Simple and classy way of explaining..... really you explained not just protocol but the concept behind it..thanks for the efforts......best wishes for upcoming videos.
Sorry to saying it im not flirting with you but seriously i cant concentrate on concept while you studying bcoz of ur beauty ....sorry n thank you for giving us valuable information
Ma'am thank you. App bahut achche se samjhati h aur samjh me bhi asani se aa Jata h. Mai Aapke Sare video dekhta hu. Ma'am Corona virus pr bhi video banaiye.
thanks mam for such a nice video.. you explain in very short and sweet way always. please make some videos on antibiotic resistance,and its types of efflux pumps. thanks again.. keep up..
Mam do min to apko dekhne mein hi chale jate hain phir dhyan aata hain padhna bhi hain . Just joke yes i understand your concept easily Keep uploading ..
BaaYo Dear Madam I had applied in NIPER for VU-VIS spectroscopy, GC and HPLC chromatography Practical training short courses but there is no replay from NIPER. Can u please help me to recommend other Institutions near Chandigrah who provide me such practical training. Waiting for ur reply
It’s for the ease of use .. as everytime we develop primary antibody against the protein of interest in mouse preferably.. we don’t have to tag it with enzyme .. and the secondary antibody is going to bind to the constant region of the antibody .. so mostly .. the fab region of the antibody only is going change .. and the constant region doesn’t vary between different clones of antibody .. so the secondary tagged antibody can be purchased which is commercially available .
@@suganyasivakumar6972yes, that I can understand. But my question was simple, can we use direct enzyme tagged primary Ab (if it commercially available/some company providing it by request!!)
Please go through this link for detail : www.thermofisher.com/in/en/home/life-science/antibodies/secondary-antibodies/secondary-antibodies-target-species/anti-mouse-secondary-antibodies.html
To access notes and practice questions, please join our Telegram channel t.me/baayo_official
Your simplicity with words to manipulate makes you out of the box ..Nailed..Bold .. Respect
Simple and classy way of explaining..... really you explained not just protocol but the concept behind it..thanks for the efforts......best wishes for upcoming videos.
How can you explain such a complex method into such simple language ❤
perfectly explained in lesser time, and i have understood all the concept in just 10 min that's amazing
Tomorrow is my presentation on western blotting technique in college, hope this helps :( I'm memorizing this 🙂
💀 ??
On of my best channel simply explanation from Pakistan 😊
Best teacher in the world mam😎😎😎😎
🤗 love love
Incidentally i saw ur video.. and now i m ur fan.. Well explained..u doing such a nyc work for hindi medium students.. Like me..thanks a lot. ☺️🙏🙏
Thank you Akhilesh..
I also
This video is really amazing and helped me to understand the western blot. Thank you very much 😊
Thank you madam. You told the theory part. I will be very happy if you show the practical also.
Thank you for such an easy and accurate explanation.
Sorry to saying it im not flirting with you but seriously i cant concentrate on concept while you studying bcoz of ur beauty ....sorry n thank you for giving us valuable information
Thank you
THANK U
VEY MUCH
GOOD UNDERSTANDING 🙏👌👌🙂🙂
I can understand your language very easy.... Thank you so much ma'am 🥰🥰
1. Isolate protine
2 sample preparation
3. Sds page
4. Transfer on blotting pape
Nitrocellulose and PVDF membrane
Nice way of teaching ❤❤ appreciate able ❤
Thanks mamm you explained it shortly but the way of your explanation is attractive and best......
Ma'am thank you. App bahut achche se samjhati h aur samjh me bhi asani se aa Jata h. Mai Aapke Sare video dekhta hu. Ma'am Corona virus pr bhi video banaiye.
Good afternoon ma'am. Welcome again after long time.
Thanks Uday😊
Wowww maza aa aagaya🤩🤩 bss mam vo Bata dijiye anti rabbit anti body use karenge uska mtb kya h ??
MAm m aapse Bhut pehle se padh rha hu.. Aaap Bhut accha Pdhate ho
your teaching style is too good & u explained them all very well👌
Great Video Ma'am for explaining in the easiest way !
You helped me a lot...thank you so much..💗🙂
Thank you mam bottom of my heart
again thank you so much for all your videos
Please do make videos on molecular cloning
thanks mam for such a nice video.. you explain in very short and sweet way always. please make some videos on antibiotic resistance,and its types of efflux pumps.
thanks again.. keep up..
Noted Geeta..
Thanks for appreciation 🙏🏻😊
Too much easy explanation
Mam do min to apko dekhne mein hi chale jate hain phir dhyan aata hain padhna bhi hain . Just joke yes i understand your concept easily
Keep uploading ..
