Hello sir, sir i have tried this with my experimental data where the max concentration i used was 40IJ/ml and after calculating the LC50 which was 64.93. sir how can be the LC 50 value (which is 64.93IJ/ml) MORE THAN the concentration i applied i.e., 40IJ/ml. kindly clear this doubt sir. should it not come between the range of the concentration i used for the experiment.
All the values of x are basically Log value. Therefore to get the LC50 value we must convert it by Anti-log & finally we get the value in mcg/ml or any other unit.
RESECTED SIR, THANK YOU EASILY YOU EXPLAINED THE PROCESS OF FINDING LC 50. IT WAS VERY USEFUL
Appreciate sir. Respect form Pakistan.
Thank you sir, It was very helpful. Informations given are very clear
For any other information/ support mail samratbose09@gmail.com..
Thank you. Nice and informative video for Bio-assay experiment
You are most welcome
What is the probit value for 0 and 100 percent mortality rate?
Please describe.
for lc50 of rohu embryo also the value of y in the formula y=ax+b will be 5 ?
Respected Sir, Can you please let me know how to find LCL and UCL over here
Respected Sir, Could you please help me in computing LC90 in a similar way
sir, is it possible for you to share the excel file?
Hello sir, sir i have tried this with my experimental data where the max concentration i used was 40IJ/ml and after calculating the LC50 which was 64.93. sir how can be the LC 50 value (which is 64.93IJ/ml) MORE THAN the concentration i applied i.e., 40IJ/ml. kindly clear this doubt sir. should it not come between the range of the concentration i used for the experiment.
How did you calculated LC50=Antilog x? I don't understand this video that Antilogx
All the values of x are basically Log value. Therefore to get the LC50 value we must convert it by Anti-log & finally we get the value in mcg/ml or any other unit.
Log 10 calculating is very simple.
is this method used for adult zebrafish also ?????
No. It is only for Embryo.
Sir how the no.of fishes is getting dec with the time duration
If your test drug is toxic in nature then with time the death rate will be higher.
Thank u sir🙂
Sir, in the equation y=ax+b you took y as 5. Why did you take it as 5?
50percent probit value is 5.0
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