Mam you are awesome you teach so good the introduction and the point are so good please mam provide some notes for your handwriting notes in you telecom channel you like that is so good I am very glad
Progressive staining is a slower staining process in which the tissue is left in the staining solution just long enough to reach the desired endpoint. In contrast, regressive staining is a more rapid staining process in which the tissue is deliberately over stained and then de-stained.
Eosin is very soluble in water and diluted ethanols. So if you do first Eosin staining and then go through aequous, acid hemalaun (pH = about 3), then bluing for a few minutes, no Eosin-staining would be left. It is the same, as if you let your already stained slides sit too long in the rinsing water and dehydrating ethanols. Eosin will be gone. Try it.
Here is my protocol. It works in paraffinsections (5-10µm) as well as in mounted frozen sections (10-30 µm). Dewaxed and defatted sections rinse in destilld water. 1. Mayer's Haematoxyline (0.1%) - 5-10 min; 2. rinse well in destilled water; 3. blue in running tape water for 10 min., 4. 1% alcoholic Eosin- solution 1-5 min,; 5. rinse in destiiled water; 6.diffenciate in 70% ETOH (check under the microscope) and 2x 100% ETOH (10 dipps each alcohol); 7. Isopropanol 2 min; 2 x Xylene 5-10 min each; 8. mount in Depex or another xylene soluble mounting medium. Start with a test slide to optimize your stainig times an control after every stainig step the quality under the microscope.
Brother if u have watched the video very carefully the answer of your question is given at 15:33 ,It takes approx. 70-75 minute for the whole proceudre to take place Thank u 💓
Very good explanation I am MBBS student 3rd yr but I like ur way of teaching stay blessed sister
I am glad that you are appreciating my effort
Best
Glad
महत्वपूर्ण जानकारी देने के लिए धन्यवाद!🤘😄
My pleasure
@@MedicaLabtechSubhra tqqqqqqqqqqqqqqqqqq
My pleasure
I understood everything.Thank u
😍
Purple🧞♀️ amazing lecture
Aapki wajah se mene apne practical m phod diya...thanks bro😀😀😀😀😀😀😀😀😀
Inspired with your comment.glad to hear
Very helpful thanks for the video 🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏
I am glad it is helping you
Mam please continue your teaching you will be the great teacher of DMLT and bmlt student salute mam
I am glad with your comment.thank you for inspiring
Mam you are awesome you teach so good the introduction and the point are so good please mam provide some notes for your handwriting notes in you telecom channel you like that is so good I am very glad
Thank you for inspiring me with your words.Handwritten notes will be available soon
Wonderful Full explanation dear 🤗🤗
i m glad
Thankyou so much dear 🤗
i am glad
NYC 👌
Ty
V good way of delivering lecture..
V nice👌👌👌👌
i am glad it is helping you
Plz ap nissl staining py bhi presentation dain
I will surely work on your suggestions
My all problem solve histology sub. Thanks alot mam👌👌🙏🏽
I am glad it is helping you
My concept clear TNX so much🤗
I am glad it is helping you
You are speaking Hindi very well, now I am very happy to hear that.Dear😄🤗
trying to make it more comfortable.Thnx for d Compliment
@@MedicaLabtechSubhra welcome my dear 🤗😄❣️
My pleasure
I was not understanding this topic v well.. But u described excellent.. Thanks
Very well explained mam
May God bless you 💓
I am glad & inspired with your comment
Best video..thanks mam...💯
👌🏻👌🏻
😍
Keep going it's good lecture 😉
Your comment gives me inspiration to make more videos like this
Thanks alot mam
My pleasure
Why hemotoxylin is added in running water and why not in dustilled water?
Nissel staining.. Cresyl voilet py plz presentation dain
Mam ye wala lectures samj nhi aya ky aap ye dubara bana sak te ho
I love u mam 😂since I have exams u saved me all concepts are cleared ❤️
I am glad it is helping you
Apko ssb ka paper hai
Mere pas ssb ka paper nhn h
@@MedicaLabtechSubhra ok mam
Mam please difference between progressive and regressive haemotoxylin staining prr video bna do mam please please mam
Progressive staining is a slower staining process in which the tissue is left in the staining solution just long enough to reach the desired endpoint. In contrast, regressive staining is a more rapid staining process in which the tissue is deliberately over stained and then de-stained.
Which mordant to add to hematin...?
Salts of aluminium is more useful
Mam very helpful video but background noise nhi hota to accha hota
Thank you.I will surely try to work on your suggestions.
What will happen if you use eosin at first followed by hematoxylin stain..?
Madam.. Please give me answer
Eosin is very soluble in water and diluted ethanols. So if you do first Eosin staining and then go through aequous, acid hemalaun (pH = about 3), then bluing for a few minutes, no Eosin-staining would be left. It is the same, as if you let your already stained slides sit too long in the rinsing water and dehydrating ethanols. Eosin will be gone.
Try it.
Madam,, how I will confirm the quality of absolute alcohol?
By tasting with tongue 😉😉😉😉
mam histopathology ka kuch main question mil shakta hai kya
exam se 1month पहले hum discussion karwange..UA-cam pe
@@MedicaLabtechSubhra hn main dmlt 2 nd year Ki student hun or mera last semester bacha hai I don't know exam hoga kab
aap mujhe instagram.com/subhras__biology?igshid=blr77efm9ofj
Follow kr lijiye.if i can help
@@MedicaLabtechSubhra maine bahat pehele hi follow kardiya or thank u etna acha video banane ke liye
How much time is required to prepare H and E slide for reporting?
Here is my protocol. It works in paraffinsections (5-10µm) as well as in mounted frozen sections (10-30 µm). Dewaxed and defatted sections rinse in destilld water.
1. Mayer's Haematoxyline (0.1%) - 5-10 min; 2. rinse well in destilled water; 3. blue in running tape water for 10 min., 4. 1% alcoholic Eosin- solution 1-5 min,; 5. rinse in destiiled water; 6.diffenciate in 70% ETOH (check under the microscope) and 2x 100% ETOH (10 dipps each alcohol); 7. Isopropanol 2 min; 2 x Xylene 5-10 min each; 8. mount in Depex or another xylene soluble mounting medium. Start with a test slide to optimize your stainig times an control after every stainig step the quality under the microscope.
Brother if u have watched the video very carefully the answer of your question is given at 15:33 ,It takes approx. 70-75 minute for the whole proceudre to take place
Thank u 💓
How to prepare percentage of alcohol,like 90%,80%,70%....etc mam let me know the method correctly..
70%alcohol - Suppose To prepare 100ml of 70% alcohol .
Add absolute alcohol (100% alcohol)
70ml & add 30ml of Distilled water.
(70% absolute alcohol + 30ml distilled water.)
Thank u...
Congratulations mam 🍫😆 1000 subscriber complete.
Thanks a million
Bluing hum kyon karte hai thoda achha se bata dijiye
Hydration kyu kar rahe h
Hydration means addition of water . you have to hydrate the tissues so that they can absorb stain. Easily.
App ka qualification kya ha
Dmlt
Cresyl voilet
Please..........in English
I will surely try to