Preparing umbilical cord mesenchymal stem cells from start to finish.
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- Опубліковано 19 бер 2023
- In this video Dr. Whitney describes and demonstrates the steps of preparing umbilical cord mesenchymal stem cells, and getting them ready for application.
While some parts are sped up slightly, this video provides a step-by-step glimpse at how promptly and mobile the preparation of stem cells can be with the proper set up.
To find out if regenerative stem cell medicine can help you or a loved one call Innate Healthcare Institute at 602.603.3118 or check out innatehealthcare.org
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Comment below if you or someone you know has had regenerative medicine! - Наука та технологія
Best video presentation I have seen, and I have been looking for this type of explanation/demonstration for the past six years.
Thank you for this straight forward presentation Dr. Whitney ! you deserve an award for truth and open applicable information. The deception is ended with this video.
Thank you! Very kind of you.
Thank you for healing people for real
There are many people that don’t know about these great treatments
May you can get some testimonials for your patients
Perfect timing for this video. We were going to call today to ask entire procedure. As we are educating ourselves, we have learned thaw is critical part of entire process, as well as experience in industry.
Glad it could help!
I had 120 million done and also received 4 cc of exosomes and PRP to shoulder.
Thanks for your reply!
Where are the cells sourced from? What hospital,etc. Is it similar to the quality of Stem Cells in Panama? If so why so cheap?
Please can make video on culturing them after thaw, its difficult know the right protocol
Thank you so much for watching our stem cell procedural video. We are in the process of making a video similar, about the culturing of the cells. Stay tuned.
Thanks for the informative documentary. I am a practicing MD who is planning to have MSC treatment delivered iv and intra-articular administration. I have a couple of questions for you. With open air mixing and handling not in sealed vials. I would feel much more confident in the sterility of the mix if you were wearing a sterile gown and cap and working under a ventilator hood to ensure no inadvertent contamination. Also what is the size of the MSC and what is the fate of iv administered MSC’s. Do they tend to clump at all and do they traverse the pulmonary capillary bed without getting trapped…then they would not be able to migrate to where they are needed in the body. Thanks again!
Thank you, MSCs run about 15-30 um in size. Typically there's a good amount of engrafting in the lungs. We use vasodialtors during IVs to attempt to get the cells through that lung passage. The prevailing thought is that even with lung entrapment the cells produce an abundance of biomolecules and are interacting with the hosts immune system that spread to the rest of the body.
What percentage of the 94% remains viable for 1 hour or longer? What’s the range in cost (even though you don’t sell them yourselves, you do buy them) to clinics and hospitals per vial, or if less expensive, per 12 vials? How many umbilical cords are used per 25 million cells, if you know?
If the cells aren't in saline they will die pretty fast. With saline they will stay good for 30 min. Around 2 hrs if in ice. Cost depends how many cells we're growing/culturing, which means more time and work. For example 300 million cells costs $11,500. We don't buy those cells, we buy the cords and grow the cells out ourselves. To get 25 million cells you'd only need around 0.1 inch of an umbilical cord.
Is there a treatment for optic attophy
Is there a training course to learn how to prep the stem cells and equipment? My doctor is extremely interested in this
Not that I know of. There's plenty of research papers that explain in their Materials and Methods section.
Hi doctor. First of all, Merry Christmas, and thank you so much for sharing your knowledge with us. I'm very interested in learning how to insulate mesenchymal stem cells out of an umbilical cord. I've been digging into those kits out there to extract stem cells and I had decided to buy one, but now I hear you saying those are no good at all, and for that reason I was wondering if you could point me in the right direction. Do you know where I could find a protocol explaining every single step of the process? And by that I mean all the chemicals, tools and equipment that I'll need in order to start insulating mscs. Doing my research, I stumbled upon a book named Mesenchymal Stem Cells Methods and Protocols, and another one named Insolation, Mesenchymal Stem Cells Insolation, Characterization and Applications. In case you can shed some light I'd really appreciate it. Thanks again for sharing your knowledge with us. Best regards from Santiago-Chile
Dr. Ricardo Nazal
Hi Dr. Nazal, there's lot of different protocols out there. Maybe start here: www.sigmaaldrich.com/US/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/stem-cell-culture/mesenchymal-stem-cell-culture-protocols
Hello! Great video! I have a question, you mentioned that before thawing you had 100 millon of cells total in all the vials but when you make the viability test with trypan blue, the automated cell counter throw up an approximate total count of 9 million of cell /mL, so I think that the total amount of cells in the sample was at most 20 millions.
