Autoclave | Sterilization Process of Glassware and Nutrient Media by Moist Heat Sterilization
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- Опубліковано 29 вер 2024
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AUTOCLAVE
The autoclave is a container similar to a pressure cooker. It is filled with the objects to be sterilised and then sealed. Next, high-temperature steam is forced in under high pressure, thereby displacing air. Moist heat destroys microorganisms by the irreversible coagulation and denaturation of enzymes and structural proteins. The time and temperature to accomplish this depending on pressure and the type of microorganisms to be inactivated. After the necessary time has passed, the steam is released and the sterilised objects are removed. The entire cycle can take from 15 to 60 minutes (batch processing). pushpendra patel solution pharmacy. vandana janghel solution pharmacy. A more commonly used method when extended heat is not a concern is to use an autoclave or pressure cooker.
PRINCIPLE
The basic principle of steam sterilization, as accomplished in an autoclave, is to expose each item to direct steam contact at the required temperature and pressure for the specified time. Thus, there are four parameters of steam sterilization: steam, pressure, temperature, and time. The two common steam-sterilizing temperatures are 121°C (250°F) and 132°C (270°F).
Moist heat destroys microorganisms by the irreversible coagulation and denaturation of enzymes and structural proteins. In support of this fact, it has been found that the presence of moisture significantly affects the coagulation temperature of proteins and the temperature at which microorganisms are destroyed.
Uses
Steam sterilizers also are used in healthcare facilities to decontaminate microbiological waste and sharps containers. Autoclave is particularly useful for media containing water that cannot be sterilized by dry heat. It is the method of choice for sterilizing the following:
1. Surgical instruments
2. Culture media
3. Autoclavable plastic containers
4. Plastic tubes and pipette tips
5. Solutions and water
6. Biohazardous waste
7. Glassware (autoclave resistible)
PROCEDURE
1. Place the material to be sterilized inside the pressure chamber and fill the cylinder with sufficient water
2. Close the lid and put on the electrical heater.
3. Adjust the safety valve to the required pressure.
4. After the water boils, allow the steam and air mixture to escape through the discharge tap till all the air has been displaced
5. This can be tested by passing the steam-air mixture liberated from the discharge tap into a pail of water through a connecting rubber tube.
6. When the air bubbles stop coming in the pail, it indicates that all the air has been displaced by steam.
7. Close the discharge tap. The steam pressure rises inside and when it reaches the desired set level (e.g. 15 pounds (lbs) per square inch in most cases), the safety valve opens and excess steam escapes out.
8. Count the holding period from this point of time, which is about 15 minutes in most cases.
9. After the holding period, stop the electrical heater and allow the autoclave to cool until the pressure gauge indicates that the pressure inside is equal to the atmospheric pressure. Open the discharge tap slowly and allow the air to enter the autoclave.
10. Open the lid of the autoclave and remove the sterilized materials.
This video will help you to understand- autoclave working, the principle of an autoclave, sterilization process, the importance of sterilization, sterilization process, moist heat sterilization, working of an autoclave, construction of autoclave, how autoclave works,
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Thankyou ma'am very informative video
For what I was looking for 😀😀
Very Good information about Autoclave, I can clearly understanding Thank You So Much😊
Thank you very much
For Your most Valuable video
Very good this is video I understand methods of autoclave👍👍
Thanks for your information 🙏
Thanks ma'am
Thank you. 🙂
Thank you
Very very useful for me...I saw many videos on UA-cam to understand the autoclave...but no one has made this topic easiy as u did...🥰🥰🥰🥰
Thank you so much for your kind feedback
Great information
it was good
Thanku very much mam🙏
Good translation mam.
Thank you
Well explained thanku mam
Scissors and forcep autoclave kr skte hai kyya...plss ans 😢
Thanks for explaining ❤️❤️ can you answer me why we cover baeker and petridish??
To prevent from salt depisition
Mam incubator me cabinet hota hai but autoclave cabinet q nhi hota please give me ans
Thanks mam
Please make videos in English
m Hemanth Its also available in english
Please put link
Your video s are good
m Hemanth Autoclave - Moist Heat Sterilization in Microbiology by Solution-Pharmacy in English ua-cam.com/video/y_EhCERWzfs/v-deo.html
Are conical flask and patri dish empty
Peyri dish is empty
Mam i have a doubt .....steam releasing valve and air outlet valve are same or not?.......and what about ventcock?
