Microbiology 03 = History of Microbiology | Microbiology Scientist | Development of Microbiology

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Contribution of a scientist -
(1) Antonie van Leeuwenhoek (1632-1723) was one of the first people to observe microorganisms, using a microscope of his own design, and made one of the most important contributions to biology. Robert Hooke was the first to use a microscope to observe living things. Hooke’s 1665 book, Micrographia, contained descriptions of plant cells. Before Van Leeuwenhoek’s discovery of microorganisms in 1675, it had been a mystery why grapes could be turned into wine, milk into cheese, or why food would spoil. Van Leeuwenhoek did not make the connection between these processes and microorganisms, but using a microscope, he did establish that there were forms of life that were not visible to the naked eye.
(2) Alexander Fleming had a genius for technical ingenuity and original observation. His work on wound infection and lysozyme, an antibacterial enzyme found in tears and saliva, guaranteed him a place in the history of bacteriology. But it was his discovery of penicillin in 1928, which started the antibiotic revolution that sealed his lasting reputation. Fleming was recognized for that achievement in 1945, when he received the Nobel Prize for Physiology or Medicine. Returning from holiday on September 3, 1928, Fleming began to sort through petri dishes containing colonies of Staphylococcus, bacteria that cause boils, sore throats and abscesses. He noticed something unusual on one dish. It was dotted with colonies, save for one area where a blob of mold was growing. The zone immediately around the mold-later identified as a rare strain of Penicillium notatum-was clear, as if the mould had secreted something that inhibited bacterial growth.
(3) Louis Pasteur (1822-1895) expanded upon Spallanzani’s findings by exposing boiled broths to the air in vessels that contained a filter to prevent all particles from passing through to the growth medium. He also did this in vessels with no filter at all, with air being admitted via a curved tube that prevented dust particles from coming in contact with the broth. By boiling the broth beforehand, Pasteur ensured that no microorganisms survived within the broths at the beginning of his experiment. Nothing grew in the broths in the course of Pasteur’s experiment. This meant that the living organisms that grew in such broths came from outside, as spores on dust, rather than spontaneously generated within the broth. Thus, Pasteur dealt the death blow to the theory of spontaneous generation and supported germ theory instead.
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