Where were the other 80 million that in theory should have been in all the vials? Other thing, It is not better to have a Biosafety cabinet to prepare the cells for therapy?
I think the 10 microliter sample was taken from one of the 4 vials that went into the 100 million. Or it was diluted so the cell reader can actually give a viable reading. If there's too many cells in the plate it won't read properly. Yes the biosafety cabinet is great but for the purposes of the camera angle, the video, etc we filmed in the area we did.
Where would you recommend going for stem cell treatment? I don’t want to use my cells. I want the cord cells. Thx.
The best place I can recommend is Innate Healthcare Institute obviously. We're the only clinic in the US, that I know of currently, using actual live cord cells.
Thanks for the informative video. How do you know the number of stem cells you are using? Thanks!
The stem cell biologist uses a couple different methods, a hemocytometer and automated cell counters.
So, you have a lab to culture these cells and how long does it take to culture the stem cells?
About 3-4 weeks
What size filter do you use for IV?
70 micron liter
@@InnateHealthcareInstitute thanks!
Have you injected these stem cells in to a bulging disc or a herniated disc and if so what was the success rate?
We don't inject into the disc but around it. Very good success rate in most treatments. Facets and other supporting structures need attention as well.
What is the cost for treatment bulging disk hnp tq?
Once you thaw the cells, can your refreeze them at any point?
Yes you can refreeze them but the more times you thaw and freeze you'll lose viability.
You mentioned using 100 - 300 million cells. I am assuming this is for IV use. For a single injection into a knee joint, for example, how many cells on average would you say would be effective in helping to relieve symptoms of osteoarthritis? Would you consider using a combination of exosomes and MSCs? Thanks
That dose has been used for IV, intranasal, intramuscular, and even intranasal. A knee, depending on severity, age of patient, weight, activity level, overall health, and even how proactive a patient it, could easily have a dose of 50-300 million cells. In many protocols, UC-MSCs and exosomes are used in tandem.
Isn’t it illegal to culture/replicate cells in the USA? Thanks
You should first know that no federal law does or ever did ban stem cell research or use in the United States. Many people ask if the treatments we provide are approved by the FDA or some other agency. First, it is important to understand that the FDA has absolutely no jurisdiction over physicians and clinics. This includes how to practice, treat, or use drugs, devices, or biologics. This activity is governed by state law and is largely left to the independent medical judgment of the practitioner. The FDA actually admits openly that it does not “regulate a physician's or nurse's practice [and] does not tell providers what to do when running their business or what they can or cannot tell their patients.”
The FDA does have some authority over the stem cell industry and regulates it in three ways. First, it regulates stem cell production by laboratories or other manufacturers. These must comply with a rigorous set of safety protocols and manufacturing practices. Second, it regulates claims that manufacturers and physicians make about the use of stem cells, including treatments, cures, efficacy, etc. For example, the federal government does not want medical practices advertising to the public that stem cells are a guaranteed cure for any particular condition.
The third way the FDA regulates the use of stem cells is by classifying them as drugs. If stem cells are taken from a human subject and manipulated or altered in a particular way, the FDA considers this new stem cell creation to be a drug. When this happens, the FDA has authority to regulate these substances, as well as the medical practices using them in procedures.
However, Innate Healthcare Institute does not alter stem cells in this way. It is not creating new “drugs,” but is simply taking stem cells from a donated umbilical cord, which would otherwise be considered a waste product and disposed, and preparing them in a way that is absolutely compliant with pertinent laws and regulations and using them according to its experience, expertise, and independent medical judgment. Federal case law has positively established that the FDA has no jurisdiction when stem cells are not manipulated or altered.
The Institute also does not make any public claims or advertise that its stem cell treatments will cure a particular condition. It looks at treatments on a case-by-case basis and determines if a stem cell protocol may be effective for a particular patient.
