidrez1409 That would depend on the lab you work for. Every lab has different tube top colors with different additives. Consult your lab for the tubes they use with their additives for the order of draw.
@@PhlebotomySolutions I noticed that! Ughhh... why are there different color tubes and different additives? 😑 Why can't they be universal?
@@miriamalvidrez1409 The color top usually tells you what tests need to done. The additives can be the same but the tops can be different based on where they need to go be tested.
Is this information up to date?
Is this information for any state?
Tiffany Bishop Yes, this information is directly from CLSI standard order of draw used in every lab. Color of tops may change but the additive base order is the same.
In my book it says the gold is the SST tube. How come they both are the same for the red/gray?
Thank you so much for the video.
can you give me information about Royal blue , some tubes contain NH and some contain EDTA and some have clot activator , what is the diffrints between all of them ?
Khalaf Majed Every tube color is based on the additive and test that will be run. If you look up the additive online you will see the tests that will be performed based on the additives. The color usually tells you the department that the test will be performed.
Phlebotomy Solutions thanks for your , but is the royal blue tube with K2-EDTA can do the tests for the additive in lavender tube?
Khalaf Majed Again, each Royal blue tube can contain different of the same additive.
They Contain either sodium heparin or sodium EDTA anticoagulants, or no anticoagulant.
And Are used for trace element, toxicology, and nutritional studies.
You need to consult lab on tests.
Royal Blue acid washed tubes are available in No Additive clot tubes for serum collection as well as EDTA and Sodium Heparin additives for plasma collections. Please take care to inspect the tube label to identify the additive type. Royal Blue topped tubes are special acid washed tubes for the collection of trace elements or heavy metals and cellular immunology studies.
Phlebotomy Solutions great I understand now , but i need more information about acid washed tubes ?
@@khalaf7299 Typically it is referring to ACD or Acid CItrate Dextrose for blood bank studies.
Ohhh this is very helpful!
Am not sure when this video was maid. But am in phlebotomy and the order that am learning is a bit different. (Gold, red/Grey Sotted tube) goes before red. And we have a royal/ Navy blue that is the last one after grey. I'm guessing machines in clinics and hospitals change so also the order of tubes can be or must be changed. Love your video great information 👍🏼👍🏼
Karina Venegas The order of draw does not change based on CLSI standards. The order is based on the additives and not really the tube colors. The Gold or culture tubes or bottles are always first. The red/gray or SST tube can go before the red non-additive tube. The Royal blue is after the gray tube. It’s the light blue tube with Sodium Citrate that is used after the gold or cultures.
thank you 🙏 you are just great teacher !
Alondra Medina Thank you for the support.
Your background color should match the tube you are talking about.
I was liking the video until I heard you say you use a green sodium heparin tube to run a CMP. That would interfere with the sodium level on the CMP and now I'm questioning everything being said. You need to use the mint green lithium Heparin tube to run a CMP.
The green top tube with sodium heparin additive does not contain sodium ions that could interfere with the sodium level measurement in a CMP. The sodium heparin additive is used to prevent blood clotting by inhibiting the action of thrombin, but it does not affect the measurement of sodium or other electrolytes in the blood. Therefore, it is safe to use the green sodium heparin tube for running a CMP.
I thought yellow was supposed to be first......you had on one of the slides that purple or blue top must be drawn first.
@@teresitabrown678 The yellow is part of the sterile or blood culture category and is always drawn first in the order. The video does not show blue or purple before those tubes.
Love this vídeo! Very good explanation
Olivia A-Van Boekhout Thank you.
Phlebotomy Solutions I’m studying phlebotomy . If you have more videos let me know pls
Actually vacutainer is a brand name but not the tube's. These are, as you know, called ETS systems or Evacuated Tubes (Vacuum Blood Tubes). There are many brands manufacturing these.
This is great. Can you do more with what is need for the test
Louis Felix Hello, most of these videos on my channel are for education to help prepare you for the test.
I also have 4 DVDs at my website PhlebotomySolutions.org that are made to help on tests too.
Available also at EBay too and Amazon.
Louis Felix yes what he
said👆🏻
Snow White I offer a phlebotomy exam study flash drive for students too.
Go to www.phlebotomysolutions.org
great video! now more easy for me to remember the order of draw.
Cherry L Thank you. Glad it helped.
Cherry L nice
Is the Gold SST and Red Tiger top SST the same?
Lois Tindal Hello, they are the same. The tops are different mainly to show where the tube is going and what is being tested.
Hello are you ALLOWED to Centrifuge Whole Blood or no ???
zuley palma Yes, but only for certain tests like glucose and certain chemistry 🧪 tests .
Great videos, you are awesome.
Iam so struggle with which one iam gonna used and when.
Me to!!
I'm confused some people start order of draw with yellow some with blue so which is the correct one
That’s what I don’t understand
Thank you for well explained the order of draw.
Thank you for the comment and watching.
I really appreciate the way to teach.I just like to know whether the order of draw and all other steps involved in venepuncture vary in different places or not. I live in UK.
Great question. The order of draw is based on the additives in the tube which is universal. The tube top color may change in your area so check with your facility. The additives in the order of draw will not change.
@@PhlebotomySolutions Thank you so much for the prompt reply..
@@neethyjinesh You are welcome.
very nice👍
Thank you for watching.
Thank you so much for this informative video!
AR You’re very welcome.
Want to know which tube go to cystology and microbiology also serology/immunoloy
Each hospital lab is different. Blood cultures go to micro while type and screens or cross matches go to blood bank or serology.
Thank you please more videos so amazing
Ninja Fan
Thank you. I’m glad you enjoyed. We will continue to make more..
Great video. God bless.
safiya kamila thank you
Tiger tops and Red tops are the same in the order of draw?
Devin Jackson If both are SST, then yes. Red tops can be a non additive which has a different place in the order of draw. The additives will tell you which goes first to last in the order.
It’s all based on the additives in the tubes.
Well explained !
Thanks so much.
hi is this suitable for the uk? tia :)
oroboras Yes, it’s suitable everywhere.