Oooooooo I found, what I was searching, thnx a lot mam, great
i love your lecture and the best part is you explain in such a way that everyone can understand
Thanks Saahikanta 😊
Thank you very much Ma'am! 😊
Nice maam.. Apke vedio se bht help milti h.. App microbiology se h maam ki biochemistry se
Mam thank u very much ur way of explaining awesome
Thanks 👍
Thank you too
Such a nice and informative video
Very good ma'am, thank you so much Ma'am.
Thank you Mam
You are Gem✨👍
Good teacher ❤❤❤lov from Pakistan ❤❤
Nice ma'am .. plz make a video on cell cycle regulation
Sure..will try to upload before 16th june..
Thank you so much ma'am
Mam, I want to know, is it require to again activate the PVDF membrane after the transfer?
Madam,what kinds of protein detection method can be used in western blotting ?
Amazing ma'am
Thanks bhuttt bda thankkuuu.....
i have a question. why proteins are transferred from gel to membrane? function of secondary antibody?
As always very much helpful mam
Mam app bahut aacha samjhati hai. Mam please bioinformatics ke topic per video banaiye
Mam i love u .... and your teaching technique ❤❣
Best of explain
Thanks. Please make video on Northern and Southern blotting as well.🙏🙏
Thanks alot✌✌..
Please make the lecture video on food microbiology's topics..
Thq mam ✨🎈
Thank you so much
Nice analysis mam
Thank you 😊
Ma'am can you explain on the topic 'schich test'
Very nice ma'am
thanks for your lecture
🇳🇵🇳🇵🇳🇵🇳🇵
Nice video
Thank you Narender 😊
BaaYo Dear Madam I had applied in NIPER for VU-VIS spectroscopy, GC and HPLC chromatography Practical training short courses but there is no replay from NIPER. Can u please help me to recommend other Institutions near Chandigrah who provide me such practical training. Waiting for ur reply
You are great
I am in class 12 this helps me a lot .....👍👍
Amazing mam
Really appreciated 👍👍
Madam you are amazing 👏 ❤ love you 😍
Very nice
Mam please make a video on eastern blotting
Thanks for this..❤️💥
Thank you ma'am 🙏
Thank you so much mem
Thnkew so much 💓
Mam you are the best thank you for your guidence mam I have a question what is the full form of BSA and mam please make a video on CCIEP Please mam
Bovine serum albumin :)
Thank you madam 😊
Ma'am real time pcr and rt PCR na videos hoy to please upload
Please biotechnology ka microscopes. bnaiyega
Why don't we make primary antibody labelled ?
Nice...
Thank u akansha 😊
Well explained 💜 from 🇵🇰🇵🇰
Very very Thanku mam
Too good mam😚😚😚
Mam plz let's know difference between tris base and tris cl.
This video of u is different your voice is more energetic
Than your before videos
Nd looks like u were excited!
So you find it good or not?😊
I dont know but your serious voice i liked that nd ur cool apperance i guess😅in older videos
Mam I hv lil bit queries like Why we don't use Enzymes tagged primary anybody instead used of secondary anti primary Ab!!??
It’s for the ease of use .. as everytime we develop primary antibody against the protein of interest in mouse preferably.. we don’t have to tag it with enzyme .. and the secondary antibody is going to bind to the constant region of the antibody .. so mostly .. the fab region of the antibody only is going change .. and the constant region doesn’t vary between different clones of antibody .. so the secondary tagged antibody can be purchased which is commercially available .
@@suganyasivakumar6972yes, that I can understand. But my question was simple, can we use direct enzyme tagged primary Ab (if it commercially available/some company providing it by request!!)
Informative 😊
Mam give me a video on ELISA TECHNIQUE
platelet aggregation test maam ,can u plz share
Can u please explain this little slowly? It's too fast. I need to pause everywhere n revise it. But ur contents are good
Why don't you try a feature called *Playback Speed*
Aap speed slow kr k dekhiye....
And here I am watching at 2x 😂😂😂😂
Thanks mam...
Thank u Sonali 😊
Mam please make on video of michaelis mention equation( most imt part of enzyme).
thank you mam
Ty 💥
Mam lc-ms pe bhi video bnaye n.
What is the limitation of this technique mam ???
Anti mouse ya anti Rabbit kya hota hai wese
Thnx
Hplc k interview questions pe eak complete video banaye pls.
Mam far western blotting and eastern blotting and zooblotting plz explain
Mam wat is antimouse antibody...and how these are developed
Please go through this link for detail :
www.thermofisher.com/in/en/home/life-science/antibodies/secondary-antibodies/secondary-antibodies-target-species/anti-mouse-secondary-antibodies.html
Mam microscopes ka video bnaiye
Mam Gene tagging ka bhi class lejia please