State Law
Arizona permits naturopathic physicians to diagnose and treat disease with a wide range of procedures and natural medicines. This includes administering stem cells for therapeutic use. Innate Healthcare Institute uses stem cells manufactured in its own lab as well as in a nationally-recognized academic facility and not manipulated in any meaningful way before use on a patient. Beyond this, almost no state regulation of stem cells exists. The only real way the state regulates stem cell use is through the Board of Medicine to ensure they are used according to the proper standard of care, as with any other procedure.
Effectiveness
Innate Healthcare Institute has clinical proof of beneficial patient outcomes from its stem cell protocols. This includes those with different types of conditions and needs. The Institute can supply written testimonials from these patients. It has been engaged for some time in extensive primary and secondary research to know how best to use stem cells and achieve the greatest possible outcomes. It is committed to continuing its practice based on the highest levels of knowledge, professionalism, and standard of care.
The Institute is also fully committed to staying compliant with all laws, regulations, and guidelines that govern the use of stem cells. It has retained a national law firm that specializes in healthcare and FDA law to guide it through the legal processes and provide the most up-to-date counsel. In this way, you can be sure that Innate Healthcare Institute will always serve its patients within all legal and medical parameters.
I had my hernia treated with a stem cell after sofering for 15 years a reyecten
Operations of my spine
After the inyecciones gradually the pain went alway
These is real it works
The stem cells is the greatest treatment
@@oscarcetraro3057where do u get ur stemcell treatment, and how much the treatment cost tq?
So a very "fair question" for you is what "Specific Lab" do you get these umbilical stem cells from ??? And how can you be 100% positive that they are "Human Umbilical Mesenchymal Stem Cells"??? And not from a mammal??? Lets see if you reply.
Very fair and good question. We're currently using cells from our own lab, built right into our clinic.
Yes they have been characterized for MSC markers, specifically CD 73, 90, and 105 as well as CD 34 negative.
So I ask that you follow up to me in "Laymans Term English". Iam not being a smart alec but need to know do these stem cells come from "HUMAN UMBILICAL MESENCHYMAL CELLS or NOT????????? 99.999% of the people reading your reply DO NOT have any idea what CD 73,90,& 105 as well as CD 34 NEGATIVE even means. PLEASE REPLY AS IAM GIVING SERIOUS CONSIDERATION TO COMING OUT FOR TREATMENTS AND EVEN HAVE A CONSULTATION SCHEDULED. @@InnateHealthcareInstitute In reading your reply it sounds like you are using cells THAT HAVE BEEN "EXPANDED".........................
Do you screen umbilical cord donors for certain qualifications? Can you procure umbilical cords from unvaccinated donors.
How do your stem cells compare or contrast to clinics in Mexico, like Dream Body Clinic, who claim that it’s better to use fresh umbilical cords and cultivate the mesenchymal stem cells for 3 rounds to produce more cells?
Andro Med coming soon to Belarus where we will only be using cells from non vaccinated mothers
Currently all the cords we've used have been frozen BEFORE the covid vaccine was developed, 2015-2019. In the future I think the only way would be to screen if donors were vaccinated or no. If that's a major concern.
Promoting "fresh is better than frozen" is a bit of a marketing ploy. Research isn't conclusive on the topic and some research has shown frozen to 'better' cells. Three passes is pretty standard but I have heard of some clinics going as high as 6. I don't recommend listening to places that claim their "cells are better". How are they measuring this? How can the prove they're better? It's like a coffee shop having a "best cup of coffee in the world" sign up. Until there's more conclusive evidence frozen cells are easier, safer, more affordable and work just as fine or better.
This is a list of my health diagnoses. Can embryonic stem cell fluid help?
Crohn's Disease
Autoimmune disease
Enteropathic arthropathies, multiple sites
Fibromyalgia
Irritable Bowel Syndrome
Macular Degeneration
Spinal Neuropathy
Obstructive Sleep Apnea
Diagnosis of spinal pain
Diagnosis of Pain of sternum bone spurs
osteoarthritis
Palmoplantar pustulosis
Psoriatic Arthritis
Sjögren syndrome
Focused seizures
Please reach out to the clinic to speak to a physician.
Why don't you just dump the entire contents of the small vial into the larger vial with saline? Why use the pipette at all?
You could, once you break up the cell pellet with the pippette. Pippetting does help suspend the cells in the saline so they don't get clumped together. It depends what part of the process you're in. If the cells are well suspended I'll just dump the contents in another vial and then rinse to make sure I'm getting as many cells as possible.