Protein microarray interaction assay

A demonstration of how to detect protein-protein interactions using Arabidopsis protein chips. We also have info on how to test kinase activity directly on the chip in the full protocol which can be found in the Methods and Protocols series in the Plant MAP Kinases book (link below).
link.springer.c...

[@zohasadqat]

This was a great video. Helps to clarify the theory a lot.

[@lizbrauer3951]

Thanks, glad it was helpful!

[@sourabhgurav6665]

Are you analyzing interaction of single type of protein or a mixture of different tagged protein ?

[@lizbrauer3951]

single type of protein though you could use this for a mixture as long as they have different tags

[@einsteinpt1799]

Could you help me ,please
I can’t hear you clearly , what did you use for washing quickly in 5:06

[@lizbrauer3951]

Sure it was deionized water. Check out the protocol in the link below the description for more details. Let me know if you can't get access and I'll send a copy.

[@lizbrauer3951]

Sorry for the super late response! It was deionized water. Hope you were able to figure it out, the written protocol can also be found here: link.springer.com/content/pdf/10.1007/978-1-4939-0922-3_17.pdf 'Experimental and Analytical Approaches to Characterize Plant Kinases Using Protein Microarrays'.