How to Remove Carryover in HPLC?

[@斗战胜佛-k7b]

With regards to the Hot Water Purging. is there any guideline on how hot should the water be and also how long we should purge the whole system at what flow rate?

[@azam1065]

Sir, i think one more point is important which is called gradient selection, it should equilibrate with mobile phase for some time called post run with appropriate solvent.

[@xiaowei379]

Hi sir, my HPLC column so easy carryover after 3-5 run of sequnces after higher concentration STD. Can you please advise me how to clean the column ? Im pretty sure is carryover on the column.

[@PRAVEENKUMAR-xb6sb]

sir diluent strength should not be more than mobile phase in which context??
it will came in stronger or weaker mobile phase or any other context.
pls gave more light on this ??

[@maheshpds9]

What is the criteria for online samples in RS analysis. That means how many hours before we can prepare sample solution.
Is there necessary to inject blank between samples and bkt STD

[@斗战胜佛-k7b]

May I know what OPA stands for?

[@lalitkhairnar4418]

Sir point no. 5, in RPC use polar compound so why THF to use remove the non polar compound

[@ask5755]

But in my case looks carryover but not carryover because lambda max of both totally different...what should I do

[@naveenrajubadde]

Thank u sir..Can I use diluent for needle wash....?

[@rajeshpudi8795]

You can use

[@praveennandaram9415]

Yes u can use

[@Ashok_pp]

Thanq sir

[@prakashbhingude4889]

Nice sir

[@sdscienceworld8531]

Hi.. your video is really helpful for me can you make a video regarding integration parameters for HPLC and Impurities integration 🙏🙏🙏
Thanks in advance...