That's not accurate. For competitive lateral flow, two visible lines indicate a negative result, whereas a single visible line (on the control region) indicate a positive result. The video above could be applicable to noncompetitive lateral flow. The animation looks great though, thanks!
Thank you for your comment. We absolutely agree that binary competitive lateral flow tests, the test line typically disappears when the analyte exceeds the detection limit. The major difference in this example is that Neogen’s test kits are quantitative and use the Raptor instrument to interpret the result so rather than absence of a line, the raptor reader measures the ratio of intensity between the test line and control line to produce a quantitative result based on a lot specific standard curve that is pre-loaded. Because there is an inversely proportional relationship between signal intensity and target analyte. (line gets lighter as the test gets more positive), this would still be considered a competitive lateral flow. Please continue to follow our channel for new videos!
What happed when the sample is overloaded with toxins? What may course the disappearance of the control line?
That's not accurate. For competitive lateral flow, two visible lines indicate a negative result, whereas a single visible line (on the control region) indicate a positive result. The video above could be applicable to noncompetitive lateral flow. The animation looks great though, thanks!
Thank you for your comment. We absolutely agree that binary competitive lateral flow tests, the test line typically disappears when the analyte exceeds the detection limit. The major difference in this example is that Neogen’s test kits are quantitative and use the Raptor instrument to interpret the result so rather than absence of a line, the raptor reader measures the ratio of intensity between the test line and control line to produce a quantitative result based on a lot specific standard curve that is pre-loaded.
Because there is an inversely proportional relationship between signal intensity and target analyte. (line gets lighter as the test gets more positive), this would still be considered a competitive lateral flow. Please continue to follow our channel for new videos!
@@NeogenCorp Thanks for the clarification! Nice work!