I tried the dipstick method now for a few times with my brand new Bentolab on both fresh and dried mushrooms and it works surprisingly well! I got high quality sequences (from Macrogen) even without a cleanup step. I do centrifuge for a few minutes before 'dipping' though, to prevent gettting bits of debri stuck on the dipstick, so it does take a bit longer than 30 seconds 🙂 The only problem I have is that the dispstick seems to absorb quite a bit of mastermix.. and if you start with only 20 microliters mastermix, and use 5 microliter for electrophoresis it is getting borderline for sequencing.
Hello! We haven't tried the dipstick method ourselves for DNA extractions from whole blood but from other tests we have done, we would suggest making the blood spot on filter paper and then using a ~3mm section of bloodied filter paper as your "tissue" for the dipstick extraction.
What sort of protocol are you thinking of using it for? It depends on the end use. It works well for anything PCR-related, but it definitely has its limitations, mainly that it doesn't concentrate the DNA like a column method would. There's a section on it at the bottom here: bento.bio/product/dipstick-dna-extraction-kit/
Hi Cristina, we would recommend using a fresh 1ml of wash buffer per sample. The idea is to wash off possible contaminants from each DNA extraction so reusing the wash buffer would introduce the possibility of transferring contaminants between samples. Our Dipstick DNA Extraction Kit contains sufficient wash buffer to have a fresh 1ml per sample: bento.bio/product/dipstick-dna-extraction-kit/
I tried the dipstick method now for a few times with my brand new Bentolab on both fresh and dried mushrooms and it works surprisingly well! I got high quality sequences (from Macrogen) even without a cleanup step.
I do centrifuge for a few minutes before 'dipping' though, to prevent gettting bits of debri stuck on the dipstick, so it does take a bit longer than 30 seconds 🙂
The only problem I have is that the dispstick seems to absorb quite a bit of mastermix.. and if you start with only 20 microliters mastermix, and use 5 microliter for electrophoresis it is getting borderline for sequencing.
can u explain the procedure if we want to extract the dna from whole blood using dipstick method? thank you
Hello! We haven't tried the dipstick method ourselves for DNA extractions from whole blood but from other tests we have done, we would suggest making the blood spot on filter paper and then using a ~3mm section of bloodied filter paper as your "tissue" for the dipstick extraction.
This is really amazing!! How's the quality of the DNA extract for tissue samples?
What sort of protocol are you thinking of using it for? It depends on the end use. It works well for anything PCR-related, but it definitely has its limitations, mainly that it doesn't concentrate the DNA like a column method would. There's a section on it at the bottom here: bento.bio/product/dipstick-dna-extraction-kit/
@@BentoLab For PCR. Thanks for the link! Do you have recent data where you used whole blood instead of tissue samples?
Are you able to use the wash buffer for multiple samples, or is 1 ml needed per separate sample?
Hi Cristina, we would recommend using a fresh 1ml of wash buffer per sample. The idea is to wash off possible contaminants from each DNA extraction so reusing the wash buffer would introduce the possibility of transferring contaminants between samples. Our Dipstick DNA Extraction Kit contains sufficient wash buffer to have a fresh 1ml per sample: bento.bio/product/dipstick-dna-extraction-kit/
This is really amazing 🌹🌹🌹❤️
Thank you!