Microfluidics Interviews #2: Paper-based microfluidics
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- Опубліковано 15 чер 2024
- You don't need an expensive lab to do microfluidics! 🧬 In our last interview in this series, we learn how to make low-cost viral screening tests on paper chips with Laura Magro, PhD!
↓ More infos and links in the description ↓
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LINKS:
French version: • Interviews Microfluidi...
Subscribe to the channel : / thelutetiumproject
Follow us on Twitter : / theluproject
Visit our website: www.lutetium.paris/en
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MORE ABOUT THE INTERVIEWEE :
Laura Magro is an alumnus of ESPCI Paris, and of Université Paris Diderot, where she obtained a master's degree in Complex Systems, with a major in Microfluidics. In 2016, she defended her thesis, titled "Paper microfluidics : from liquid flow studies to Ebola virus diagnostics in Guinea", that was conducted in the Microfluidics, MEMs and Nanostructures team of the Gulliver lab, at ESPCI Paris. She joined CEEBIOS in 2017, a company that promotes the development of biomimetic technologies applied to eco-responsible innovation. She has been the company's deputy director in charge of scientific development since 2018.
Website of CEEBIOS: ceebios.com/
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FEATURED ARTICLES:
Laura Magro, Camille Escadafal, Pierre Garneret, Béatrice Jacquelin, Aurélia Kwasiborski, Jean-Claude Manuguerra, Fabrice Monti, Anavaj Sakuntabhai, Jessica Vanhomwegen, Pierre Lafayee & Patrick Tabeling, Paper microfluidics for nucleic acid amplification testing (NAAT) of infectious diseases, Lab Chip, 2017,17, 2347-2371
pubs.rsc.org/en/content/artic...
Laura Magro, Béatrice Jacquelin, Camille Escadafal, Pierre Garneret, Aurélia Kwasiborski, Jean-Claude Manuguerra, Fabrice Monti, Anavaj Sakuntabhai, Jessica Vanhomwegen, Pierre Lafaye & Patrick Tabeling, Paper-based RNA detection and multiplexed analysis for Ebola virus diagnostics, Scientific Reports volume 7, Article number: 1347 (2017)
www.nature.com/articles/s4159...
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VIDEO BREAKDOWN:
00:00 Microfluidics: too unpractical?
01:34 Advantages of paper-based microfluidics
02:23 Simple examples of paper chips
05:22 Paper-based diagnosis
06:18 Pathogen detection methods: an overview
07:17 Advantages of nucleic acid testing
08:13 From the lab to the field: Ebola testing
09:10 Towards diagnoses on smartphones?
10:18 Conclusion
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CREDITS :
Host:
Mathias Kasiulis
Researcher:
Laura Magro
Writers:
Guillaume Durey, Mathias Kasiulis
Director:
Hoon Kwon
Animations:
Hoon Kwon
Editing:
Pierre Dupont, Guillaume Durey
Studio, Visual Identity:
Juliette Nier
Theme Music:
Pierre David
Production:
Guillaume Durey, Mathias Kasiulis
This video was shot on May 17th, 2016.
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The Lutetium Project is a PSL students’ initiative conducted as part of IDEX ANR-10-IDEX-0001-02 PSL and funded by:
PSL Research University - www.univ-psl.fr
ESPCI Paris - www.espci.fr
Espace des sciences Pierre-Gilles de Gennes - www.espgg.org
ESPCI Alumni - espci.alumni.paris
le Fonds ESPCI Paris - www.fonds-espci-paris.org
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Excellent work! The Lutetium project has done an excellent job of showcasing modern research in an elegant and approachable method. I look forward to the upcoming work!
Hi there! Two things:
1. English subtitles are available in the settings menu of this video. Click "CC" to the left of the gear icon on the lower right of your UA-cam player.
2. This video was shot in the spring of 2016, and constitutes the end of our series on microfluidics. You can learn more about what Laura was up to in the years before and after we shot this video in the description. Her PhD studies laid the groundwork for the rapid development in 2020 of COVID-19 diagnostic kits using paper-based microfluidics: www.espci.psl.eu/en/news/2020/a-new-generation-of-molecular-testing-for-covid-19.
Enjoy!
Where's the paper in this new system, seems like cheating and that they backstepped to what we already do. And they have been working on these things for at least six years (not covid specifically but still) and this is what they have to show for it. Plus they only made around a thousand TO BE USED IN LABORATORIES which defeats the entire point of these -paper field- tests
To me on the outside, and a little inside being pre-med (technically Cell-bio & Neuroscience with biomedical options just to flex, also going for biomedical eng masters afterwards, then something probably with computational biology)... this is disgraceful
And they ought to be ashamed of how impractical and wasteful this all has been, without even getting any kind of promise for meaningful use
Not trying to be more of an ass, but if they were smart they should know this will probably never go anywhere beyond "proof of concept". And if they do, then they are just coasting on grant money which is the pinnacle of evil in science imo
But please I would love to be proven wrong thoroughly and completely by them coming out with anything that actually gets used
Amazing! I thought that the folded wax paper microfluidic devices were excellent, but the other things mentioned, such as using a smartphone for electrophoresis and diagnostics is even more exciting. Excellent work! I hope we can see more of this technology being used in the near future.