Mostly its depends on manufacturer. But their functions are same
What happens when we donot cover the glass wares with paper during sterilization?
Sediment will deposit under glass
To is ko dry kese karen gy???
I thought this was supposed to be in Hindi
Isnt it ?
who here after autoclave herring sperm dna😂
Very simple and clearly explained. Thankful for the detail and ease of use
Thanku
We also need to cover the cotton plug (using foil paper, etc) as well, unless it well moist.
Se
Tujhe bohat mlm hai
Incubator chalane,knowledge
Very helpfull lecture thanks ser ❤❤🙏🙏
Very good exlpanation 👌👌
Thanks so much ..
3.21 cfu/g is my reading
.: Then how many colonies are there
Good
M 15 m ane k bad switch batn off krke 20 min k lie wait krte h ky please reply
Thanks for this kind explanation but ma'am can we apply this method in Pharma side or not .
I am fresh pharmaceutical Microbiologist
Yes
Thanku you
But i don't seen there is no chemicals indicator used,, how to identify ,,successful completely sterilization the Instruments?? We have to use the 3m chemicals indicators,,,
This was demo as per syllabus. So we didn't use indicators
Thnku mam
Wow
Wow
Thnx mam
Conical flask ko pura cover q nehi kiya Gaya?
Good explanation with practicals
i like
Ooosssm
Thank you mam very nice explain 👍👍
Thank you for this information 😊
Glass items are sterilized in hot air oven
❤
Easiest video ever to understand working of autoclave tnxxx ma'am:)🥰🥰🥰
Pls tell me how to maintain 15psi temp?
Only for cssd staff ko puri jankari hogi
Good presentation 👍
Is this for d.pharma or b.pharmacy🤔
For all
Thankx for information 👍
Thank you 😊😊 Superb 👍👍👍
This video clear my concept 😊
Good
Thank you so much ❤️ mam...... nice explanation...👌👌
Pleasure
full form of psi is pound square inch....not a per square inch
Awesome explanation ma'am thanks
Thanks mam
Fantastic 🎉
Thanks 🤗
Best. Video mam..
Thank you❤😍
I like ur video mam.... Aapne bde ache trike se btaya sb
Bahut bahut thanku mam
Thanks a lot for making this video ma'am
Amazing explaination mam 👏👏👏
Thank you for useful information
Thanks for the video in my auto clave i have one more option "vaccum release valve" can u pls tell me what is tge procedure for that
R.Naganath Davey That is used for releasing a small amount of vapour so that vaccume may not created.
Which make you have autoclave
Thank you mam for explanation....🙏
mam 15 lbs k upar pressure jane k baad whistle apne aap bajne lagta hai ya use manual karna padta hai please reply
Thanks for sharing the video
Why brown paper is used black or white nii. Please tell
You can use white or aluminum foil too
Thnku so much 🤗🤗 it's really very helpful......👍👍👍👍👍
Thank you
Thanks mam.
Thanku so much
Very nice
What is in flask in the form of liquid plz reply
Nurient agar media
Nicely explained. Thanks. I need to sterilize a metallic object duly sealed in a pouch. Which pouch I can use for this purpose
😂
Good evening mam
🤗😀👌🥰❤️
Very helpful procedure tq u....
very nice and informative vdo
U explained everything be smoothly
Thank you
Does moist heat sterilisation destroys nutritional value of fruit juice?
If it may affect by heat
Thank you mam
Mam plz i wanna these video in totally english
It is also available in English. Check description box and channel
Ma'am can you please tell me why we didn't cover flask with paper
Mem hitting joint may lik hoya kiya souliotion hai? Same autoclave.
I wanna these video in english language plz mam
Check description box for english link
Nice information 👍
Thank you
Thankyou mam
Mam ik bat puchni hy kya hum is main Daal b pkaa skty 😂
Ha par isse aapke exam ki khuchdi ban jayegi
Kya autoclave me koi chemicals bhi milaya jata hai
Nahi
Thank u mem🙏
How to maintain 15 lb/inch for 20 minutes. After reaching once 15 lb/inch.
Appreciate ma'am
Blood bag autoclave kasise kare sir new video banana sir
Thank u mam
Tq man😘😘
Bahut khub maim
Bahut sahi tarike se bataya maim ne 💯☝️😊👌👌👌👌👌