Amazing! Great stuff, it blows my mind. Thanks a lot!
The work seems very amazing. Keep exploring
Speechless 🙊
Very interesting for sure!
I wonder how these mixed paper+electrical microfluidic circuits look. For example, is there a way to make a small valve? That would give a bit more control to, say, let a reaction sit for a while in a small reservoir before continuing through another chamber, or to let reactions take place at specific timesteps.
Oh, and of course if there are plans to have an on-paper PCR?
In theory, with a phone-based heating element and the right materials (even including the gel strip with electrodes, if it can be added to the paper), it sounds doable, and could be very exciting!
It was an extremely informative video, Thank you so much. well I wish to know what paper did she used tp make those ?
Very exciting work. Can you help connect with the lab
Hi, this video is quite informative and very interesting. is it possible to know the brand of ink that Laura used? Thank you
She claims to not need other equipment besides a modified phone type device, but literally in her paper it requires a suitcase that has a heater (probably a cold compartment too) and an "optical system of detection". This is nothing new, and we certainly have the same tests in the same or smaller size factor (putting shit in a suitcase)
She probably means to replace the suitcase with the phone device, but again this is certainly something that's already done (piezoelectric plates and a single UV LED)
As a pre-med student these seem very impractical and short sighted, possibly even idiotic
Clearly the only tests that can be done are qualitative (positive/negative/control)
Doing a single test on one layer makes sense, because it is simply one layer, but the reason there are not significantly more of these types of tests is imo stability and accuracy
How do they stop the paper from filtering nearly everything not dissolved directly in the liquid. Say you wanted to test urine with multiple assays, how would you be able to test protein levels, salt, and glucose. The protein would stay on the top layer/input hole and can only move on the surface level, while the sugar is incorporated into the paper (as cellulose is a sugar they will "stick" together), and the salt is diluted throughout the paper as it absorbs the water; and looking it up it seems celluloses charge causes pockets of charge (not uniform) along with sometimes seeming to prefer one ion over another. There doesn't seem to be any way to avoid these pockets due to how it's processed. These unpredictable and unavoidable pockets would make it impossible to accurately determine ionconcentrations, or anything else with charge as well. Leaving an insane amount of room for false negatives, especially from all this hugely variable saturations
Also, nucleic acids are famously unstable (especially at room/surrounding environment temp) and are often very messy when it comes to binding (which your cells fix them all the time by having tons of them, and multiple ways of detecting when they aren't binding as they ought to, and also multiple ways of either correcting it or simply decoupling them). How is it possible to prevent, or even better, correct missbound sites. How do you prevent false positives, especially if it happens to be an especially "sticky" acid
There is no "logistical" way I can see where you can ship these without destroying it in the process, as well as contamination being very likely (likely that random bits from organisms will find there way to the testing site because that's just field work)... false positives for days
And one of the biggest problems I see is temperature, in the field it's usually difficult to keep things at the temp they need (most being below room temp, or at body temp). You wouldn't be able to do tests at higher temps (PCR is good example @Xasin) as the wax would melt again and contaminate everything. And if you're bringing equipment to control temp, then it is simple enough to bring most everything else, as they usually come hand in hand for field testing
They only viable way I can see this actually panning out, is how it already has. Which is use highly stable compounds (say antibodies) that then react to release a dye or become fluorescent. And again the ones that already exit (not just pregnancy but drug tests too, and ) are generally just good enough for the most simple of tasks. Trying to make it more complicated seems like nothing but adding more failure points for a very small gain of yes or no. Especially if your trying to detect with things that have an extremely variable response, such as testing immunocytes or nucleic acids
TLDR: i don't see how this is better than what we have. The only way this is feasible (IMO) is by doing what has already been done, and anything more is just not reliable in any meaningful way. But I am just a pre-med, so if she and her team have figured out how to overcome all of these things (and likely significantly more) then good on her. I am very cynical on this, but I would love to hear how any of this can be done, both easily and on the cheap since that's the entire point
@RustyCobalt The goal is never to prevent false positives but to minimise it (conventional testing takes time, instruments, skilled manpower. Remember this is for rapid diagnostics only. Can always be followed up with precise tests) and regarding maintaining temp for nucleic acids, you can always carry it in a ice basket. Also remember paper microfluidics is not the solution to all kinds of testing. Since you are pre-med, good luck!
@@ritobratochatterjee7358 Thanks for the luck, definitely need some
But too your point, if your going through the effort to bring that equipment you might aswell bring the ones that work constantly and repeatedly
The same reason to bring that ice basket
And yes, whether they be false negatives or positives the goal can only be to minimize really, more so negatives for that same reason of following up